Kinetic Study on Streptolysin O*

Kanbayashi, Yoshinori; Hotta, Makoto; Koyama, Jiro

doi:10.1093/oxfordjournals.jbchem.a129759

Cited by 18 publications

(11 citation statements)

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“…3) also gave a value of 5 or 4 for the hits required for the hemolysis of streptolysin 0. A value of about 4 was also obtained for streptolysin 0-mediated lysis ofrabbit erythrocytes, which were used for the target cells by Alouf and Raynaud (1) and by Kanbayashi et al (8).…”

Section: Resultsmentioning

confidence: 95%

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Evidence for a one-hit theory in the immune bactericidal reaction and demonstration of a multi-hit response for hemolysis by streptolysin O and Clostridium perfringens theta-toxin

Inoue¹,

Akiyama²,

Kinoshita³

et al. 1976

Infect Immun

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An analytical method was developed for estimating the number of hits necessary to lyse or kill cells in which various concentrations of the cells are treated with a constant amount of the lytic or killing agent in a constant reaction volume. The reaction may be due to a single-component agent or occur by a sequential chain of reactions due to a multi-component agent, even including side, abortive, or counter-reactions. It was clearly shown by this method that immune bactericidal reactions followed a one-hit theory. It was shown by this method that streptolysin 0 required four or five hits for hemolysis and Clostridium perfringens 0-toxin required two hits. These results were confirmed by both logarithmic dose-response and survival analyses. It was also shown that streptolysin 0 and 0-toxin can act complementarily on accumulation of the hits for hemolysis.

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Section: Resultsmentioning

confidence: 95%

“…These two hemolytic toxins are both oxygen labile and related immunologically (11). Streptolysin 0 requires multi-hit for hemolysis and does not turn over when it acts on erythrocytes (1,8). Therefore, these toxins are considered suitable for testing the hit-response analysis.…”

mentioning

confidence: 99%

Evidence for a one-hit theory in the immune bactericidal reaction and demonstration of a multi-hit response for hemolysis by streptolysin O and Clostridium perfringens theta-toxin

Inoue¹,

Akiyama²,

Kinoshita³

et al. 1976

Infect Immun

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“…On the other hand, Cohen et al (5) found that oxidized pneumolysin reacted with cholesterol more slowly than did the reduced form, but he also concluded that the sulfhydryl group remained free after the interaction of cholesterol with reduced toxin. In the studies of Kanbayashi et al (15) it was reported that both the oxidized and reduced forms of streptolysin 0 were adsorbed by cholesterol particles although the "affinity" of the oxidized toxin was less than that of the reduced toxin.…”

Section: Resultsmentioning

confidence: 99%

Binding of cholesterol by sulfhydryl-activated cytolysins

Johnson¹,

Geoffroy²,

Alouf³

1980

Infect Immun

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The binding of cholesterol by pneumolysin, alveolysin, and streptolysin 0 has been demonstrated. The properties of the cytolysin-cholesterol interaction parallel those of cytolysin-erythrocyte interaction in that the reaction is rapid, temperature independent, decreased at elevated pH, and shows the same specificity with respect to other related sterols. However, oxidized or p-hydroxymercuribenzoate-treated toxin showed no decrease in cholesterol-binding activity, whereas the ability of cytolysin to bind to erythrocytes was modified by such treatment. One of the several properties common to that group of cytolytic agents known as the sulflydryl (or thiol)-activated toxins is sensitivity to inhibition by cholesterol and certain related sterols (see review by Bernheimer, 4). These agents act only on cells containing cholesterol (3), and it has been shown with one member ofthe group, cereolysin, that the interaction of toxin with liposomes depends on their cholesterol content (6). The work cited above supports the hypothesis that target cell membrane cholesterol is the receptor for the sulfhydryl-activated toxins. Although several reports of in vitro interactions between purified sulfhydryl-activated toxins and sterol dispersions h4ve appeared (7, 18, 21), there has been no direct demonstration of the binding of pure toxin with cholesterol. In the present communication we present evidence for such a binding and describe some properties of the system. MATERIALS AND METHODS Reagents. Cholesterol (cholest-5-en-3,8-ol) was obtained from Sigma Chemical Co. and from Steraloids, Ltd, [3H]cholesterol with a specific activity of 50 Ci/ mmol from New England Nuclear Corp. and the Commissariat de l'Energie Atomique (France), epicholesterol (cholest-5-en-3a-ol) and 7-dehydrocholesterol (cholsta-5,7-dien-3,8-ol) from Schwartz/Mann; bovine serum albumin from Pentex Biochemical; cytochrome c from Mann Research Laboratories; p-hydroxymercuribenzoate (pHMB) from Sigma Chemical Co.; oxidized dithiothreitol (DTT) from Calbiochem; and reduced DTT from Koch Light Laboratories. Purification of cytolysins. Pneumolysin was purified and titrated as described previously (11, 12

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“…HepG2 cells were permeabilized by a two-step procedure using the bacterial toxin Strep O, which binds tightly to the plasma membrane in a temperature-independent way, and produces pores of uniform size in a temperature-dependent fashion by complexation with cholesterol (Buckingham and Duncan, 1983;Duncan and Schlegel, 1975). Pore formation is considered irreversible (Kanbayashi et al, 1972;Duncan and Schlegel, 1975). Binding of Strep O was carried out at 0°C and unbound Strep O was removed by washing .…”

Section: Permeabilization Ofhepg2 Cellsmentioning

confidence: 99%

Retrograde transport from the Golgi region to the endoplasmic reticulum is sensitive to GTP gamma S.

Tan

Bolscher

Feltkamp

et al. 1992

The Journal of Cell Biology

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Abstract. The involvement of GTP-binding proteins in the intracellular transport of the secretory glycoprotein a,-antitrypsin was investigated in streptolysin O-permeabilized HepG2 cells. This permeabilization procedure allows ready access to the intracellular milieu of the membrane-impermeant, nonhydrolyzable GTP analog GTPyS. In streptolysin O-permeabilized HepG2 cells, the constitutive secretory pathway remains func-

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Kinetic Study on Streptolysin O*

Cited by 18 publications

References 0 publications

Evidence for a one-hit theory in the immune bactericidal reaction and demonstration of a multi-hit response for hemolysis by streptolysin O and Clostridium perfringens theta-toxin

Evidence for a one-hit theory in the immune bactericidal reaction and demonstration of a multi-hit response for hemolysis by streptolysin O and Clostridium perfringens theta-toxin

Binding of cholesterol by sulfhydryl-activated cytolysins

Retrograde transport from the Golgi region to the endoplasmic reticulum is sensitive to GTP gamma S.

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