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Cited by 31 publications
(30 citation statements)
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References 9 publications
(13 reference statements)
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“…The detection of thermodynamically nonequivalent F binding sites in the quaternary complex is the first such evidence for nonequivalent ligand binding to enolase. Nonequivalent binding sites for cations, however, have been observed (Hanlon & Westhead, 1969).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…The detection of thermodynamically nonequivalent F binding sites in the quaternary complex is the first such evidence for nonequivalent ligand binding to enolase. Nonequivalent binding sites for cations, however, have been observed (Hanlon & Westhead, 1969).…”
Section: Discussionmentioning
confidence: 99%
“…Direct metal ion binding studies to enolase and enolasecontaining substrate (Hanlon & Westhead, 1969;Faller et al, 1977;Faller t Johnson, 1974) The simplest model proposed to explain the PRR data is the displacement of one of the two water molecules coordinated to enolase-Mn (Nowak et al, 1973) by F. The Pi can interact with the bound Mn2+ in a second sphere complex via hydrogen bonding through a water molecule. This structure is analogous to the interaction of the bound Mn2+ with the phosphoryl group of the substrate demonstrated by high-resolution NMR studies of the ternary enzyme-Mnsubstrate complex (Nowak et al, 1973).…”
Section: Discussionmentioning
confidence: 99%
“…Slight differences between the conditions used to measure them and those employed in this study were shown experimentally to be immaterial. The two remaining constants ( K , and K P ) can be calculated from the Michaelis and velocity constants for the dehydration and hydration reactions as described by Hanlon and Westhead (1969b). Significantly different experimental conditions were used to obtain the published values, so the steady-state parameters for the enolase reaction had to be reevaluated.…”
Section: Ka5mentioning
confidence: 99%
“…10 Metal binding in site I, traditionally called "conformational", induces a conformational change in the enzyme and enables binding of substrate or substrate analogues. 11,12 Following binding of a substrate or a substrate analogue, the second metal ion, called "catalytic", can bind 13,14 in site II and then the catalytic reaction occurs. 10 Release of the ligands is more complex.…”
Section: Introductionmentioning
confidence: 99%