Kinetic dynamics in heterogeneous enzymatic hydrolysis of cellulose: an overview, an experimental study and mathematical modelling

Kinetics of Cellulose Hydrolysis in an Oscillatory Baffled Reactor

“…However, as the ASR was increased to 70 s -1 (corresponding to 450 rpm) the model showed a significant drop in the saccharification yields and a significant reduction even in the initial rates as shown in the figure. This is contrary to the observation of Gan et al (2003) that the reducing sugar yield is only modestly dependent on the agitation speed and that the absolute shear stress may not be sufficiently high enough to cause any significant damage to the enzyme. Although, deactivation was not measured directly in this work, but clearly, the enzyme activity seemed to have declined appreciably in this case.…”

Kinetics of Cellulose Hydrolysis in an Oscillatory Baffled Reactor

“…However, as the ASR was increased to 70 s -1 (corresponding to 450 rpm) the model showed a significant drop in the saccharification yields and a significant reduction even in the initial rates as shown in the figure. This is contrary to the observation of Gan et al (2003) that the reducing sugar yield is only modestly dependent on the agitation speed and that the absolute shear stress may not be sufficiently high enough to cause any significant damage to the enzyme. Although, deactivation was not measured directly in this work, but clearly, the enzyme activity seemed to have declined appreciably in this case.…”

The effects of α‐L‐rhamnosidase, β‐D‐glucosidase, and their combination on the quality of orange juice

“…Figure 1b shows that the polyphenols content in RGOJ, ROJ, and GOJ were not significantly different, and the polyphenols content in RGOJ was lower than the sum of those in ROJ and GOJ. This result might be attributed to the substrate competition and inhibition between α-L-rhamnosidase and β-D-glucosidase, which was similar to the substrate competition and inhibition pattern of previous studies (Gan et al, 2003;Zhang et al, 2017).…”

Enzymatic Recycling of High-Value Phosphor Flame-Retardant Pigment and Glucose from Rayon Fibers

“…Interestingly, for both conditions (25 and 50 °C) the highest yield of RS was not achieved with the highest enzyme loading which could be due to saturation of the binding sites on the rayon fibers as previously reported for cellulose; moreover, it is well-known that competitive inhibition between exoglucanases and endoglucanases under saturating conditions occurs. − This trend observed for the RS was confirmed by quantification of glucose released via the HPLC analysis (Figure ). In general, a similar overall yield can be achieved at 8 h of incubation at 50 °C and 24 h at 25 °C at an enzyme loading of 5 × 10 3 FPU/g Rf allowing energy savings for heating at longer incubation times.…”

Kinetics of Cellulose Hydrolysis in an Oscillatory Baffled Reactor

“…However, as the ASR was increased to 70 s -1 (corresponding to 450 rpm) the model showed a significant drop in the saccharification yields and a significant reduction even in the initial rates as shown in the figure. This is contrary to the observation of Gan et al (2003) that the reducing sugar yield is only modestly dependent on the agitation speed and that the absolute shear stress may not be sufficiently high enough to cause any significant damage to the enzyme. Although, deactivation was not measured directly in this work, but clearly, the enzyme activity seemed to have declined appreciably in this case.…”

The effects of α‐L‐rhamnosidase, β‐D‐glucosidase, and their combination on the quality of orange juice

“…Figure 1b shows that the polyphenols content in RGOJ, ROJ, and GOJ were not significantly different, and the polyphenols content in RGOJ was lower than the sum of those in ROJ and GOJ. This result might be attributed to the substrate competition and inhibition between α-L-rhamnosidase and β-D-glucosidase, which was similar to the substrate competition and inhibition pattern of previous studies (Gan et al, 2003;Zhang et al, 2017).…”

Enzymatic Recycling of High-Value Phosphor Flame-Retardant Pigment and Glucose from Rayon Fibers

“…Interestingly, for both conditions (25 and 50 °C) the highest yield of RS was not achieved with the highest enzyme loading which could be due to saturation of the binding sites on the rayon fibers as previously reported for cellulose; moreover, it is well-known that competitive inhibition between exoglucanases and endoglucanases under saturating conditions occurs. − This trend observed for the RS was confirmed by quantification of glucose released via the HPLC analysis (Figure ). In general, a similar overall yield can be achieved at 8 h of incubation at 50 °C and 24 h at 25 °C at an enzyme loading of 5 × 10 3 FPU/g Rf allowing energy savings for heating at longer incubation times.…”

Kinetics of Cellulose Hydrolysis in an Oscillatory Baffled Reactor

“…However, as the ASR was increased to 70 s -1 (corresponding to 450 rpm) the model showed a significant drop in the saccharification yields and a significant reduction even in the initial rates as shown in the figure. This is contrary to the observation of Gan et al (2003) that the reducing sugar yield is only modestly dependent on the agitation speed and that the absolute shear stress may not be sufficiently high enough to cause any significant damage to the enzyme. Although, deactivation was not measured directly in this work, but clearly, the enzyme activity seemed to have declined appreciably in this case.…”

The effects of α‐L‐rhamnosidase, β‐D‐glucosidase, and their combination on the quality of orange juice

“…Figure 1b shows that the polyphenols content in RGOJ, ROJ, and GOJ were not significantly different, and the polyphenols content in RGOJ was lower than the sum of those in ROJ and GOJ. This result might be attributed to the substrate competition and inhibition between α-L-rhamnosidase and β-D-glucosidase, which was similar to the substrate competition and inhibition pattern of previous studies (Gan et al, 2003;Zhang et al, 2017).…”

Enzymatic Recycling of High-Value Phosphor Flame-Retardant Pigment and Glucose from Rayon Fibers

“…Interestingly, for both conditions (25 and 50 °C) the highest yield of RS was not achieved with the highest enzyme loading which could be due to saturation of the binding sites on the rayon fibers as previously reported for cellulose; moreover, it is well-known that competitive inhibition between exoglucanases and endoglucanases under saturating conditions occurs. − This trend observed for the RS was confirmed by quantification of glucose released via the HPLC analysis (Figure ). In general, a similar overall yield can be achieved at 8 h of incubation at 50 °C and 24 h at 25 °C at an enzyme loading of 5 × 10 3 FPU/g Rf allowing energy savings for heating at longer incubation times.…”