Kinetic and thermodynamic parameters of β-glucosidase immobilized on various colloidal particles from a paddy soil

Yan, Jinlong; Pan, Genxing; Ding, Cheng; Quan, Guixiang

doi:10.1016/j.colsurfb.2010.04.015

Cited by 35 publications

(33 citation statements)

References 23 publications

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“…The smaller the ΔH # -value, the lower the amounts of energy needed for elongation and compression of the chemical bonds to attain the transition state or the activated complex [29]. Moreover, positive sign of the ΔH # -value indicated endothermic nature of the present reaction system [29,32,33]. Thus, lower ΔH # value in MTBE solvent was attributed to ease of formation of enzyme-substrate complex because of the more flexible enzyme conformation in a hydrophobic etheric solvent [34].…”

Section: Thermo-kinetic Energy Assessment For Synthesis Of Hexyl Levumentioning

confidence: 73%

“…6, purple line). The drop in % yield at each cycle was attributed to handling loss and deactivation of lipase by continuous exposure to hexanol, since alcoholic substrate acts as an inhibitor to enzyme activity [17,18,22,[29][30][31]. Conversely, % yield of the hexyl levulinate synthesis in each cycle could also be enhanced by increasing reaction time span from 8 h to 10 h (Fig.…”

Section: Catalyst Recyclabilitymentioning

confidence: 85%

“…The lower enthalpy of activation (ΔH # -value) indicated easy formation of the enzyme-substrate complex, while higher ΔH # -value indicated complexity in the enzyme-substrate complex formation [29,32,33]. The smaller the ΔH # -value, the lower the amounts of energy needed for elongation and compression of the chemical bonds to attain the transition state or the activated complex [29]. Moreover, positive sign of the ΔH # -value indicated endothermic nature of the present reaction system [29,32,33].…”

Section: Thermo-kinetic Energy Assessment For Synthesis Of Hexyl Levumentioning

confidence: 99%

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Thermo-chemical energy assessment for production of energy-rich fuel additive compounds by using levulinic acid and immobilized lipase

Badgujar

Bhanage

2015

Fuel Processing Technology

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Section: Thermo-kinetic Energy Assessment For Synthesis Of Hexyl Levumentioning

confidence: 73%

Section: Catalyst Recyclabilitymentioning

confidence: 85%

Section: Thermo-kinetic Energy Assessment For Synthesis Of Hexyl Levumentioning

confidence: 99%

See 1 more Smart Citation

Thermo-chemical energy assessment for production of energy-rich fuel additive compounds by using levulinic acid and immobilized lipase

Badgujar

Bhanage

2015

Fuel Processing Technology

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“…In the original soil ('cero' OM removal), potentially active proteins may be blocked by the presence of humic matter, being not accessible to be extracted by the buffer solutions used. This is supported by the fact that humic matter has shown to exert an inhibitory effect on soil enzyme activity (Yan et al, 2010) that is explained by different mechanisms, including complexation of active sites leading to a conformational change of the enzyme, competition with the substrate for the catalytically active site, and/or binding of the substrate to humic acids (Ruggiero et al, 1996). Recently, Mazzei et al (2013) demonstrated, using direct measurements by 1 H-NMR spectroscopy, that humic supramolecules are able to form weakly-bond complexes with extracellular enzymes (alkaline phosphatase) that show reduced catalytic activity.…”

Section: Effect Organic Matter Removalmentioning

confidence: 78%

Efficiency of three buffers for extracting β-glucosidase enzyme in different soil orders: Evaluating the role of soil organic matter

Gutiérrez¹,

Blu²,

Molina-Roco³

et al. 2017

Sci.agropec

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The objective of this research was to evaluate extraction methods for β-glucosidases comparing three buffer solutions (MUB, acetate, and maleate) at different incubation times (0.5 h to 10 h) and in three different soil orders (Mollisols, Andisols and Ultisols). Seven acidic soils were evaluated, showing differences in pH, OM, and clay contents. To evaluate the effect of OM as enzymes source, one soil of each order was treated to partially remove its OM and then the enzyme assay was performed. When using MUB and maleate buffers the highest (32 and 31 µg-pNP g-soil -1 h -1 in average, respectively) were found, and the latter was significantly (p < 0.050) correlated with the soil clay content. The activity obtained with acetate buffer was much lower (38.2 µg-pNP g-soil -1 h -1 in average). The use of MUB buffer with 1 h of incubation is suggested as extraction method, showing good reproducibility and allowing to express higher enzyme potential for soil comparisons. For the Andisol and Ultisol, the enzyme activity significantly decreased with the OM removal (%) indicating that OM is the major source of the measured β-glucosidase activity, while a different trend was observed for the Mollisol, in which the mineral fraction (mainly 2:1 type clay) appears to be involved in the increased enzyme activity displayed after the initial OM removal.

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“…where Qe (mg g -1 ) is the amount of pymetrozine adsorbed onto the BC from the solution at equilibrium, Ce (mg L -1 ) is the equilibrium concentration of pymetrozine in the solution, R is the gas constant (8.314 (Liu et al 2009;Yan et al 2010).…”

Section: Batch Adsorption Experimentsmentioning

confidence: 99%

Removal of the Pesticide Pymetrozine from Aqueous Solution by Biochar Produced from Brewer's Spent Grain at Different Pyrolytic Temperatures

Xi¹,

Yan²,

Quan³

et al. 2014

BioResources

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Biochar (BC) produced from brewer's spent grain (BSG) via slow pyrolysis at 300, 400, 500, 600, and 700 °C was characterized and investigated as an adsorbent for the removal of the pesticide pymetrozine from aqueous solution. Batch BSG BCs adsorption experiments were carried out under various conditions (such as pH, pymetrozine concentration, and BC dosage) to adsorb the pymetrozine. The BSG BCs adsorption pymetrozine capacities were increased by 21.4% to 55.5% under pyrolysis temperatures of 300, 400, 500, and 600 °C compared to 700 °C with a pyrolysis time of 2 h and by 19.0% to 52.1% at 4 h. At solution pH values of 2, 4, 6, and 8, the adsorption capacities were increased by 9.6% to 39.5% compared with that at pH 10. A similar adsorption tendency was found for the different BCs dosage. In the first 60 min, BC absorbed 70% to 80% pymetrozine. The Langmuir and Freundlich model were highly correlated with BC adsorption. The magnitude of free energy decreased by 32.2% to 47.3% with increasing temperature. The value of the enthalpy change showed the adsorption to be endothermic. The BSG BC had high efficiency in adsorbing pymetrozine and had great potential to prevent the water pollution and reuse the waste of the beer factory.

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Kinetic and thermodynamic parameters of β-glucosidase immobilized on various colloidal particles from a paddy soil

Cited by 35 publications

References 23 publications

Thermo-chemical energy assessment for production of energy-rich fuel additive compounds by using levulinic acid and immobilized lipase

Thermo-chemical energy assessment for production of energy-rich fuel additive compounds by using levulinic acid and immobilized lipase

Efficiency of three buffers for extracting β-glucosidase enzyme in different soil orders: Evaluating the role of soil organic matter

Removal of the Pesticide Pymetrozine from Aqueous Solution by Biochar Produced from Brewer's Spent Grain at Different Pyrolytic Temperatures

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