Karyotypes of 3- or 4-day-old pig embryos after short in-vitro culture

Hoeven, F.A. van der; Cuijpers, M. P.; Boer, P. de

doi:10.1530/jrf.0.0750593

Cited by 20 publications

(14 citation statements)

References 16 publications

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“…As for comparison of our findings with previous data obtained in pigs by karyotyping, we can compare the total number of aberrant embryos detected. Our finding of 11.4 % approximates the data published by McFeely (1967) and by Van der Hoeven et al (1985) who detected 9.4 and 7.3 % of aberrant embryos, respectively.…”

Section: Discussionsupporting

confidence: 90%

Aneuploidy detection in porcine embryos using fluorescence in situ hybridization

Zudová

Kubı́čková

et al. 2003

Cytogenet Genome Res

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In contrast to human embryos, there are very few studies published on the frequency of chromosomal aneuploidy in farm animals. The objectives of this study were to apply a three-color fluorescent in situ hybridization (FISH) method for evaluating aneuploidy in porcine embryos using chromosome-specific DNA probes, establish baseline frequencies of aneuploidy in embryos and compare the results with our previous findings of aneuploidy in spermatozoa and oocytes. The embryos were collected from superovulated gilts, which were slaughtered 48 h after insemination. FISH was performed using probes specific for the centromeric regions of porcine chromosomes 1, 10 and Y. Altogether 403 blastomeres from 114 porcine embryos were successfully investigated. Diploidy was observed in 101 (88.6%) embryos, triploidy in 2 (1.8%) embryos, mosaicism/mixoploidy in 9 (7.9%) embryos, and trisomy for chromosomes 1 or 10 in 2 (1.8%) embryos. No blastomere showed aneuploidy for chromosome Y. These findings correspond with the frequencies of aneuploidy we have found previously in porcine germ cells.

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Section: Discussionsupporting

confidence: 90%

Aneuploidy detection in porcine embryos using fluorescence in situ hybridization

Zudová

Kubı́čková

et al. 2003

Cytogenet Genome Res

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“…The embryo is regarded as 0Y. embryos, ploidy anomalies were seen in 7.3% of embryos [Van der Hoeven et al, 1985]; however, in in vitro embryos, the ploidy anomaly rate increased to 45.9% [Ulloa et al, 2008]. To explain this, an increased frequency of aneuploidies in embryos produced in vitro in comparison to those obtained in vivo was evidenced [Slimane-Bureau and King, 2002].…”

Section: Discussionmentioning

confidence: 99%

Investigation of Chromosome Aneuploidies in Early Porcine Embryos Using Comparative Genomic Hybridization

Horňák

Hulinska

Musilová

et al. 2009

Cytogenet Genome Res

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Although numerical chromosome errors are known to be prevalent in early human embryos and are likely to be a considerable factor influencing the mortality of early embryos and implantation failure, in domestic animals data about the frequency and nature of errors is limited. The objectives of this study were to investigate the whole chromosome set of in vivo obtained early pig embryos, applying methods of whole genome amplification and comparative genomic hybridization (CGH) and to contribute to the comprehensive understanding of the topic. The embryos were collected from gilts 72 h after insemination. Further, they were lysed and underwent whole genome amplification by multiple displacement amplification. In a subsequent CGH, amplified DNA was compared to control DNA using different fluorescent labeling and hybridization to male pig mitoses. 11 (14.3%) of the 77 pig embryos examined were observed to be aneuploid. We found chromosome errors comprising loss/gain of one or a few chromosomes (10.4%) but also extensive chromosome imbalances (3.9%). Chromosomes 8, 11, 12, 13, 17, and X were most frequently involved in aneuploidies, when compared to chromosomes 2, 9, and 18, which were rarely involved in chromosome errors.

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“…Embryos produced by IVP have a high incidence of chromosomal abnormalities, including mixoploidy and polyploidy; these have been linked to a high frequency of polyspermic fertilization [12][13][14]. Cytogenetic analysis of porcine blastocysts produced in vitro has revealed that about 40% of the blastocysts are abnormal [15]; this is higher than the 7.3% reported for in vivo-derived embryos [16]. In cattle, the incidence of mixoploidy is about three times higher in embryos produced by IVP than in those produced in vivo [17].…”

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confidence: 91%

The Blastocyst Production Rate and Incidence of Chromosomal Abnormalities by Developmental Stage in In Vitro Produced Porcine Embryos

Ullo

Yoshizawa

Komoriya

et al. 2008

Journal of Reproduction and Development

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Abstract. The present study was conducted to determine the relationship between embryonic development speed at different stages (the cleaved stage at 52 h and the blastocyst stage at 6 days post insemination) and incidences of chromosome abnormalities in in vitro produced porcine embryos. Porcine oocytes were collected from 3-6-mm ovarian follicles obtained at a slaughterhouse and matured in modified NCSU-37 medium for 44-46 h. Following in vitro fertilization with a final concentration of 1 × 10 5 sperm/ml for 3 h, all oocytes were cultured in vitro for 52 h. Day-2 (52 h after insemination) embryos were classified according to their cleaved stages into 2-cell, 3-to 4-cell, 5-to 8-cell, and >8-cell stages; these were cultured separately for additional 4 days (Day 6). The resultant Day-6 blastocysts were classified according to the morphological diameter into 3 grades: Grade A, expanded blastocysts; Grade B, expanding blastocysts; and Grade C, early blastocysts. They were then analyzed chromosomally. The 3-to 4-cell and 5-to 8-cell embryos had significantly high blastocyst development rates (46.1 and 36.9%, respectively), and these blastocysts contained significantly more cells (40.2 and 42.4 cells, respectively) than those derived from 2-cell embryos and >8-cell embryos (28.6 and 26.5 cells, respectively). The incidence of chromosomal abnormalities was significantly higher in the blastocysts derived from 2-cell and >8-cell stage embryos than in the blastocysts derived from the other stage embryos. Furthermore, the grade A blastocysts had the lowest incidence of chromosomal abnormalities (35.3%) and contained the most cells (48.7 cells). Porcine in vitro production (IVP) yielded a high blastocyst rate and an excellent embryo quality when 3-to 4-cell and 5-to 8-cell stage embryos were selected on Day 2 after insemination. The same criteria yielded a higher quality of expanded blastocysts based on the stage of embryo development and morphology. Key words: Blastocyst, Chromosomal abnormality, Embryo, In vitro production (IVP), Pig (J. Reprod. Dev. 54: [22][23][24][25][26][27][28][29] 2008) n mammals, the technology of in vitro production (IVP) of embryos, which involves in vitro maturation and fertilization of oocytes and in vitro culture of the resultant embryos, has become important for increasing the number of offspring from selected high-quality animals and for reducing the generation intervals for breed improvement in livestock [1,2]. Moreover, IVP technology has been used in the production of cloned and transgenic animals, which can be used in biomedical research [3][4][5]. In swine, although embryos have been successfully produced using the IVP system, the development rates to the blastocyst stage and to full term after embryo transfer are still low [6][7][8]. The viability of porcine embryos produced by IVP is low compared with that of in vivo-derived porcine embryos. For example, porcine embryos produced by IVP are of poor quality in terms of the number of cells compared with those produced in vivo [9].A...

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Karyotypes of 3- or 4-day-old pig embryos after short in-vitro culture

Abstract: Summary. Pig embryos (3\p=n-\4days old) were

Cited by 20 publications

References 16 publications

Aneuploidy detection in porcine embryos using fluorescence in situ hybridization

Aneuploidy detection in porcine embryos using fluorescence in situ hybridization

Investigation of Chromosome Aneuploidies in Early Porcine Embryos Using Comparative Genomic Hybridization

The Blastocyst Production Rate and Incidence of Chromosomal Abnormalities by Developmental Stage in In Vitro Produced Porcine Embryos

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