A B S T R A C TThe kinetics of the urease-catalysed hydrolysis of urea in phosphate and citrate buffers have been studied. The effects of urease concentration, substrate concentrution, p H and temperature of solutions, and particularly the concentrations of bu#ers and product on the reaction rate of urea hydrolysis have been examined. The activation energies in two buffer systems indicate that the reaction mechanism catalysed by urease may be the same in each case. A substrate inhibition model has been develo…
scite is a Brooklyn-based startup that helps researchers better discover and evaluate scientific articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by researchers from dozens of countries and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.