Key proteins of proteome underlying sperm malformation of rats exposed to low fenvalerate doses are highly related to <scp>P53</scp>

Huang, Shaoping; Lu, Ying; Li, Suying; Zhou, Tao; Wang, Jing; Xia, Jiangyan; Zhang, Xinxin; Zhou, Zuomin

doi:10.1002/tox.23117

Cited by 5 publications

(3 citation statements)

References 66 publications

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“…Spermatogenesis, the primary testicular function, is a complex morphological change of germ cell differentiation that involves self-renewal and differentiation of spermatogonia, meiosis of spermatocytes and spermiogenesis ( Huang et al, 2021 ). Alteration of any stage of spermiogenesis will damage sperm quality and ultimately impact male fertility.…”

Section: Discussionmentioning

confidence: 99%

The Abscopal Effects of Cranial Irradiation Induce Testicular Damage in Mice

et al. 2021

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To investigate whether the abscopal effects of cranial irradiation (C-irradiation) cause testicular damage in mice, male C57BL/6 mice (9weeks of age) were randomly divided into a sham irradiation group, a shielded group and a C-irradiation group and administered sham/shielded irradiation or C-irradiation at a dose rate of 2.33Gy/min (5Gy/d for 4 d consecutively). All mice were sacrificed at 4weeks after C-irradiation. We calculated the testis index, observed testicular histology by haematoxylin-eosin (HE) staining and observed testicular ultrastructure by transmission electron microscopy. Western blotting was used to determine the protein levels of Bax, Bcl-2, Cleaved caspase 3, glial cell line-derived neurotrophic factor (GDNF) and stem cell factor (SCF) in the testes of mice. Immunofluorescence staining was performed to detect the expression of Cleaved caspase 3 and 3β hydroxysteroid dehydrogenase (3βHSD), and a TUNEL assay was used to confirm the location of apoptotic cells. The levels of testosterone (T), GDNF and SCF were measured by ELISA. We also evaluated the sperm quality in the cauda epididymides by measuring the sperm count, abnormality, survival rate and apoptosis rate. The results showed that there was no significant difference in testicular histology, ultrastructure or sperm quality between the shielded group and sham group. Compared with the sham/shielded group, the C-irradiation group exhibited a lower testis index and severely damaged testicular histology and ultrastructure at 4weeks after C-irradiation. The levels of apoptosis in the testes increased markedly in the C-irradiation group, especially in spermatogonial stem cells. The levels of serum T and testicular 3βHSD did not obviously differ between the sham group and the C-irradiation group, but the levels of GDNF and SCF in the testes increased in the C-irradiation group, compared with the sham group. In addition, the sperm count and survival rate decreased in the C-irradiation group, while the abnormality and apoptosis rate increased. Under these experimental conditions, the abscopal effects of C-irradiation induced testicular damage with regard to both structure and function and ultimately decreased sperm quality in mice. These findings provide novel insights into prevention and treatment targets for male reproductive damage induced by C-irradiation.

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Section: Discussionmentioning

confidence: 99%

The Abscopal Effects of Cranial Irradiation Induce Testicular Damage in Mice

et al. 2021

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“…Recent studies report that bifenthrin causes reduction in pregnancy potential and defects in fetal growth and development (Curtis et al, 2021; Park et al, 2021). Malformation of male gamates, DNA damage and epimutations in sperm and reproductive toxicity were evident in animal models that were exposed to fenvalerate, cypermethrin, permethrin, deltamethrin and fenpropathrin, respectively (Abu Zeid et al, 2021; Ha et al, 2021; Hozyen et al, 2020; Huang et al, 2021; Thorson et al, 2020). In most of the studies on pyrethroid toxicity, the effects were reported using a single compound.…”

Section: Introductionmentioning

confidence: 99%

A mixture of pyrethroids induces reduced fecundity and increased testicular genotoxicity in rats

2022

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To achieve crop protection and higher agricultural yield, pesticides are used; and among them pyrethroid based ones are the most preferred choice because of their specificity on the pests. Uncontrolled use of pesticides resulted in contamination of food products. Presence of residual amounts of pyrethroids was reported in agricultural products sold in Indian market, indicating that humans are constantly exposed to these chemicals through food on a routine basis. Studies that determine the toxic effects of pyrethroids at doses equivalent to human exposure are rare. We orally administered a mixture of pyrethroids (detected in the rice and vegetables of Indian market) to male rats for 15 months to mimic the long‐term exposure in humans. We observed reduced fecundity, sperm count and 13β‐hydroxysteroid dehydrogenase enzyme activity. The serum concentrations of hormones involved in male reproductive function were altered. Further, testicular genotoxicity as reflected by perturbations in the expression pattern of genes involved in the molecular processes of gametogenesis was evident. Such toxic effects may also be occurring in humans who consume agricultural products that contain residual amounts of pyrethroids on a regular basis throughout their lifetime.

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“…The nephro‐, hepato‐, and reproductive toxicity have been reported; besides the effects on general biochemical parameters of the blood. Bifenthrin induced toxicity in the liver and kidney of mice, [ 6 ] cypermethrin induced hepatotoxicity that involved ROS mediated crosstalk between Nrf2/Keap1 and NF‐κB/iκB‐α, [ 7 ] reduction in red and white blood cell counts and increased IL‐1b production in the liver of mice exposed to sub lethal dose of λ‐cyhalothrin, [ 8 ] induction of hyperthyroidism by cypermethirn via the PI3K/Akt‐FOXA1 cascade, [ 9 ] perturbations in the biochemical parameters of lung, liver, brain, heart and testis of rats by deltamethrin, [ 10 ] permethrin induced hepatotoxicity modulated by oxidative stress and mitochondrial dysfunction, [ 11 ] genotoxicity of fenpropathrin, [ 12 ] multi organ toxicity of pyrethroid based formulations, [ 13 ] liver fibrosis in quails by activation of TGF‐beta1/Smad pathway by Han et al, [ 14 ] nephrotoxicity of fenpropathrin in rats, [ 15 ] nephrotoxicity of rabbits by Anwar et al, [ 16 ] alterations in the sperm proteome of fenvalerate treated rats, [ 17 ] permethrin induced DNA methylation epimutation markers in sperm of rats, [ 18 ] male reproductive toxicity of deltamethrin, [ 19 ] impairment of Leydig and Sertoli cell function and reproductive toxicity of bifenthrin, [ 20,21 ] and loss of male reproductive function due to cypermethrin [ 22 ] are some of the studies published in the last one and half years.…”

Section: Introductionmentioning

confidence: 99%

Effect of a mixture of pyrethroids at doses similar to human exposure through food in the Indian context

Ravula

Yenugu

2022

J Biochem & Molecular Tox

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Residual amounts of pyrethroids were detected in rice and vegetables of the Indian market. Thus, consumers are exposed to a mixture of pyrethroids on a daily basis through food. Though a large number of studies reported the toxic effects of pyrethroids, there are no reports that used doses equivalent to human consumption. In this study, male Wistar rats were exposed daily to a mixture of pyrethroids for 1-15 months which is equivalent to the amount present in rice and vegetables consumed by an average Indian each day. The oxidant-antioxidant status (lipid peroxidation, nitric oxide; activities of catalase, glutathione peroxidase, glutathione S transferase, and superoxide dismutase) and anatomical changes in the general organs (liver, lung, and kidney) and male reproductive tract tissues (caput, cauda, testis, and prostate) were evaluated. Further, liver and kidney function tests, lipid profile, and complete blood picture were analyzed.Increased oxidative stress, perturbations in the antioxidant enzyme activities, and damage to the anatomical architecture were observed. Disturbances in the liver function and lipid profile were significant. Results of our study demonstrate that exposure to a mixture of pyrethroids at a dose that is equivalent to human consumption can cause systemic and reproductive toxicity, which may ultimately result in the development of lifestyle diseases. This first line of evidence will fuel further studies to determine the impact of food-based pyrethroid exposure on the long-term health of humans and to envisage policies to reduce pesticide content in food products.

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Key proteins of proteome underlying sperm malformation of rats exposed to low fenvalerate doses are highly related to P53

Cited by 5 publications

References 66 publications

The Abscopal Effects of Cranial Irradiation Induce Testicular Damage in Mice

The Abscopal Effects of Cranial Irradiation Induce Testicular Damage in Mice

A mixture of pyrethroids induces reduced fecundity and increased testicular genotoxicity in rats

Effect of a mixture of pyrethroids at doses similar to human exposure through food in the Indian context

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