volume 52, issue 4, P433-444 2007
DOI: 10.1002/jctb.280520402
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Abstract: A B S T R A C TThe kinetics of the urease-catalysed hydrolysis of urea in phosphate and citrate buffers have been studied. The effects of urease concentration, substrate concentrution, p H and temperature of solutions, and particularly the concentrations of bu#ers and product on the reaction rate of urea hydrolysis have been examined. The activation energies in two buffer systems indicate that the reaction mechanism catalysed by urease may be the same in each case. A substrate inhibition model has been develo…

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