KIN10 promotes stomatal development through stabilization of the SPEECHLESS transcription factor

Han, Chao; Liu, Yue; Su, Wen Pang; Qiao, Yan; Wang, Lingyan; Tian, Yanchen; Fan, Min; Deng, Zhiping; Lau, On Sun; Jaeger, Geert De; Bai, Ming-Yi

doi:10.1038/s41467-020-18048-w

Cited by 57 publications

(70 citation statements)

References 43 publications

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“…Introduction of 2% glucose to growth media increased the stem-cell like ACDs even in ethylene insensitive mutants indicating that the epidermis is likely perceiving these signals independently. An effect of sugars on stomatal production was recently reported (Han et al, 2020) and while their experimental conditions differ substantially from ours, both studies concur that sugars promote stomatal development. What information is sugar providing?…”

Section: Discussionsupporting

confidence: 84%

Tuning of self-renewing capacity in theArabidopsisstomatal lineage by hormonal and nutrition regulation of asymmetric cell divisions

Gong

Alassimone

Varnau

et al. 2020

Preprint

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Asymmetric and self-renewing divisions build and pattern tissues. In the Arabidopsis thaliana stomatal lineage, asymmetric cell divisions, guided by polarly localized cortical proteins, generate the majority of cells on the leaf surface. These divisions can be fine-tuned by systemic and environmental signals to modify tissue development, but the molecular mechanisms by which plants incorporate such cues to regulate asymmetric divisions are largely unknown. In a screen for modulators of cell polarity and asymmetric divisions, we identified a mutation in CONSTITIUTIVE TRIPLE RESPONSE 1, a negative regulator of ethylene signaling. We subsequently revealed antagonistic impacts of ethylene and glucose signaling on the self-renewing capacity of stomatal lineage stem cells. Quantitative analysis of the impacts of these signaling systems on cell polarity and fate dynamics showed that developmental information may be encoded in both the spatial and temporal asymmetries of polarity proteins. Taken together, our results provide a framework for a mechanistic understanding of how systemic information such as nutritional status and environmental factors tune stem cell behavior in the stomatal lineage, ultimately enabling optimization of leaf size and cell-type composition.

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Section: Discussionsupporting

confidence: 84%

Tuning of self-renewing capacity in theArabidopsisstomatal lineage by hormonal and nutrition regulation of asymmetric cell divisions

Gong

Alassimone

Varnau

et al. 2020

Preprint

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“…Recent applications of scRNA-seq to plants include detailed analyses of regeneration and meiosis utilizing hundreds of highly selected cells (Efroni et al, 2016; Nelms and Walbot, 2019). Conversely, atlases comprised of tens of thousands of cells primarily focus on the Arabidopsis thaliana (herein referred to as Arabidopsis) root, and they provide refined insight to well-characterized cell type-specific lineage progressions (Denyer et al, 2019; Ryu et al, 2019; Shahan et al, 2020; Shulse et al, 2019; Zhang et al, 2019). While these pioneering studies have validated scRNA-seq approaches for resolving unidirectional differentiation processes, they have not focused on flexible and indeterminate features of plant development.…”

Section: Introductionmentioning

confidence: 99%

Single-Cell Resolution of Lineage Trajectories in the Arabidopsis Stomatal Lineage and Developing Leaf

Lopez‐Anido

Vatén

Smoot

et al. 2020

Preprint

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SUMMARYDynamic cell states underlie flexible developmental programs, such as with the stomatal lineage of the Arabidopsis epidermis. Initial stages of the lineage feature asynchronous and indeterminate divisions modulated by environmental cues, enabling cell fate flexibility to generate the requisite density and pattern of stomata for a given environment. It remains unclear, however, how flexibility of cell fates is controlled. Here, we uncovered distinct models of cell state differentiation within Arabidopsis leaf tissue by leveraging single-cell transcriptomics and molecular genetics. Our findings resolved underlying heterogeneity within cell states of the flexible epidermal stomatal lineage, which appear to exist along a continuum, with progressive cell specification. Beyond the early stages of the lineage, we discovered that the core transcriptional regulator SPEECHLESS is required for cell fate commitment to yield stomatal guard cells. Overall, our work has refined the stomatal lineage paradigm and uncovered progressive cell state decisions along lineage trajectories in developing leaves.

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“…Energy hemostasis during stomatal development is controlled by sucrose nonfermenting-1 (SNF1)-related kinase 1 (SnRK1), which is a central energy sensor kinase in plants [71]. In Arabidopsis, SnRK1 functions as heterotrimeric complexes composed of one α-catalytic subunit (KIN10, KIN11, and KIN12) and two regulatory subunits, β and γ [72,73]. Sucrose-induced KIN10 expresses in all epidermal cells but exhibits cell-specific subcellular localization, accumulation in liquid culture conditions [73].…”

Section: Highly Conserved Snrk1 Positively Regulates Spch-mediated Stmentioning

confidence: 99%

SPEECHLESS and MUTE Mediate Feedback Regulation of Signal Transduction during Stomatal Development

Wakeel

Wang

2021

Plants

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Stomatal density, spacing, and patterning greatly influence the efficiency of gas exchange, photosynthesis, and water economy. They are regulated by a complex of extracellular and intracellular factors through the signaling pathways. After binding the extracellular epidermal patterning factor 1 (EPF1) and 2 (EPF2) as ligands, the receptor-ligand complexes activate by phosphorylation through the MAP-kinase cascades, regulating basic helix-loop-helix (bHLH) transcription factors SPEECHLESS (SPCH), MUTE, and FAMA. In this review, we summarize the molecular mechanisms and signal transduction pathways running within the transition of the protodermal cell into a pair of guard cells with a space (aperture) between them, called a stoma, comprising asymmetric and symmetric cell divisions and draw several functional models. The feedback mechanisms involving the bHLH factors SPCH and MUTE are not fully recognized yet. We show the feedback mechanisms driven by SPCH and MUTE in the regulation of EPF2 and the ERECTA family. Intersections of the molecular mechanisms for fate determination of stomatal lineage cells with the role of core cell cycle-related genes and stabilization of SPCH and MUTE are also reported.

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KIN10 promotes stomatal development through stabilization of the SPEECHLESS transcription factor

Cited by 57 publications

References 43 publications

Tuning of self-renewing capacity in theArabidopsisstomatal lineage by hormonal and nutrition regulation of asymmetric cell divisions

Tuning of self-renewing capacity in theArabidopsisstomatal lineage by hormonal and nutrition regulation of asymmetric cell divisions

Single-Cell Resolution of Lineage Trajectories in the Arabidopsis Stomatal Lineage and Developing Leaf

SPEECHLESS and MUTE Mediate Feedback Regulation of Signal Transduction during Stomatal Development

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