L-arginine impact on cherry rootstock rooting and biochemical characteristics in tissue culture

Abstract

In the present study, the effects of indole-3-butyric acid (IBA) separately and simultaneously with L-arginine on the morphogenic and biochemical responses in the cherry rootstocks CAB-6P (Prunus cerasus L.) and Gisela 6 (Prunus cerasus × Prunus canescens) were investigated. In the CAB-6P rootstock, the best root number and root length results were obtained with 2 mg L -1 IBA plus 0.5 mg L -1 L-arginine and 1 mg L -1 IBA plus 1 mg L -1 L-arginine, respectively. The rooting percentage was highest (100%) with 2 mg L -1 IBA alone or combined with 1 mg L -1 L-arginine. In the Gisela 6 explants, 2 mg L -1 IBA without L-arginine significantly enhanced root number (9.27) and root fresh and dry weight, as well as rooting percentage (100%). Root length was longest (38 mm) in the combination of the lowest IBA (0.5 mg L -1 ) and highest L-arginine (2 mg L -1 ) concentrations. Thus, L-arginine promotes the positive effect of IBA on rooting with regard to both root number and root length in both cherry rootstocks. In the CAB-6P explants, L-arginine combined with IBA had an inhibitory effect on leaf chlorophyll content, whereas in the Gisela 6 rootstock it had absolutely no effect. In the CAB-6P rootstock, depleted levels of proline in roots were observed, showing the osmoregulation and osmotic adjustment mechanisms. The carbohydrate concentration in leaves was greatest with 0.5 mg L -1 IBA plus 0.5 mg L -1 L-arginine, and in roots with 2 mg L -1 IBA plus 0.5 mg L -1 L-arginine. In the Gisela 6 explants, IBA (1 and 2 mg L -1 ) applied both alone and along with L-arginine, as well as the combination of the lowest IBA (0.5 mg L -1 ) and the highest L-arginine (2 mg L -1 ) concentrations, considerably increased leaf carbohydrate concentration compared to the control. In roots, carbohydrate concentration was maximum with 2 mg L -1 IBA plus 0.5 mg L -1 L-arginine. The elevated levels of proline in roots with 1 mg L -1 IBA plus 0.5 mg L -1 L-arginine indicate stressful conditions.

An improved micropropagation protocol for lentisk (Pistacia lentiscus L.)

“…More recently, a study was conducted to determine the effects of gibberellic acid (GA 3 ) and N6-benzyladenine (BA) on in vitro micrografting developed to produce P. lentiscus plantlets (Onay et al, 2016b). Furthermore, different studies have shown that cytokinins, GA 3 and some amino acids can improve cell growth and enhance organ development (Baskaran and Jayabalan, 2007;Kumar and Kumari, 2010;Unek et al, 2010;Malá et al, 2013;Gonbad et al, 2014;Sarropoulou et al, 2014).…”

Effect of IBA and TIBA on rhizogenesis of Wild cherry in vitro

“…Positive effect of IBA on rooting in vitro was found in numerous studies in different species of Prunus sp. (Buyukdemirci, 2008;Šiško, 2011;Canli and Demir, 2014;Sarropoulou et al 2014;Xu et al 2015;Kumar et al 2020;Zamanipour et al 2015). While 2,3,5-triiodobenzoic acid (TIBA) is well known as the inhibitor of polar transport of indol-acetic acid, it could, however, provoke rooting regarding site of application and concentration (McNamara and Mitchell, 1991; Kovačević et al 2013a).…”

Some Urea Derivatives Positively Affect Adventitious Root Formation: Old Concepts and the State of the Art

“…On the other hand, a lot of applied research has been done to enhance adventitious rooting in difficult-to-root species, especially woody ones, by the utilization of bioactive compounds that could integrate or adjuvate the auxin activity. Cuttings have been supplemented with phenolic compounds [21], thiol compounds [22,23], amino acids [24,25], and carbohydrates [26,27] in combination with auxin to stimulate the root formation and to reduce its unfavourable side effects. In fact, it has been reported that adventitious roots may be stunted or malformed, as a result of the auxin treatment alone [28], giving rise to a poor-quality root system that will hardly support the ex vitro transfer and acclimatization.…”