Knockdown of cZNF292 suppressed hypoxic human hepatoma SMMC7721 cell proliferation, vasculogenic mimicry, and radioresistance

Yang, Wei; Liu, Yingying; Gao, Ruoling; Xiu, Zenghe; Sun, Ting

doi:10.1016/j.cellsig.2019.04.011

Cited by 48 publications

(46 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further, silencing cZNF292 repressed tube formation through the mobilization of Wnt/b-catenin pathway [27]. Another research elucidated that cZNF292 knockdown repressed proliferation, radioresistance and vasculogenic mimicry (VM) of hypoxic human hepatoma cells [28]. In addition, it was unclosed that hypoxia caused the reduction of cZNF292.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA cZNF292 silence alleviates OGD/R-induced injury through up-regulation of miR-22 in rat neural stem cells (NSCs)

Cao¹,

Liu²,

Zhang³

et al. 2020

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

Background: Hypoxic-ischaemic encephalopathy (HIE) is a prevailing severe brain damage disease in newborns, and caused by perinatal asphyxia cerebral ischaemia and reperfusion. Here, we investigated the role of cZNF292 in oxygen-glucose deprivation/reperfusion (OGD/R)-induced neural stem cells (NSCs) injury, and explored the underlying molecular mechanism. Methods: Before NSCs were subjected to OGD/R treatment, NSCs were transfected with or without overexpressing cZNF292, si-cZNF292 or miR-22 inhibitor. Viability, apoptosis and potential molecular mechanism were examined. Cell viability and apoptotic rate were evaluated utilizing cell counting kit-8 (CCK-8) and flow cytometry. The cZNF292 and miR-22 expression was determined utilizing quantitative reverse transcription-PCR (qRT-PCR). Moreover, apoptosis and Wnt/b-catenin and PKC/ERK pathwaysassociated proteins were quantified applying western blot. Results: OGD/R repressed viability and promoted apoptosis of NSCs. Also, cZNF292 expression was promoted by OGD/R treatment. Moreover, cZNF292 overexpression further caused OGD/R-stimulated damage. Inversely, silencing cZNF292 alleviated OGD/R-stimulated damage in NSCs. In addition, miR-22 expression was negatively regulated by cZNF292. It was confirmed that silencing cZNF292 attenuated OGD/R-induced NSCs injury and promoted the activation of Wnt/b-catenin and PKC/ERK pathways via the up-regulation of miR-22. Conclusions: The cZNF292 silence alleviated OGD/R-induced injury through the up-regulation of miR-22 in NSCs, and which furnished the theoretical basis for further research on HIE progression.

show abstract

Section: Discussionmentioning

confidence: 99%

Circular RNA cZNF292 silence alleviates OGD/R-induced injury through up-regulation of miR-22 in rat neural stem cells (NSCs)

Cao¹,

Liu²,

Zhang³

et al. 2020

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

show abstract

“…Hypoxia also induced upregulation of circ-0010729, which was shown to regulate vascular endothelial cell proliferation and apoptosis via targeting the miR-186/HIF1α axis [104]. Interestingly, another study indicated that cZNF292 was induced by hypoxia in a time-dependent manner in hepatoma cells independent of HIF1α, promoting hypoxic hepatoma proliferation, VM, and radioresistance [103]. As hypoxic microenvironment-related circRNAs participate in angiogenesis, metastasis, invasion, and resistance to radiation therapy (Fig.…”

Section: Hypoxia Regulates Circrna Productionmentioning

confidence: 97%

“…Interestingly, while further exploring the mechanism of the angiogenic function of cZNF292, researchers found that cZNF292 had neither a cisregulatory function in host gene expression nor a putative function as a miRNA sponge [102]. Further investigation showed that cZNF292 knockdown inhibited hepatoma vasculogenic mimicry (VM) and radioresistance in vitro and in vivo by increasing sex-determining region Y (SRY)-box 9 (SOX9) nuclear translocation, subsequently reducing Wnt/β-catenin signalling pathway activity [103].…”

Section: Promoting Angiogenesismentioning

confidence: 99%

“…In addition, the hypoxic microenvironment makes cancer cells more resistant to radiotherapy [148]. Hypoxia-induced cZNF292 can enhance the radioresistance of hypoxic hepatoma cells [103]. Notably, the presence of CSCs and angiogenesis are closely associated with therapeutic resistance [149][150][151].…”

Section: The Clinical Potential Of Circrnas Circrnas Regulate Cancer mentioning

confidence: 99%

See 1 more Smart Citation

Roles of circRNAs in the tumour microenvironment

et al. 2020

View full text Add to dashboard Cite

The tumour microenvironment (TME) constitutes the area surrounding the tumour during its development and has been demonstrated to play roles in cancer-related diseases through crosstalk with tumour cells. Circular RNAs (circRNAs) are a subpopulation of endogenous noncoding RNAs (ncRNAs) that are ubiquitously expressed in eukaryotes and have multiple biological functions in the regulation of cancer onset and progression. An increasing number of studies have shown that circRNAs participate in the multifaceted biological regulation of the TME. However, details on the mechanisms involved have remained elusive until now. In this review, we analyse the effects of circRNAs on the TME from various perspectives, including immune surveillance, angiogenesis, hypoxia, matrix remodelling, exo-circRNAs and chemoradiation resistance. Currently, the enormous potential for circRNA use in targeted therapy and as noninvasive biomarkers have drawn our attention. We emphasize the prospect of targeting circRNAs as an essential strategy to regulate TME, overcome cancer resistance and improve therapeutic outcomes.

show abstract

“…Silencing cZNF292 inhibits radiation-induced γ-H2AX foci formation due to its binding with SOX9 protein to inhibit the activity of the β-catenin-mediated DNA repair pathway. This ultimately enhances the sensitivity of HCC cells to radiotherapy [106].…”

Section: Hepatocellular Carcinomamentioning

confidence: 99%

Functions and mechanisms of circular RNAs in cancer radiotherapy and chemotherapy resistance

et al. 2020

View full text Add to dashboard Cite

Circular RNAs (circRNAs), one type of non-coding RNA, were initially misinterpreted as nonfunctional products of pre-mRNA mis-splicing. Currently, circRNAs have been proven to manipulate the functions of diverse molecules, including non-coding RNAs, mRNAs, DNAs and proteins, to regulate cell activities in physiology and pathology. Accumulating evidence indicates that circRNAs play critical roles in tumor genesis, development, and sensitivity to radiation and chemotherapy. Radiotherapy and chemotherapy are two primary types of intervention for most cancers, but their therapeutic efficacies are usually retarded by intrinsic and acquired resistance. Thus, it is urgent to develop new strategies to improve therapeutic responses. To achieve this, clarification of the underlying mechanisms affecting therapeutic responses in cancer is needed. This review summarizes recent progress and mechanisms of circRNAs in cancer resistance to radiation and chemotherapy, and it discusses the limitations of available knowledge and potential future directions.

show abstract

Knockdown of cZNF292 suppressed hypoxic human hepatoma SMMC7721 cell proliferation, vasculogenic mimicry, and radioresistance

Cited by 48 publications

References 49 publications

Circular RNA cZNF292 silence alleviates OGD/R-induced injury through up-regulation of miR-22 in rat neural stem cells (NSCs)

Circular RNA cZNF292 silence alleviates OGD/R-induced injury through up-regulation of miR-22 in rat neural stem cells (NSCs)

Roles of circRNAs in the tumour microenvironment

Functions and mechanisms of circular RNAs in cancer radiotherapy and chemotherapy resistance

Contact Info

Product

Resources

About