Kinetics of Indigenous Nitrate Reducing Sulfide Oxidizing Activity in Microaerophilic Wastewater Biofilms

Villahermosa, D.; Corzo, Alfonso; García‐Robledo, Emilio; González, Juan M.; Papaspyrou, Sokratis

doi:10.1371/journal.pone.0149096

Cited by 9 publications

(8 citation statements)

References 68 publications

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“…The kinetic framework used connects whole‐cell catalysis in flow systems to traditional Michaelis‐Menten kinetics (Eq. 1) between a substrate molecule S and a catalytic bacteria in a biofilm, denoted by E, due to historical relation to enzyme kinetics ,

true normalE + normalS \leftrightarrow_{{k^{'}}_{normala}}^{{normalk}_{normala}} ES \to_{}^{{normalk}_{cat}} normalP + normalE

…”

Section: Figurementioning

confidence: 99%

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A Generalized Kinetic Framework Applied to Whole‐Cell Bioelectrocatalysis in Bioflow Reactors Clarifies Performance Enhancements for Geobacter Sulfurreducens Biofilms

et al. 2019

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A common kinetic framework for studies of whole‐cell catalysis is vital for understanding and optimizing bioflow reactors. In this work, we demonstrate the applicability of a flow‐adapted version of Michaelis‐Menten kinetics to an electrocatalytic bacterial biofilm. A three‐electrode microfluidic biofilm flow reactor measured increased turnover rates by as much as 50 % from a Geobacter sulfurreducens biofilm as flow rate was varied. Based on parameters from the applied kinetic framework, flow‐induced increases to turnover rate, catalytic efficiency and device reaction capacity could be linked to an increase in catalytic biomass. This study demonstrates that a standardized kinetic framework is critical for quantitative measurements of new living catalytic systems in flow reactors and for benchmarking against well‐studied catalytic systems such as enzymes.

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true normalE + normalS \leftrightarrow_{{k^{'}}_{normala}}^{{normalk}_{normala}} ES \to_{}^{{normalk}_{cat}} normalP + normalE

…”

Section: Figurementioning

confidence: 99%

“…1) between a substrate molecule S and a catalytic bacteria in a biofilm, denoted by E, due to historical relation to enzyme kinetics. [16,17]…”

Section: A Generalized Kinetic Framework Applied To Whole-cell Bioelementioning

confidence: 99%

A Generalized Kinetic Framework Applied to Whole‐Cell Bioelectrocatalysis in Bioflow Reactors Clarifies Performance Enhancements for Geobacter Sulfurreducens Biofilms

et al. 2019

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“…Specifically, transects of the cores that had supposed sulfate reduction activity (based on sulfide detection) and denitrification activity (based on acetylene block activity assays) were spatially separated (K. De León, E. Majumder, and F. von Netzer, personal communication, December 2020). However, there are notable exceptions, such as certain bacteria that couple nitrate reduction to sulfide oxidation and thrive under conditions where both nitrate and sulfide are present (49). Additionally, the observation that I. calvum was capable of recovering from reduced sulfur stress suggests that NRB have developed mechanisms to overcome the inhibitory effects of moderate cysteine and sulfide levels and coexist with SRB in some environments.…”

Section: Discussionmentioning

confidence: 99%

Sulfur Metabolites Play Key System-Level Roles in Modulating Denitrification

et al. 2021

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Competition between nitrate-reducing bacteria (NRB) and sulfate-reducing bacteria (SRB) for resources in anoxic environments is generally thought to be governed largely by thermodynamics. It is now recognized that intermediates of nitrogen and sulfur cycling (e.g., hydrogen sulfide, nitrite, etc.) can also directly impact NRB and SRB activities in freshwater, wastewater, and sediment and therefore may play important roles in competitive interactions. Here, through comparative transcriptomic and metabolomic analyses, we have uncovered mechanisms of hydrogen sulfide- and cysteine-mediated inhibition of nitrate respiratory growth for the NRB Intrasporangium calvum C5. Specifically, the systems analysis predicted that cysteine and hydrogen sulfide inhibit growth of I. calvum C5 by disrupting distinct steps across multiple pathways, including branched-chain amino acid (BCAA) biosynthesis, utilization of specific carbon sources, and cofactor metabolism. We have validated these predictions by demonstrating that complementation with BCAAs and specific carbon sources relieves the growth inhibitory effects of cysteine and hydrogen sulfide. We discuss how these mechanistic insights give new context to the interplay and stratification of NRB and SRB in diverse environments. IMPORTANCE Nitrate-reducing bacteria (NRB) and sulfate-reducing bacteria (SRB) colonize diverse anoxic environments, including soil subsurface, groundwater, and wastewater. NRB and SRB compete for resources, and their interplay has major implications on the global cycling of nitrogen and sulfur species, with undesirable outcomes in some contexts. For instance, the removal of reactive nitrogen species by NRB is desirable for wastewater treatment, but in agricultural soils, NRB can drive the conversion of nitrates from fertilizers into nitrous oxide, a potent greenhouse gas. Similarly, the hydrogen sulfide produced by SRB can help sequester and immobilize toxic heavy metals but is undesirable in oil wells where competition between SRB and NRB has been exploited to suppress hydrogen sulfide production. By characterizing how reduced sulfur compounds inhibit growth and activity of NRB, we have gained systems-level and mechanistic insight into the interplay of these two important groups of organisms and drivers of their stratification in diverse environments.

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“…The efficiency of the process (aerobic) depends on influent sulfide concentration and aeration rate, thus need more of the aerobic isolate (B1). While B2 being a spore-forming and facultative anaerobe was needed as a supportive isolate against the inhibitory effect of repulsive components of the PRW and toxic metabolites as reported (Villahermosa et. al., 2016).…”

Section: Characterization and Quality Analysis Of Petroleum Refinery mentioning

confidence: 99%

“…Initially, aliquots samples were withdrawn terminally in 35 ml sampling tubes and transferred to Karnovsky's fixative solution for primary fixation as described previously (Patel et. al., 2016;Villahermosa et. al., 2016) as modified in (Reshma, & Mathew, 2014).…”

Section: Sem-edxsmentioning

confidence: 99%

Integrated co-cultured bacterial strains capabilities to aqueous sulfide and chemical oxygen demand mitigation from high-strength petroleum refinery wastewater

Mani¹,

Aziz²

2019

Nig. J. Biotechnol

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The heterogeneous nature of petroleum refinery based wastewater (PRW) couple with the documented paucity of the classical physicochemical mitigation approaches were behind the quest for a cost-effective and ecofriendly alternative with minimum negative effects. The toxic and inhibitory substances contained in PRW have made its treatment strenuous using a simple pure culture. A novel integrated bacterial mixed culture (BMC), Pseudomonas putida (ATCC 49128) and Bacillus cereus (ATCC 14579) with traceable imprints in biodegradation of high-strength PRW was proposed as a suitable alternative for sulfide oxidation and COD reduction with a potential alternative to classical physicochemical mitigation approaches. Comparative biodegradation potential of two acclaimed bacteria mixed-culture regimen was evaluated. The degree of inhibition cast was assessed based on the effectiveness of BMC to remove the targeted compounds (aqueous hydrogen sulfide and COD) within the assigned period of 8 hours hydraulic retention time (HRT). The PRW was found to contained initial concentration of 8,155 ppm COD, and 500 ppm sulfide, coupled with other refractory substances. The experiment was repeated batch-wise under defined optimal conditions of 0.25 L/min O , 2 o the temperature of 30 C, agitation of 140 rpm at 8 hours retention time. The results obtained indicated 97.47±1.7 % (mg/L) COD reduction and 99.75±1.8 (ppm) sulfide removal efficiency (RE). In addition, the SEM analysis revealed further the potential of BMC to degrade sulfide from PRW to economically feasible elemental sulfur species, while EDXS demonstrated a proportionate elemental composition revealing appreciable sulfur content and high organic carbon accumulation. Despite the expected toxicity and inhibitory effect of the medium, an overwhelming biodegradation was achieved disproportionately, hence the model can be suggested for further research of repulsive wastewaters.

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Kinetics of Indigenous Nitrate Reducing Sulfide Oxidizing Activity in Microaerophilic Wastewater Biofilms

Cited by 9 publications

References 68 publications

A Generalized Kinetic Framework Applied to Whole‐Cell Bioelectrocatalysis in Bioflow Reactors Clarifies Performance Enhancements for Geobacter Sulfurreducens Biofilms

A Generalized Kinetic Framework Applied to Whole‐Cell Bioelectrocatalysis in Bioflow Reactors Clarifies Performance Enhancements for Geobacter Sulfurreducens Biofilms

Sulfur Metabolites Play Key System-Level Roles in Modulating Denitrification

Integrated co-cultured bacterial strains capabilities to aqueous sulfide and chemical oxygen demand mitigation from high-strength petroleum refinery wastewater

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