Karyotypes of Saccharomyces sensu lato species

Petersen, Randi Føns; Nilsson-Tillgren, Torsten; Piškur, Jure

doi:10.1099/00207713-49-4-1925

Cited by 45 publications

(59 citation statements)

References 25 publications

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“…A recent study showed at least 12 bands (570^1950 kb) for an estimated genome size of 12.3 Mb [8]. Similar patterns were obtained with S. unisporus isolates, consistent with the fact that these species are closely related [9].…”

Section: Introductionsupporting

confidence: 79%

Genomic Exploration of the Hemiascomycetous Yeasts: 7. Saccharomyces servazzii

Casarégola¹,

Lépingle²,

Bon³

et al. 2000

FEBS Letters

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The genome of Saccharomyces servazzii was analyzed with 2570 random sequence tags totalling 2.3 Mb. BLASTX comparisons revealed a minimum of 1420 putative open reading frames with significant homology to Saccharomyces cerevisiae (58% aa identity on average), two with Schizosaccharomyces pombe and one with a human protein, confirming that S. servazzii is closely related to S. cerevisiae. About 25% of the S. servazzii genes were identified, assuming that the gene complement is identical in both yeasts. S. servazzii carries very few transposable elements related to Ty elements in S. cerevisiae. Most of the mitochondrial genes were identified in eight contigs altogether spanning 25 kb for a predicted size of 29 kb. A significant match with the Kluyveromyces lactis linear DNA plasmid pGKL-1 encoded RF4 killer protein suggests that a related plasmid exists in S. servazzii. The sequences have been deposited with EMBL under the accession numbers AL402279Â L404848. ß 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

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Section: Introductionsupporting

confidence: 79%

Genomic Exploration of the Hemiascomycetous Yeasts: 7. Saccharomyces servazzii

Casarégola¹,

Lépingle²,

Bon³

et al. 2000

FEBS Letters

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“…Previous studies have shown that the species of Saccharomyces sensu stricto exhibit relatively homogeneous karyotypes compared with those of species of Saccharomyces sensu lato (Vaughan-Martini et al, 1993;Fischer et al, 2000;Naumov et al, 1992Naumov et al, , 1995Petersen et al, 1999;Lu et al, 2004;Wu & Bai, 2005). The electrophoretic karyotypes of strains H-6 T , ZX-15 and ZX-20 bore a superficial resemblance to those of the accepted Saccharomyces species, being consistent with their placement in this genus based on rDNA sequence analysis (Fig.…”

Section: Electrophoretic Karyotypingsupporting

confidence: 65%

Saccharomyces arboricolus sp. nov., a yeast species from tree bark

Wang

Bai

2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Three ascomycetous yeast strains, H-6 T , ZX-15 and ZX-20, isolated from the bark of two tree species of the family Fagaceae collected from different regions of China, formed unconjugated and persistent asci containing two to four globose ascospores. 26S rDNA D1/D2 domain and internal transcribed spacer (ITS) region (including 5.8S rDNA) sequence analysis showed that they were closely related to the currently accepted Saccharomyces species with strong support. Comparisons of the rDNA sequences, electrophoretic karyotypes and physiological characters indicated that the three strains represent a novel species in the genus Saccharomyces. The name Saccharomyces arboricolus sp. nov. was proposed for the novel species, with H-6 T (5AS 2.3317 T 5CBS 10644 T ) isolated from the bark of Quercus fabri as the type strain.Seven species are currently included in the genus Saccharomyces Meyen ex Reess as redefined recently by Kurtzman (2003), based on multigene sequence analysis (Kurtzman & Robnett, 2003). During the investigation of the diversity of ascomycetous yeasts associated with plant materials from China, three strains, H-6 T , ZX-15 and ZX-20, isolated from the bark of broadleaf trees, were found to represent a novel species of the genus Saccharomyces by physiological characterization, rRNA gene sequencing and electrophoretic karyotyping.Yeast strains living in bark were isolated by using the enrichment method. Pieces of bark with or without exudates from independent trees were cut with a sterile scalpel and placed into sterile plastic tubes containing 3 ml liquid enrichment medium. The medium was made according to Sniegowski et al. (2002) with minor modifications, containing (w/v) 0.3 % yeast extract, 0.3 % malt extract, 0.5 % peptone, 0.5 % sucrose, 0.5 % galactose, 7.6 % (v/v) ethanol, 200 mg chloramphenicol ml 21 and 1 ml 1 M HCl per litre. The cultures were incubated at room temperature without shaking for 7 to 14 days. Aliquots (100 ml) of the 10 22 to 10 24 diluted enrichment culture was spread on plate medium made by adding 2 % agar to the liquid enrichment medium. After 2 days incubation at room temperature or 25 u C, colonies with different morphological characters were transferred into malt extract agar slants for further purification and examination.

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“…The strain used in this work (type strain CBS3082) is diploid. Petersen et al [1] estimated that S. kluyveri contains seven chromosomes with a 10 Mb genome size, in agreement with Vaughan-Martini et al [2]. Weinstock and Strathern [3], however, observed eight separate chromosomal bands by pulsed ¢eld gel electrophoresis of strain CBS4568, all larger than 0.9 Mb.…”

Section: Introductionsupporting

confidence: 63%

Genomic Exploration of the Hemiascomycetous Yeasts: 9. Saccharomyces kluyveri

et al. 2000

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The genome of Saccharomyces kluyveri was explored through 2528 random sequence tags with an average length of 981 bp. The complete nuclear ribosomal DNA unit was found to be 8656 bp in length. Sequences homologous to retroelements of the gypsy and copia types were identified as well as numerous solo long terminal repeats. We identified at least 1406 genes homologous to Saccharomyces cerevisiae open reading frames, with on average 58.1% and 72.4% amino acid identity and similarity, respectively. In addition, by comparison with completely sequenced genomes and the SwissProt database, we found 27 novel S. kluyveri genes. Most of these genes belong to pathways or have functions absent from S. cerevisiae, such as the catabolic pathway of purines or pyrimidines, melibiose fermentation, sorbitol utilization, or degradation of pollutants. The sequences are deposited in EMBL under the accession numbers AL404849^AL407376. ß

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Karyotypes of Saccharomyces sensu lato species

Cited by 45 publications

References 25 publications

Genomic Exploration of the Hemiascomycetous Yeasts: 7. Saccharomyces servazzii

Genomic Exploration of the Hemiascomycetous Yeasts: 7. Saccharomyces servazzii

Saccharomyces arboricolus sp. nov., a yeast species from tree bark

Genomic Exploration of the Hemiascomycetous Yeasts: 9. Saccharomyces kluyveri

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