Key signalling factors and pathways in the molecular determination of skeletal muscle phenotype

Chang, Kin-Chow

doi:10.1017/s1751731107702070

Cited by 33 publications

(29 citation statements)

References 186 publications

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“…In animal production for meat, meat color and tenderness are closely associated with a predominance of red fibers (Chang, 2007). This study showed that porcine soleus muscle is redder than EDL muscle ( Figure 1A, B), and increased oxidative type I fibers in porcine soleus muscle were shown by immunohistochemical staining ( Figure 1C), in agreement with an earlier report (Lefaucheur et al, 2004).…”

Section: Muscle Fiber Composition In Porcine Soleus and Edl Musclessupporting

confidence: 91%

Correlation of forkhead box transcription factor O1 and myosin heavy chain isoforms in porcine skeletal muscle

et al. 2014

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ABSTRACT. We examined the expression of myosin heavy chain (MyHC) isoforms and forkhead box transcription factor O1 (FoxO1) in porcine soleus and extensor digitorum longus (EDL) muscles to clarify the correlation of FoxO1 and the relative abundance of transcripts of MyHC isoforms. Soleus muscle was found to be redder than EDL muscles in pigs, and immunohistochemical fast MyHC staining showed more oxidative type I fibers compared to EDL. qRT-PCR quantification of MyHC isoforms I, IIa, IIx, and IIb showed that expression of MyHC I and MyHC IIa mRNAs was much higher, whereas expression of MyHC IIx and MyHC IIb mRNAs was much lower in porcine soleus muscle compared to EDL muscle. Expression of FoxO1 mRNA and p-FoxO1 protein was significantly more abundant in porcine soleus muscle compared to EDL muscle. The expression of phosphorylated FoxO1 (p-FoxO1) was positively correlated with the expression of MyHC I (R = 0.9747, P < 0.01) and negatively correlated with the expression of MyHC IIx (R = -0.9963, P < 0.01) and MyHC IIb (R = -0.9834, P < 0.01). Taken together, these results suggested that FoxO1 may play a pivotal role in the determination of muscle fiber type.

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Section: Muscle Fiber Composition In Porcine Soleus and Edl Musclessupporting

confidence: 91%

Correlation of forkhead box transcription factor O1 and myosin heavy chain isoforms in porcine skeletal muscle

et al. 2014

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“…In search of potential downstream effectors of H19/let-7-mediated regulation during muscle differentiation, we focused our attention on insulin-like growth factors (IGF1 and IGF2), major regulators of pre- and postnatal muscle development and growth (Braun and Gautel, 2011; Chang, 2007). IGF1 and IGF2 act through the same receptor, IGF1R, to promote myogenesis (Braun and Gautel, 2011; Chang, 2007).…”

Section: Resultsmentioning

confidence: 99%

The Imprinted H19 LncRNA Antagonizes Let-7 MicroRNAs

et al. 2013

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SUMMARY Abundantly expressed in fetal tissues and adult muscle, the developmentally regulated H19 long noncoding RNA (lncRNA) has been implicated in human genetic disorders and cancer. However, how H19 acts to regulate gene function has remained enigmatic, despite the recent implication of its encoded miR675 in limiting placental growth. We noted that vertebrate H19 harbors both canonical and noncanonical binding sites for the let-7 family of microRNAs, which plays important roles in development, cancer, and metabolism. Using H19 knockdown and overexpression, combined with in vivo crosslinking and genome-wide transcriptome analysis, we demonstrate that H19 modulates let-7 availability by acting as a molecular sponge. The physiological significance of this interaction is highlighted in culture where H19 depletion causes precocious muscle differentiation, a phenotype recapitulated by let-7 overexpression. Our results reveal an unexpected mode of action of H19 and identify this lncRNA as an important regulator of the major let-7 family of microRNAs.

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“…Narkar et al (2008) found that peroxisome proliferator-activated receptor d (PPARd) agonist can increase oxidative myofibres in adult mice, and CLA can act as a ligand for some of PPARs family, such as PPARa, g and d (Meadus et al, 2002;Bassaganya-Riera et al, 2004). Furthermore, Chang (2007) reported that PGC-1a activation, in partnership with nuclear hormone receptors, including PPARs, could increase oxidative capacity in skeletal muscle. These reports would provide some basis for further studies on the specific mechanism of CLA influencing muscle-fibre types.…”

Section: Discussionmentioning

confidence: 99%

Age-related changes and nutritional regulation of myosin heavy-chain composition in longissimus dorsi of commercial pigs

Men

Deng

et al. 2013

Animal

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The objective of this study is to investigate the age-related changes of and the effects of dietary conjugated linoleic acid (CLA) on muscle-fibre types in commercial pigs. We divided 25 crossbred male pigs into five age groups (7, 30, 60, 100 and 180 days) and 30 finishing pigs into two dietary groups (one fed a CLA-enriched diet and the other fed a control diet for 30 days). We analysed the composition (%) of myosin heavy-chain (MyHC) mRNA according to the absolute copies of each MyHC (I, IIa, IIb and IIx) mRNA, and the activities of succinate dehydrogenase (SDH) and malate dehydrogenase (MDH) in the longissimus muscle. From days 7 to 180, the MyHC I mRNA abundance and SDH and MDH activities presented a decreasing trend, the MyHC IIb mRNA abundance presented a steady trend and the MyHC IIa and IIx mRNA abundances presented an increasing trend. On day 30, MyHC I and IIb mRNA abundances were at their lowest (P , 0.05), and the MyHC IIa and IIx mRNA abundances were at their highest (P , 0.05). In the CLA group, the MyHC I mRNA abundance and the activities of SDH and MDH were improved in the longissimus muscle, whereas pressure loss, drip loss and average back fat depth significantly decreased (P , 0.01) and shear force significantly increased (P , 0.01). Loin eye area, feed conversion rate and meat colour showed some tendency to be improved. These results indicated that more oxidative fibres might convert to glycolytic fibres with increasing age or weight, and that the early developmental stage might be a key stage for this conversion. During the finishing stage, the proportion of oxidative fibres might be increased by dietary CLA supplementation, which may contribute to the water-holding capacity of meat. The results would provide an important basis for the application of muscle-fibre types in the improvement of pork quality.

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Key signalling factors and pathways in the molecular determination of skeletal muscle phenotype

Cited by 33 publications

References 186 publications

Correlation of forkhead box transcription factor O1 and myosin heavy chain isoforms in porcine skeletal muscle

Correlation of forkhead box transcription factor O1 and myosin heavy chain isoforms in porcine skeletal muscle

The Imprinted H19 LncRNA Antagonizes Let-7 MicroRNAs

Age-related changes and nutritional regulation of myosin heavy-chain composition in longissimus dorsi of commercial pigs

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