Knockdown of LncRNA SCAMP1 suppressed malignant biological behaviours of glioma cells via modulating miR‐499a‐5p/LMX1A/NLRC5 pathway

Abstract

Dysregulation of long non‐coding RNAs (lncRNAs) confirm that it plays a crucial role in tumourigenesis and malignant progression of glioma. The present study demonstrated that LncRNA secretory carrier membrane protein 1 (SCAMP1) was up‐regulated and functioned as an oncogene in glioma cells. In addition, miR‐499a‐5p was down‐regulated meanwhile exerted tumour‐suppressive function in glioma cells. Subsequently, inhibition of SCAMP1 significantly restrained the cell proliferation, migration and invasion, as well as promoted apoptosis by acting as a molecular sponge of miR‐499a‐5p. Transcription factor LIM homeobox transcription factor 1, alpha (LMX1A) was overexpressed in glioma tissues and cells. Moreover, miR‐499a‐5p targeted LMX1A 3′‐UTR in a sequence‐specific manner. Hence, down‐regulation of SCAMP1 remarkably reduced the expression level of LMX1A, indicating that LMX1A participated in miR‐499a‐5p‐induced tumour‐suppressive effects on glioma cells. Furthermore, knockdown of LMX1A decreased NLR family, CARD domain containing 5 (NLRC5) mRNA and protein expression levels through directly binding to the NLRC5 promoter region. Down‐regulation of NLRC5 obviously inhibited malignant biological behaviours of glioma cells through attenuating the activity of Wnt/β‐catenin signalling pathway. In conclusion, our study clarifies that SCAMP1/miR‐499a‐5p/LMX1A/NLRC5 axis plays a critical role in modulating malignant progression of glioma cells, which provide a novel therapeutic strategy for glioma treatment.

Bioinformatics analysis of the regulatory lncRNA-miRNA-mRNA network in patients with neuropathic pain and prediction of potential therapeutics

“…Several vital lncRNAs were found in the present NP lncRNA-miRNA-mRNA network, and these lncRNAs have not been reported in NP. For example, lncRNA SCAMP1 played an important role in the regulatory network of this study, and it had been reported in a previous study that it may be associated with malignant biological behavior (40). In addition, lncRNA SCAMP1 discovered in the present study were related to the regulation of cancer (41).…”

Long non‑coding RNA EWSAT1 contributes to the proliferation and invasion of glioma by sponging miR‑152‑3p

“…Previous studies have reported that lncRNAs are potential miRNA sponges (33,34). Silencing of the lncRNA SCAMP1 inhibits malignant biological behavior in glioma through the miR-499a-5p/LIM homeobox transcription factor 1 alpha/NLR family CARD domain containing 5 signaling pathway (35). HOX transcript antisense RNA directly inhibited WIF-1 level by regulating the promoter region methylation of its histone H3K27, and then activating the Wnt/β-catenin signaling pathway (36).…”

Bioinformatics Analysis of the Regulatory lncRNA-miRNA-mRNA Network in Patients with Neuropathic Pain

“…Similarly, the lncRNA found in this article has not been fully reported in previous studies. It is reported that knockdown of lncRNA SCAMP1 suppressed malignant biological behaviors of glioma cells through regulating the miR-499a-5p/LMX1A/NLRC5 pathway (20). Additionally, SCAMP1 could be bound with miR-137 and accelerate the progression of ovarian cancer (OC) (21).…”