Kekerabatan Beberapa Aksesi Padi Lokal Tahan Hama Penyakit Berdasarkan Analisis Polimorfisme Marka SSR

Susanto, Untung; Satoto, Satoto

doi:10.21082/jbio.v12n2.2016.p81-90

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…The GBSS enzyme plays a role in catalyzing the formation of amylose. DNA analysis of the waxy gene allele using the RM190 marker showed that the gene allele is polymorphic [30]. Likewise, the Wx marker was closely related to the waxy gene, which controls the expression of amylose traits.…”

Section: Analysis Of Molecular Markers Associated With Amylose Traits...mentioning

confidence: 99%

Validation of molecular markers associated with amylose content of F8 and F9 lines resulting from a crossing of Black Rice and Mentik Wangi

Suprayogi,

Oktaviani,

Riyanto

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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The development of black rice with a fluffier texture at Universitas Jenderal Soedirman, which began in 2004, was carried out by crossing local black rice with the Mentik Wangi variety. At present, six black rice advanced breeding lines are ready for multi-location yield trials. Meanwhile, developing molecular marker-based plant breeding methods can facilitate a more efficient selection process. An important stage in developing molecular markers is marker validation, which aims to test the effectiveness in determining phenotypes in various genotypic and in new populations that differ from others based on the link between the marker and the expected character identified. This research aimed to determine molecular markers that can differentiate the level of amylose content in populations of F8 and F9 lines. The research was carried out on 10 (ten) genotypes consisting of the control variety Logawa, IRPM 112-19- 56, black rice, Mentik Wangi, and six lines. The amplification of the RM190, Wx and SSIIa markers showed polymorphic band. Based on the simple linear regression analysis, in F9, the SSIIa marker had the highest coefficient of determination compared to other markers. Hence, it had the greatest possibility of being a marker for selecting rice based on amylose content.

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Section: Analysis Of Molecular Markers Associated With Amylose Traits...mentioning

confidence: 99%

Validation of molecular markers associated with amylose content of F8 and F9 lines resulting from a crossing of Black Rice and Mentik Wangi

Suprayogi,

Oktaviani,

Riyanto

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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“…For analyzing the genetic diversity of pines, the average PIC value of 0.44 where this results in hal 7 primers is instructive. A high PIC value indicates that the primer can detect a significant number of alleles [11]. The informative value of a primer is determined according to [12], which states that a primary is classified as informative if its PIC is less than 0.3.…”

Section: Genetic Diversity Analysismentioning

confidence: 99%

Analysis genetic diversity of rombeng pines using RAPD markers

Lailasari,

Mukrimin,

Larekeng

2023

IOP Conf. Ser.: Earth Environ. Sci.

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The tourism industry in Indonesia continues to grow from year to year, as evidenced by the expansion of tourism villages. In the context of tourism, the term’resources’ refers to everything that has the potential to be developed to directly or indirectly support tourism. The natural potential of the Rombeng pine forest (Pinus sp.) in Bantaeng Regency, South Sulawesi, has become a tourist attraction for the surrounding community and visitors from outside the city. This pine species has a trunk characteristic that distinguishes it from scattered pine in general. This trait is manifested by grayish skin and a reddish hue that can be exfoliated. Based on this, an examination of the genetic diversity of pine species in the Bantaeng district was conducted. Utilizing Random Amplified Polymorphic DNA (RAPD) markers and Darwin 6. 0 as a research methodology. This study indicates that the diversity value (HE) of Pinus species is 0.46, which is relatively high. The near category of based genetic distance is dominated by genetic kinship, and the closest genetic distance is 0.04 while the farthest is 0.80.

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Morphological Variability in Leaf of Indonesian Rice Landraces and Its Correlation to Bacterial Leaf Blight Disease Resistance

Rohaeni¹,

Yuliani²

2019

JIPI

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Hawar daun bakteri (HDB) adalah salah satu penyakit utama dan penting pada tanaman padi yang disebabkan oleh bakteri patogen Xanthomonas oryzae pv. oryzae (Xoo), terutama padi sawah irigasi. Morfologi daun menjadi karakter yang menarik untuk diinvestigasi hubungannya dengan intensitas penyakit HDB karena daun merupakan bagian tanaman yang diserang oleh penyakit HDB. Tujuan penelitian ini adalah untuk memperoleh informasi keragaman morfologi daun dan korelasinya dengan penyakit HDB untuk mendukung program perakitan varietas unggul tahan HDB. Sebanyak 25 aksesi padi lokal dikarakterisasi secara fenotipik. Pengujian karakter morfologi dilakukan tanpa menggunakan ulangan, sedangkan uji ketahanan terhadap Xoo (patotipe IV) dilakukan dengan menggunakan rancangan percobaan acak kelompok (tiga ulangan). Hasil uji morfologi daun menunjukkan semua aksesi padi lokal memiliki leher berwarna hijau, warna telinga transparan, warna lidah putih, dan bentuk lidah 2-cleft. Pada 4 minggu setelah inokulasi (MSI) terdapat empat aksesi (Ketan Lomah Hitam, Bumbuy Inih, Gonggoi, dan Waren) yang menunjukkan persentase keparahan terhadap penyakit HDB antara 1316%, dan nilai ini mengindikasikan tingkat ketahanan yang agak rentan terhadap penyakit HDB dan lebih baik dari aksesi lainnya. Morfologi permukaan daun berkorelasi positif dengan ketahanan terhadap HDB, dan keempat aksesi tersebut memiliki permukaan daun berbulu atau sedikit berbulu dan daun berukuran pendek. Hasil penelitian ini dapat dijadikan sebagai referensi dalam proses seleksi pada program perakitan varietas unggul baru yang tahan penyakit HDB.

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Kekerabatan Beberapa Aksesi Padi Lokal Tahan Hama Penyakit Berdasarkan Analisis Polimorfisme Marka SSR

Cited by 3 publications

References 19 publications

Validation of molecular markers associated with amylose content of F8 and F9 lines resulting from a crossing of Black Rice and Mentik Wangi

Validation of molecular markers associated with amylose content of F8 and F9 lines resulting from a crossing of Black Rice and Mentik Wangi

Analysis genetic diversity of rombeng pines using RAPD markers

Morphological Variability in Leaf of Indonesian Rice Landraces and Its Correlation to Bacterial Leaf Blight Disease Resistance

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