Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2012.03.007 View full text
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Fabrice Gritti, Georges Guiochon

Abstract: The kinetic performance of 0.5 mm × 50 mm columns packed with 2.7 μm Halo-C(18) core-shell particles and 3 μm EP-120-C(18) fully porous particles fitted on an Eksigent LC-Express Ultra μHPLC system were measured. The instrument contribution to band broadening was obtained by directly connecting the injection valve and the detector cell with a short, narrow PEEKSIL tube. The connections between the column and the connecting tubes, the column endfittings and its frits contribute to band spreading and are respons…

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