Koyun Oositlerinin İn vitro Maturasyonu (IVM) ve Embriyonal Gelişimi (IVC) Üzerine L-Ergotiyonin ve L-Askorbik Asit’in Etkileri

Öztürkler, Yavuz; Yıldız, Savaş; Güngör, Örsan; Pancarcı, Şükrü Metin; Kaçar, Cihan; Ari, Umut Çağın

doi:10.9775/kvfd.2009.1646

Cited by 1 publication

(5 citation statements)

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“…Similar findings about the effective activities of cis-retinal acids on proportion of oocytes that reached the metaphase II stage and also in vitro embryo production were reported from Spain [20] , India [21] , Iran [22] and United States [23] . Öztürkler et al [2] reported that the percentages of oocyte maturation, cleavage, 8-cell and morula stage embryos in L-ergothioneine supplemented group were significantly higher compared with the other groups. They showed that the percentages of oocyte maturation in L-ergothioneine group and L-ascorbic acid group were 2.67 and 1.22 times higher than control group, respectively.…”

Section: Discussionmentioning

confidence: 98%

“…The effects of retinol as an antioxidant in IVP on nuclear maturation rates, cleavage rates, and blastocyst development were examined by some researchers [1][2][3][4]8,13,18] . This study evaluated different concentration of 9-cis-retinal acid on the efficacy of bovine IVP media is examined in a study and results revealed that the addition of 9-cis-retinal acid into the IVM medium increased bovine blastocyst development and hatching rate [12] which were similar to our findings.…”

Section: Discussionmentioning

confidence: 99%

“…The procedures of the IVP of embryo are consist of an in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC) of embryos. IVP is a sequential process and failure in each step may influence the final result [2][3][4] . One of the most important factors with detrimental effects on the quality of gametes and embryos under culture conditions is oxidative stress (OS) [2][3][4] .…”

Section: Introductionmentioning

confidence: 99%

“…IVP is a sequential process and failure in each step may influence the final result [2][3][4] . One of the most important factors with detrimental effects on the quality of gametes and embryos under culture conditions is oxidative stress (OS) [2][3][4] . Oxidative stress induces mitochondrial, DNA, RNA, and protein damages [2][3][4] , inhibition of sperm-oocyte fusion [2][3][4] and finally embryo cell apoptosis [2][3][4] .…”

Section: Introductionmentioning

confidence: 99%

“…One of the most important factors with detrimental effects on the quality of gametes and embryos under culture conditions is oxidative stress (OS) [2][3][4] . Oxidative stress induces mitochondrial, DNA, RNA, and protein damages [2][3][4] , inhibition of sperm-oocyte fusion [2][3][4] and finally embryo cell apoptosis [2][3][4] . Free radicals such as reactive oxygen species (ROS) interact with oocytes and embryos and decrease blastulation rates in embryo development [2][3][4] .…”

Section: Introductionmentioning

confidence: 99%

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Sığır İn Vitro Embriyo Üretiminde Sistamin ve 13-Cis-Retinoik Asitin Etkisi

Ranjbar¹,

Eslampour²,

Moghadam³

2018

Kafkas Univ Vet Fak Derg

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One of the main factors decreasing the success of an in vitro embryo production is oxidative stress-mediated reactive oxygen species resulting in cellular damages. The present study was carried out to evaluate the effects of addition of different doses of cysteamine and 13-cis-retinoic acid during in vitro maturation on oocyte maturation and blastocyst formation. Five-hundred oocyte complexes were randomly collected from slaughterhouse and assigned to five groups; oocytes matured in vitro maturation (IVM) medium without any supplementation (control), IVM medium supplemented with 50 µM or 100 µM cysteamine, IVM supplemented with 5 nM 13-cis-retinoic acid and IVM supplemented with 10 nM 13-cis-retinoic acid. Matured oocytes were fertilized and cultured. Oocytes and embryos were evaluated for nuclear maturationand blastocyst formation, respectively. The highest numbers of oocytes in the cumulus expansion, cleavage, murola and blastocyst stages were seen in cows treated with 13-cis retinoic (5 nm) (92%), cysteamine (100 µL) (74%), cysteamine (50 and 100 µL) (48%), cysteamine (100 µL) and 13-cis retinoic (24%) and finally cysteamine (100 µL) (22%), respectively.Nuclear maturation of oocytes decreased significantly in the media supplemented with 10 nM 13-cis-retinoic acid (P<0.05). The results of the present study demonstrated that IVM supplementation with cysteamine and a low concentration of 13-cis-retinoic acid improved the efficacy of an in vitro production of bovine embryo.

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