Keratinocyte Differentiation Inversely Regulates the Expression of Transforming Growth Factor-β and Involucrin

Ghahary, A.; Tredget, E. E.; Marcoux, Yvonne

doi:10.1097/00004630-200103002-00030

Cited by 11 publications

(16 citation statements)

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“…To further evaluate the mechanism of G‐CSF stimulation, we analyzed the expression of IL‐1α and IL‐1β in undifferentiated as compared to differentiated keratinocytes by RT‐PCR and found no significant difference between the two cell types (data not shown). However, we have previously demonstrated that keratinocyte differentiation‐state has a dramatic impact upon the expression profile of other proteins such as involucrin and TGF‐β1 [Ghahary et al, ]. Furthermore, we also showed that specifically differentiated keratinocytes are capable of releasing numerous wound healing‐related ECM modulating proteins through an exosome externalization mechanism [Chavez‐Muñoz et al, ].…”

Section: Discussionmentioning

confidence: 97%

Keratinocyte-Releasable Factors Stimulate the Expression of Granulocyte Colony-Stimulating Factor in Human Dermal Fibroblasts

Carr

Rezakhanlou

et al. 2016

J. Cell. Biochem.

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Interaction between keratinocytes and fibroblasts plays a critical role in maintaining skin integrity under both normal and pathological conditions. We have previously demonstrated that keratinocyte-releasable factors influence the expression of key extracellular matrix components, such as collagen and matrix metalloproteinases in dermal fibroblasts. In this study, we utilized DNA microarray analysis to examine the effects of keratinocyte-releasable factors on the expression of several cytokines in human dermal fibroblasts. The results revealed significantly higher granulocyte colony-stimulating factor (G-CSF) expression in fibroblasts co-cultured with keratinocytes relative to mono-cultured cells, which was verified by RT-PCR and western blot. G-CSF is an important hematopoietic factor also thought to play a beneficial role in wound healing through stimulating keratinocyte proliferation. To partially characterize the keratinocyte-releasable factors responsible for stimulating G-CSF production, keratinocyte-conditioned medium (KCM) was subjected to thermal treatment and ammonium sulfate precipitation before treating fibroblasts. The results showed that keratinocyte-releasable G-CSF-stimulating factors remain stable at 56°C and upon 50% ammonium sulfate precipitation. Knowing that keratinocytes release IL-1, which stimulates G-CSF expression in various immune cells, several experiments were conducted to ask whether this might also be the case for fibroblasts. The results showed that the addition of recombinant IL-1 markedly increased G-CSF expression in fibroblasts; however, IL-1 receptor antagonist only partially abrogated KCM-stimulated G-CSF expression, indicating the role of additional keratinocyte-releasable factors. These findings underline the importance of cross-talk between keratinocytes and fibroblasts, suggesting that communication between these cells in vivo modulates the production of cytokines required for cutaneous wound healing and maintenance. J. Cell. Biochem. 118: 308-317, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 97%

Keratinocyte-Releasable Factors Stimulate the Expression of Granulocyte Colony-Stimulating Factor in Human Dermal Fibroblasts

Carr

Rezakhanlou

et al. 2016

J. Cell. Biochem.

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“…Interestingly, proliferative keratinocytes in monolayer culture produce more TGF-b1 than stratified differentiated keratinocytes [36]. The hyperproliferative epidermis in hypertrophic scars also exhibits a high level of TGF-b1 expression [6,7].…”

Section: Discussionmentioning

confidence: 99%

Interactions between myofibroblast differentiation and epidermogenesis in constructing human living skin equivalents

Yang

Hashimoto

Tohyama

et al. 2012

Journal of Dermatological Science

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“…1984) and matrix enzymes such as collagenase (Scharffetter et al. 1991) and metalloproteinase (Ghahary et al. 2001).…”

Section: Discussionmentioning

confidence: 99%

Motogenic substrata and chemokinetic growth factors for human skin cells

2005

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Extracellular matrix remodelling and accurate spatio-temporal coordination of growth factor expression are two factors that are believed to regulate mitoses and cell migration in developing and regenerating tissues. The present quantitative videomicroscopical study examined the influence of some of the principal components of extracellular matrix and several growth factors that are known to be expressed in dermal wounds on three important facets of human skin cell behaviour in culture. Keratinocytes, melanocytes and dermal fibroblasts (and myofibroblast controls) exhibited varying degrees of substrate adhesion, division and migration depending on the composition of the culture substrate. Substrates that are recognized components of transitional matrices generally accentuated cell adhesion and proliferation, and were motogenic, when compared with serum-treated control surfaces, whereas components of more stable structures such as basement membrane had less influence. Platelet-derived growth factor (PDGF), epidermal growth factor (EGF) and α fibroblastic growth factor ( α FGF) all promoted cell proliferation and were chemokinetic to dermal fibroblasts, but not keratinocyte growth factor (KGF) or transforming growth factor β (TGF β ). PDGF, EGF and KGF, but not TGF β or α FGF, all enhanced proliferation of dermal keratinocytes.The same growth factors, and in addition KGF, all stimulated motility in keratinocytes, but TGF β and α FGF again had no effect. Developing a better understanding of the interdependency of factors that control crucial cell behaviour may assist those who are interested in the regulation of histogenesis and also inform the development of rational therapeutic strategies for the management of chronic and poorly healed wounds.

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Keratinocyte Differentiation Inversely Regulates the Expression of Transforming Growth Factor-β and Involucrin

Cited by 11 publications

References 0 publications

Keratinocyte-Releasable Factors Stimulate the Expression of Granulocyte Colony-Stimulating Factor in Human Dermal Fibroblasts

Keratinocyte-Releasable Factors Stimulate the Expression of Granulocyte Colony-Stimulating Factor in Human Dermal Fibroblasts

Interactions between myofibroblast differentiation and epidermogenesis in constructing human living skin equivalents

Motogenic substrata and chemokinetic growth factors for human skin cells

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