Kinetic studies of C. pyrenoidosa using 94% 13C CO2

Fowler, E.B.; Adams, William; Christenson, C.W.; Kollman, V.H.; Buchholz, Jens

doi:10.1002/bit.260140511

Cited by 15 publications

(14 citation statements)

References 8 publications

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“…It has also been established that 40% of brain hydrogen but only 10% of fatty acid hydrogen is derived from body water (for most tissues this figure is 20-30%). (51) In some reactions where radical or ion-radical intermediate state is involved, nuclear spin-selective processes (the magnetic IE (19) ) can play an important role; for example, phosphorylation reactions (52) can have 2.6-fold higher yields when nuclear spin-possessing 25 Mg 2þ ions are used instead of the spinless 24 Mg isotope. (53) Regrettably there are currently no data on isotope discrimination by living organisms based on this intriguing variety of the IE.…”

Section: Seamentioning

confidence: 99%

“…Neither inhibition nor a requirement for adaptation is encountered when alga is grown on 13 CO 2 , with no observable morphological changes. (24) High concentrations of D 2 O are known to cause some abnormalities, but the simultaneous presence of 13 C can diminish this, at least in alga. (21,25) The toxicity of 13 C-labeled markers, as potential replacements for radioactive labeling, was tested on mice.…”

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confidence: 98%

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Do “heavy” eaters live longer?

Shchepinov¹

2007

BioEssays

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A new hypothesis is put forward, linking cellular endurance with dietary consumption of stable heavy isotopes. Due to the isotope effect, biomolecules that incorporate heavier isotopes give rise to more stable molecular structures with increased resistance to damages associated with aging and age-related disease. The inclusion of heavy isotopes might be either active (selection for heavier isotopes) or passive (incorporation reflecting the existing abundance). The hypothesis links consumption of foods relatively rich in heavy isotopes (such as (13)C and D, derived from C4-plants), especially at the early stages of the organism's development, with enhanced longevity. Implications of diets and intestinal microflora are also discussed.

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Section: Seamentioning

confidence: 99%

mentioning

confidence: 98%

Do “heavy” eaters live longer?

Shchepinov¹

2007

BioEssays

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“…In biological systems, each of the four most abundant elements (C, H, O and N) has more than one stable isotope, the lighter one being the most abundant (e.g., the share of 12 C is 98.9%). Microbes, plants and animals, including mammals, can grow in an environment with signi cantly altered ratios of stable isotopes compared to natural, but the phenotype of the organisms can be signi cantly affected [7][8][9][10][11][12][13][14] . The researchers concluded that the "organisms of different isotopic compositions are actually different organisms, to the degree that their isotopic compositions are removed from naturally occurring compositions" 10 .…”

Section: Introductionmentioning

confidence: 99%

Ultralight ultrafast enzymes

Zhang

Meng

Beusch

et al. 2021

Preprint

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Inorganic materials depleted of heavy stable isotopes are known to deviate strongly in some physico-chemical properties from their isotopically natural (native) counterparts; however, in biotechnology such effects have not been investigated yet. Here we explored for the first time the effect of simultaneous depletion of the heavy carbon, hydrogen, oxygen and nitrogen isotopes on the bacterium E. coli and the enzymes expressed in it. Bacteria showed faster growth, with proteins exhibiting higher thermal stability, while for recombinant enzymes expressed in ultralight media, faster kinetics was discovered. At room temperature, luciferase, thioredoxin and dihydrofolate reductase showed a 40-250% increase in activity compared to the native counterparts. The efficiency of ultralight Pfu DNA polymerase in polymerase chain reaction was also significantly higher than that of the normal enzyme. At 10 °C, the advantage factor of ultralight enzymes typically increased by 50%, which points towards the reduction in structural entropy as the main factor explaining the kinetic effect of heavy isotope depletion. Ultralight enzymes may find an application where extreme reaction rates are required.

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“…After a period of adaptation (“lag phase”), growth resumes, but the rate is usually slower than in normal isotopic environment [8] . The changes in the growth rate can be explained by the impact of isotopic substitutions on the kinetics of enzymes [9] , pattern of hydrogen bonds and similar relatively subtle but cumulatively potentially important effects.…”

Section: Introductionmentioning

confidence: 99%

Effects of Low-Level Deuterium Enrichment on Bacterial Growth

Xie

Zubarev

2014

PLoS ONE

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Using very precise (±0.05%) measurements of the growth parameters for bacteria E. coli grown on minimal media, we aimed to determine the lowest deuterium concentration at which the adverse effects that are prominent at higher enrichments start to become noticeable. Such a threshold was found at 0.5% D, a surprisingly high value, while the ultralow deuterium concentrations (≤0.25% D) showed signs of the opposite trend. Bacterial adaptation for 400 generations in isotopically different environment confirmed preference for ultralow (≤0.25% D) enrichment. This effect appears to be similar to those described in sporadic but multiple earlier reports. Possible explanations include hormesis and isotopic resonance phenomena, with the latter explanation being favored.

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Kinetic studies of C. pyrenoidosa using 94% ¹³C CO₂

Cited by 15 publications

References 8 publications

Do “heavy” eaters live longer?

Do “heavy” eaters live longer?

Ultralight ultrafast enzymes

Effects of Low-Level Deuterium Enrichment on Bacterial Growth

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