Introduction: The most common sexually transmitted infection worldwide is the human papillomavirus (HPV) infection. It is believed that over 80% of sexually active women have contracted the virus at some point in their lives. About 11 million women in Saudi Arabia who are aged 15 to 44 years are at risk of having HPV-related cervical cancer. Aim: To assess the awareness and knowledge of undergraduate medical students (males and females) about the human papilloma virus (HPV) vaccination and to pinpoint the major factors influencing their attitudes towards it. Subjects and methods: A prospective, cross-sectional online survey included 411 undergraduate medical students (males and females) across different grades during three months, from January 1st to March 31st, 2024, at the Batterjee Medical College, Jeddah City, Saudi Arabia. We statistically analyzed the collected data using Microsoft Office®, Excel® 2016, and IBM's Statistical Package for Social Studies (SPSS®) version 26.0. IBM is located in Chicago, Illinois, USA. Results: The majority of participating students were of young age, 20–25 years old (233, 56.69%), female (393, 95.62%), and of Saudi nationality (260, 63.26%). The most common recruited students were from the preparatory year (124, 30.17%), while the fewest were from the M6 grade (6, 1.46%). We implemented "Kamel's scoring system for students' awareness", which yielded the following scores: not acceptable (124, 30.17%), fairly acceptable (101, 24.57%), moderately acceptable (95, 23.11%), acceptable (71, 17.27%), and highly acceptable (20, 4.87%). Next, we implemented "Kamel's scoring system for students' knowledge", which yielded the following scores: very low (130, 31.63%), low (110, 26.76%), moderate (90, 21.90%), high (50, 12.17%), and very high (31, 7.54%). We implemented "Kamel's scoring system for students' attitude" to assess the students' attitude towards HPV vaccine utilization. We interpreted the calculated scores as inadequate or poor (136, 33.09%), reasonable or fair (100, 24.33%), sufficient or good (80, 19.46%), remarkable or very good (70, 17.03%), and outstanding or excellent (25, 6.08%). results were of statistical significance (the P-value is <0.00001). Conclusion: The estimated degree of awareness and knowledge about the HPV vaccination among undergraduate medical students at the Batterjee Medical College (BMC) was poor. This results in low motivation and utilization of the available vaccine.
