The chromosomal data (chromosome number and morphology, Ag-NOR and C-banding patterns) of endemic Luciobarbus kottelati was presented from Turkey. The diploid chromosome number was found to be 2n=100. The karyotype consisted of nine pairs of metacentric, 17 pairs of submetacentric and 24 pairs of subteloacrocentric chromosomes (Fundamental number, Fn=152). Evident C-bands were on the pericentromeric regions of three chromosome pairs. The number of Ag-NOR bearing chromosomes varied from two to six. This study is a contribution to barbels chromosomal knowledge.