KLF8 is associated with poor prognosis and regulates glycolysis by targeting GLUT4 in gastric cancer

Mao, Anwei; Zhou, Xiang; Liu, Yanxia; Ding, Jian; Miao, Aiyu; Pan, Gaofeng

doi:10.1111/jcmm.14378

Cited by 45 publications

(27 citation statements)

References 41 publications

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“…SLC2A4 is an efficient glucose transporter that is located in cytoplasmic vesicles and can be transferred to the plasma membrane to take up glucose when stimulated by insulin [ 21 ]. It has been shown that SLC2A4 expression is elevated during cancer progression [ 22 ], 23 ], consistent with our results. SLC2A4 inhibition abolished the biological effects of miR-302b-3p downregulation on inducing the proliferation, migration and invasion of PC cells.…”

Section: Discussionsupporting

confidence: 93%

LncRNA SNHG16 contributes to tumor progression via the miR-302b-3p/SLC2A4 axis in pancreatic adenocarcinoma

Miao

et al. 2021

Cancer Cell Int

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Background It has been reported that the lncRNA SNHG16 has significantly increased expression in pancreatic adenocarcinoma (PC). However, the functions and mechanisms of SNHG16 are not clear. The aim of this study was to explore the effects of SNHG16 on PC. Methods qRT-PCR analysis was applied to detect the expression levels of SNHG16, miR-302b-3p and SLC2A4 in PC tissues and cells. CCK8 and EdU assays were used to evaluate the proliferation of PC cells. Transwell assays were used to assess PC cell migration and invasion. Apoptosis was evaluated by flow cytometry, and the expression of apoptosis-related proteins (including Bax, Bcl-2, cleaved caspase-3 and cleaved caspase-9) was tested by western blotting. The interactions between miR-302b-3p and SNHG16 or miR-302b-3p and the 3’UTR of SLC2A4 mRNA were clarified by a dual luciferase reporter assay and RNA immunoprecipitation. Results SNHG16 expression was significantly elevated in PC tissues and cell lines and was associated with poor prognosis of PC patients. Knockdown of SNHG16 reduced PC cell proliferation, migration and invasion. SNHG16 acted as a sponge to regulate miR-302b-3p expression in PC cells. In addition, miR-302b-3p targeted SLC2A4 directly. Conclusions SNHG16 promoted the progression of PC via the miR-302b-3p/SLC2A4 axis and was expected to be a potential target for the early diagnosis and treatment of PC.

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Section: Discussionsupporting

confidence: 93%

LncRNA SNHG16 contributes to tumor progression via the miR-302b-3p/SLC2A4 axis in pancreatic adenocarcinoma

Miao

et al. 2021

Cancer Cell Int

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“…An accurate prediction of the prognosis of patients with GC plays a very important role in postoperative treatment and follow-up planning. For resectable GC, the prognosis is related not only to the TNM stage (In et al 2017) and biological characteristics (Mao et al 2019;Sun et al 2020;Wang et al 2019) but also to the degree of LND (Degiuli et al 2004;Enzinger et al 2007;Schwarz and Smith 2007) and postoperative comprehensive treatment (Smyth et al 2020). However, so far, except for the extent of lymphadenectomy at the time of gastrectomy, only ELNs and NLNs can reflect the degree of LND to some extent.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, T stage is a robust risk factor in GC, which is based on the depth of tumor invasion and can represent the major tumor characteristics. Increasing studies have shown that T stage is not only related to prognosis but also closely related to tumor biological characteristics (Mao et al 2019;Sun et al 2020;Wang et al 2019). Both NLNs and T stage are important independent prognostic factors in GC, which represent the degree of LND and the severity of disease, respectively.…”

Section: Introductionmentioning

confidence: 99%

The log odds of negative lymph nodes/T stage: a new prognostic and predictive tool for resected gastric cancer patients

Xie

Pang

et al. 2021

J Cancer Res Clin Oncol

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Purpose When only the TNM classification is used to predict survival in gastric cancer (GC) patients, the impact of the degree of lymphadenectomy on the prognosis is neglected. This study aimed to establish a more effective nomogram based on the log odds of negative lymph nodes/T stage ratio (LONT) to predict survival in surgically treated GC patients. Methods The data of resected GC patients were extracted from the Surveillance, Epidemiology, and End Results Program (SEER) database. Univariate and multivariate Cox regression analyses were used to identify the significant prognostic factors. The prognostic performance was assessed using a calibration plot, concordance index (C-index), and area under the (time-dependent receiver operating characteristic) curve (AUC) to compare the predicted survival probability based on the nomogram score groups. Results The results showed LONT as an independent prognostic factor for cancer-specific survival (CSS) and overall survival (OS), independent of clinicopathological factors. After removing potential redundancy, only LONT, T stage, N stage, location and age were used in the final nomogram model. The model had a higher C-index (0.736 ± 0.012) and AUC (0.798) than the TNM staging system (0.685 ± 0.012 and 0.744). The nomogram score could predict a significant survival difference between any two adjacent groups in terms of CSS and OS. Conclusion High LONT is associated with improved survival of gastric cancer patients, independent of other clinicopathological factors. The prognostic nomogram model based on LONT could effectively predict CSS and OS for resectable GC patients.

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“…Lactate production and glucose consumption were measured by the Lactate Colorimetric Assay Kit (Merck Millipore, MA, USA)) and Glucose Uptake Colorimetric Assay Kit (Sigma-Aldrich, MO, USA), respectively, according to the manufacturer's protocols and previously described (Mao et al 2019 ). RT-PCR was applied to detect the glycolytic enzymes expression levels.…”

Section: Methodsmentioning

confidence: 99%

LINC00242/miR-1-3p/G6PD axis regulates Warburg effect and affects gastric cancer proliferation and apoptosis

Deng

et al. 2021

Mol Med

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Background Reprogrammed glucose metabolism of enhanced Warburg effect (or aerobic glycolysis) is considered as a hallmark of cancer. Long non-coding RNAs (lncRNAs) have been certified to play a crucial role in tumor progression. The current study aims to inquire into the potential regulatory mechanism of long intergenic non-protein coding RNA 242 (LINC00242) on aerobic glycolysis in gastric cancer. Method LINC00242, miR-1-3p and G6PD expression levels in gastric cancer tissues and cells were determined by qRT-PCR. Cell apoptosis or viability were examined by Flow cytometry or MTT assay. Western blot was utilized to investigate G6PD protein expression levels. Immunohistochemical (IHC) and hematoxylin and eosin (H&E) staining were used for histopathological detection. The targeted relationship between LINC00242 or G6PD and miR-1-3p was verified by luciferase reporter gene assay. Nude mouse xenograft was utilized to detect tumor formation in vivo. Result LINC00242 and G6PD was high-expressed in gastric cancer tissues and cells, and LINC00242 is positively correlated with G6PD. Silencing of LINC00242 or G6PD within gastric cancer cells prominently inhibited cell proliferation and aerobic glycolysis in vitro and relieved the tumorigenesis of gastric cancer in vivo. miR-1-3p was predicted to directly target both LINC00242 and G6PD. Overexpression of miR-1-3p suppressed gastric cancer cells proliferation and aerobic glycolysis. LINC00242 competitively combined miR-1-3p, therefore relieving miR-1-3p-mediated suppression on G6PD. Conclusion LINC00242 plays a stimulative role in gastric cancer aerobic glycolysis via regulation of miR-1-3p/ G6PD axis, therefore affecting gastric cancer cell proliferation.

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KLF8 is associated with poor prognosis and regulates glycolysis by targeting GLUT4 in gastric cancer

Cited by 45 publications

References 41 publications

LncRNA SNHG16 contributes to tumor progression via the miR-302b-3p/SLC2A4 axis in pancreatic adenocarcinoma

LncRNA SNHG16 contributes to tumor progression via the miR-302b-3p/SLC2A4 axis in pancreatic adenocarcinoma

The log odds of negative lymph nodes/T stage: a new prognostic and predictive tool for resected gastric cancer patients

LINC00242/miR-1-3p/G6PD axis regulates Warburg effect and affects gastric cancer proliferation and apoptosis

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