KAJIAN FILOGENETIK IKAN TUNA (Thunnus spp) SEBAGAI DATA PENGELOLAAN DI PERAIRAN SEKITAR KEPULAUAN MALUKU, INDONESIA

Abstract

Tuna (Thunnus Spp) is a pelagic fish that has migration capabilities and commercial value. The condition of North Maluku and Ambon oceanographies supports the abundance of resource population stocks. Phylogenetic knowledge can help show the evolutionary relationship of an organism inferred from morphological and molecular data. The aim of the study was to determine phylogenetic of tuna in the waters of North Maluku and Ambon. This study used the PCR-Sequencing method. Molecular analysis uses a polymerase chain reaction (PCR) with CRK-CRE primer, electrophoresis. Reconstruction of the phylogenetic tree using the Neighbor joining method and the Kimura 2-parameter evolution model was carried out with the MEGA5 application. The results of phylogenetic research found that there were four four different clades of tuna species (bigeye tuna; kuing fin tuna; alalunga tuna; cakalang). The genetic distance of big eye tuna (T.obesus) with yellow fin tuna (T.albacares) is 0.09, bigeye tuna (T.obesus) with tuna alalunga (T.albacore) is 0.19, yellow fin tuna (T.albacares) with tuna alalunga (T.albacore), for 0.21, big eye tuna (T.obesus) with alalunga tuna (T.albacore) cakalang (K.pelamis) is 0.34, cakalang (K.pelamis) with alalunga tuna (T.albacore) is 0.39 and yellow fin tuna (T.albacares) with Cakalang (K. pelamis) are 0.34. All results show significant genetic differences, genetic tuna species come from one group and filogeography has a real distribution boundary between one another.

Phylogenetic analysis of the grouper family (Serranidae) from various local markets in Indonesian waters using COI (cytochrome oxidase I)

“…Science phylogenetics can predict evolution that occurred in the past by comparing sequences DNA or Protein. Phylogenetics can show the evolutionary relationship of an organism from morphological and molecular data (Akbar et al, 2018). Species identification using mitochondrial DNA has the advantage of its size smaller, large number of regions, complete DNA sequence information available for aquatic organisms, and no non-coding ranges.…”