Kadanguslactones A-E, further oxygenated terpenoids from Kadsura angustifolia fermented by a symbiotic endophytic fungus, Penicillium ochrochloron SWUKD4.1850

Qin, Dan; Shen, Weiyun; Gao, Tiancong; Zuo, Shihao; Song, Hongchuan; Xu, Jieru; Yu, Baohong; Peng, Yajun; Guo, Jiali; Tang, Wenwen; Dong, Jinyan

doi:10.1016/j.phytochem.2020.112335

Cited by 13 publications

(15 citation statements)

References 41 publications

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“…This finding demonstrated that triterpenoids play an important regulatory role in endophytic communities of S. sphenanthera. Moreover, the endophytic fungus Penicillium sp., which was significantly promoted by triterpenoids, was able to convert and modify host triterpenoids in our previous findings [28]. Previous studies showed that endophytic fungi from Armoracia rusticana could decompose glucosinolates by utilizing them as a carbon source [52].…”

Section: Discussionmentioning

confidence: 58%

“…Since 1970, more than 1000 terpenetypes of schtriterpenoids/schnortriterpenoids have been reported to be produced by the plants of Schisandraceae family [27]. Our previous studies demonstrated that endophytic fungi can specifically utilize and transform host triterpenoids in vitro [28,29]. Thus, triterpenoids may play a role in shaping the endophytic microbiome in the plant.…”

Section: Introductionmentioning

confidence: 99%

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Plant Triterpenoids Regulate Endophyte Community to Promote Medicinal Plant Schisandra sphenanthera Growth and Metabolites Accumulation

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Qin

Wang

et al. 2021

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Beneficial interactions between endophytes and plants are critical for plant growth and metabolite accumulation. Nevertheless, the secondary metabolites controlling the feedback between the host plant and the endophytic microbial community remain elusive in medicinal plants. In this report, we demonstrate that plant-derived triterpenoids predominantly promote the growth of endophytic bacteria and fungi, which in turn promote host plant growth and secondary metabolite productions. From culturable bacterial and fungal microbial strains isolated from the medicinal plant Schisandra sphenanthera, through triterpenoid-mediated screens, we constructed six synthetic communities (SynComs). By using a binary interaction method in plates, we revealed that triterpenoid-promoted bacterial and fungal strains (TPB and TPF) played more positive roles in the microbial community. The functional screening of representative strains suggested that TPB and TPF provide more beneficial abilities to the host. Moreover, pot experiments in a sterilized system further demonstrated that TPB and TPF play important roles in host growth and metabolite accumulation. In summary, these experiments revealed a role of triterpenoids in endophytic microbiome assembly and indicated a strategy for constructing SynComs on the basis of the screening of secondary metabolites, in which bacteria and fungi join forces to promote plant health. These findings may open new avenues towards the breeding of high yielding and high metabolite-accumulating medicinal plants by exploiting their interaction with beneficial endophytes.

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Section: Discussionmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Plant Triterpenoids Regulate Endophyte Community to Promote Medicinal Plant Schisandra sphenanthera Growth and Metabolites Accumulation

You

Qin

Wang

et al. 2021

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“…A. C. Smith, which were collected in November (dry season) 2012 at Xichou (N23°26′ 18.10″, E104°40′19.92″) in the Yunnan province of China [17]. The isolate SWUKD4.1850 was taxonomically identified as P. ochrochloron by the authors according to the morphology and sequence analysis of the ITS region of the rDNA, which has been described in detail in our previous study [15,16]. A stock culture of P. ochrochloron SWUKD4.1850 was deposited in the Key Laboratory of Eco-environments at Three Gorges Reservoir Region of Ministry of Education, School of Life Science, Southwest University, Chongqing, China.…”

Section: Fungal Materialsmentioning

confidence: 98%

“…Compounds with antimicrobial activity, namely, (−) 2,3,4-trihydroxybutanamide and (−) erythritol, have been also reported [14]. In our previous studies, the endophytic fungus P. ochrochloron SWUKD4.1850, isolated from Kadsura angustifolia, was able to transform nigranoic acid, a bioactive triterpene from K. angustifolia, into highly oxygenated terpenoids when cocultured with host plant materials [15][16][17]. In order to search for further bioactive substances from endophytic fungi, the mycelial solid culture of P. ochrochloron SWUKD4.1850 was selected for chemical investigation.…”

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confidence: 99%

Resorcylic Acid Lactones Produced by an Endophytic Penicillium ochrochloron Strain from Kadsura angustifolia

et al. 2020

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Four new β-resorcylic acid lactones, including penochrochlactone A (2), 4-O-desmethyl-aigialomycin B (4), and penochrochlactones C and D (5 and 6), two compounds isolated from a natural source for the first time, 5α, 6β-acetonide-aigialomycin B (1) and penochrochlactone B (3), together with six known compounds, aigialomycin F (7), aigialomycins A, B, and D (8–10), zeaenol (11), and oxozeaenol (12), were isolated from a mycelial solid culture of the endophytic fungus Penicillium ochrochloron SWUKD4.1850 from the medicinal plant Kadsura angustifolia by sequential purification over silica gel, Sephadex LH-20 column chromatography, and preparative HPLC. Their structures were elucidated by extensive spectroscopic analysis and chemical conversions. In addition, all the new compounds were evaluated for their cytotoxic and antibacterial activities in vitro. Penochrochlactone C (5) displayed moderate cytotoxicity against the HeLa tumor cell line with an IC50 value of 9.70 µM. In the antibacterial assays, compounds 4 – 6 exhibited moderate activities against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa with MIC values between 9.7 and 32.0 µg/mL.

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“…A wide variety of novel metabolites produced by endophytes have exhibited diverse biological activities (Pimentel et al 2011). The endophytic microbiome can serve as a reservoir of important secondary metabolites, including antibiotics, anticancer molecules, and antioxidants (Qin et al 2020). Many reports suggest that bacterial endophytes have great potential as plant growth promoters (Barac et al 2004;Chauhan et al 2013;Balla et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Bacterial Endophytes from Ramie (Boehmeria nivea)

2021

IJAB

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Endophytic bacteria provide several advantages to the host plant by promoting growth as well as conferring resistance against pathogens and toxic compounds. More than 90% of the cultivation of ramie [Boehmeria nivea (L.) Gaud] is contributed by China. In our study, 265 isolates of bacterial endophytes were obtained from ramie plants. The isolates were then subjected to PCR-based screening. Four endophytic bacterial phyla were identified using 16S rDNA sequencing. The identified dominant microorganisms were Janibacter melonis, Moraxella, and Bacillus pumilus in the root, stem, and leaf, respectively. The results suggest that these endophytes isolated from the ramie have the potential to act as biocontrol agents against pathogens such as Sclerotinia, Colletotrichum, and Phytophthora. However, further study is required to examine the potential application of bacterial endophytes, particularly as biological control agents. © 2021 Friends Science Publishers

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Kadanguslactones A-E, further oxygenated terpenoids from Kadsura angustifolia fermented by a symbiotic endophytic fungus, Penicillium ochrochloron SWUKD4.1850

Cited by 13 publications

References 41 publications

Plant Triterpenoids Regulate Endophyte Community to Promote Medicinal Plant Schisandra sphenanthera Growth and Metabolites Accumulation

Plant Triterpenoids Regulate Endophyte Community to Promote Medicinal Plant Schisandra sphenanthera Growth and Metabolites Accumulation

Resorcylic Acid Lactones Produced by an Endophytic Penicillium ochrochloron Strain from Kadsura angustifolia

Isolation and Characterization of Bacterial Endophytes from Ramie (Boehmeria nivea)

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