Keratinocyte-Melanocyte graft technique followed by PUVA therapy for stable vitiligo

Kachhawa, Dilip; Kalla, G

doi:10.4103/0378-6323.45106

Cited by 25 publications

(22 citation statements)

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“…This is reported by Kachhawa, in which he uses epidermal scrapings (till the level of the papillary dermis) to apply directly onto the dermabraded recipient area. [12] However, questions regarding the homogeneity of the repigmentation, possible multiplication factor from donor skin to treatment area and treatment outcome on larger surface areas remain to be investigated.…”

Section: Methods For Data Collectionmentioning

confidence: 99%

A review of non-cultured epidermal cellular grafting in vitiligo

Geel

Goh

Wallaeys

et al. 2011

J Cutan Aesthet Surg

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Section: Methods For Data Collectionmentioning

confidence: 99%

A review of non-cultured epidermal cellular grafting in vitiligo

Geel

Goh

Wallaeys

et al. 2011

J Cutan Aesthet Surg

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“…Vitiligo can be cosmetically disfiguring and it is a stigmatizing condition leading to serious psychological problems in daily life. Stable vitiligo and lesions with depigmented hairs, indicating the depletion of the melanocyte reservoir in the hair follicle, mostly fail to repigment by conservative therapies [5,6]. In these cases, surgical techniques may be indicated but are often time-consuming and lead to undesired effects [7].…”

Section: Introductionmentioning

confidence: 99%

Some modifications in transplantation of autologus non -cultured melanocytes-keratinocytes suspension in treatment of segmental and focal vitiligo (Egyptian Experience in Alexandria University)

Sobhy¹,

Atia²,

El-Ramly³

2013

Our Dermatol Online

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Introduction: Transplantation of Autologous non-cultured melanocytes suspension is a simple yet effective cell-based therapy for vitiligo. Materials and Methods: 20 patients with stable vitiligo were subjected to epidermal cell suspension transplantation using Osslon's method with some new modifications. Result: The repigmentation at 7 of the test sites (35.0%) was excellent. It was good for more than half of the test sites (55.0%). Fair repigmentation was encountered among only 2 (10.0%) of the tested sites. None of the tested sites showed poor repigmentation. On the other hand, none of the control sites showed excellent or even good repigmentation. However, repigmentation was fair for nearly 10% and it was poor for 90% of the control sites. Conclusion: Autologus non cultured basal -enriched epidermal cell suspension transplantation is an effective, simple and safe method for treatment of stable vitiligo.

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“…[15] Recently, Goh et al have simplified the extraction of epidermal cells from donor skin using a simple inexpensive six-well plate, a microfilter, and only three reagents: trypsin, trypsin inhibitor (soybean), and phosphate buffered saline (PBS). [16] DISCUSSION Non-cultured epidermal suspension is indicated mainly for segmental vitiligo and for stable nonsegmental vitiligo that does not respond to medical treatment.…”

Section: Modifications Of the Initial Techniquementioning

confidence: 99%

Non-cultured epidermal suspension in vitiligo: From laboratory to clinic

Gauthier¹,

Benzekri²

2012

Indian J Dermatol Venereol Leprol

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Background: Medical treatments are ineffective in many patients and surgical methods have therefore been developed. Objective: A review of autologous non-cultured melanocyte grafting techniques is proposed to obtain a successful repigmentation of vitiligo macules. Methods: Initially in 1992, we had developed a simplified grafting method which was carried out in the following two steps: production of blisters on the depigmented lesions by freezing with liquid nitrogen and injection in each blister of a non-cultured suspension of epidermal cells. The cellular suspension was obtained from samples of skin of the hair scalp after trypsinization. This very simple technique could be used at the dermatologist's clinic. Since 1998 (Olsson MJ, Juhlin L), quite comparable but improved and more sophisticated techniques have been proposed for the surgical treatment of vitiligo. These techniques require a laboratory set up to perform the melanocyte transplantation. The donor zone was usually taken on the gluteal region. The time of trypsinization was reduced to 60 minutes at 37°C and the centrifuged cellular suspension added with hyaluronic acid (Van Geel) was directly applied on a dermabraded or laser abraded vitiligo lesions. Results: Whatever the technique chosen, repigmentation was evident within 25 to 30 days. Coalescence of the pigmented areas was spontaneously observed or obtained after UVB radiation. It is obvious that the complete repigmentation occurred more rapidly with the recent techniques compared with the initial method, but the efficiency was quite similar. Conclusion: The use of non-cultured epidermal suspension appears to be an effective, safe, and simple method for treating patients with achromic areas lacking melanocytes.

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Keratinocyte-Melanocyte graft technique followed by PUVA therapy for stable vitiligo

Abstract: For smaller vitiligo patches this method gives cosmetically acceptable results. It is easy to perform and does not require specific laboratory setup.

Cited by 25 publications

References 0 publications

A review of non-cultured epidermal cellular grafting in vitiligo

A review of non-cultured epidermal cellular grafting in vitiligo

Some modifications in transplantation of autologus non -cultured melanocytes-keratinocytes suspension in treatment of segmental and focal vitiligo (Egyptian Experience in Alexandria University)

Non-cultured epidermal suspension in vitiligo: From laboratory to clinic

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