K+ and Na+ absorption by outer sulcus epithelial cells

Marcus, Daniel C.; Chiba, Tsuneo

doi:10.1016/s0378-5955(99)00074-x

Cited by 57 publications

(37 citation statements)

References 21 publications

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“…The activation of P2X 2 leads to open ATP-sensitive nonselective cation channels (Lee et al, 2001), a process thought to protect the ear from overstimulation (above). The ATP-insensitive channel helps maintain the low Na + and Ca 2+ levels of endolymph (Marcus and Chiba, 1999). The basolateral K + conductance establishes the driving force for cation absorption ).…”

Section: Apical (Endolymphatic Side) Receptorsmentioning

confidence: 99%

Purinergic signaling in the inner ear

Lee

Marcus

2008

Hearing Research

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Epithelial cells of the inner ear coordinate their ion transport activity through a number of mechanisms. One important mechanism is the autocrine and paracrine signaling among neighboring cells in the ear via nucleotides, such as adenosine, ATP and UTP. This review summarizes observations on the release, detection and degradation of nucleotides by epithelial cells of the inner ear. Purinergic signaling is thought to be important for endolymph ion homeostasis and for protection from over stimulation.

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Section: Apical (Endolymphatic Side) Receptorsmentioning

confidence: 99%

Purinergic signaling in the inner ear

Lee

Marcus

2008

Hearing Research

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“…The study was approved by the Animal Care and Use Committee at the West China Medical School of Sichuan University. The temporal bone was removed and StV and NSt tissues were dissected at 4ºC, as previously described [17].…”

Section: Animalsmentioning

confidence: 99%

The expression of endothelin type A and B receptors in the lateral wall of the mouse cochlea

Luo

Tang

Xia

et al. 2007

Cellular and Molecular Biology Letters

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Endothelin (ET), originally characterized as a vasoconstrictive peptide, has been found to have many different biological functions, including acting as a local hormonal regulator of pressure, fluid, ions and neurotransmitters in the inner ear. The objective of this study was to examine and quantify the mRNA expression of the endothelin type A and B receptors (ETAR and ETBR) in the strial vascularies (StV) and non-strial tissues (NSt) of the cochlear lateral wall using the real-time quantitative reverse transcriptionpolymerase chain reaction (RT-PCR) technique. The mouse tissue samples were harvested and RNA was extracted. RT was performed to obtain cDNA, and then the mRNA expression of each gene was measured via real-time PCR. We found that both receptor subtypes were expressed in the cochlear lateral wall, with a predominance of ETAR over ETBR. We showed that the mRNA expression of the two receptor subtypes was higher in the StV with a 1.8 times higher level of ETAR and an 8.1 times higher level of ETBR mRNAs than in the adjacent NSt of the lateral wall tissue. This study shows the existence and the quantity of ET receptor subtypes in the StV and NSt of the mouse cochlea. Our results suggest that an endothelin-mediated response via two different receptors, ETAR and ETBR, may play an important role in the physiological functions of the cochlear lateral wall by maintaining the homeostatic environment of the cochlea. Unauthenticated

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“…Epithelial cells of the Reissner's membrane (RM) (Lee and Marcus, 2003;C. H. Kim et al, 2009b; S. H. Kim et al, 2009) and outer sulcus cells (Marcus and Chiba, 1999;Lee et al, 2001) were reported to contribute to endolymphatic homeostasis by absorbing Na ϩ . The RM consists of two cell layers (tight epithelia, which face endolymph, and mesothelia, which face perilymph) that are separated by a basement membrane and a thin layer of intercellular substance.…”

Section: Introductionmentioning

confidence: 99%

P2Y₄-Mediated Regulation of Na⁺Absorption in the Reissner's Membrane of the Cochlea

Kim¹,

Kim²,

Lee³

et al. 2010

J. Neurosci.

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The epithelial cells of Reissner's membrane (RM) are capable of transporting Na ϩ out of endolymph via epithelial Na ϩ channel (ENaC). However, much remains to be known as to mechanism of regulation of Na ϩ absorption in RM. We investigated P2Y signaling as a possible regulatory mechanism of ENaC in gerbil RM using voltage-sensitive vibrating probe technique and immunohistochemistry. Results showed that UTP induced partial inhibition of the amiloride-sensitive short-circuit current but did not change short-circuit current when applied in the presence of amiloride. The inhibitory effect of UTP was not completely reversible in minutes. The response to UTP was inhibited by reactive blue-2 and 2Ј,3Ј-O-(4-benzoylbenzoyl)adenosine 5Ј-triphosphate but not by suramin or pyridoxalphosphate-6-azophenyl-2Ј, 4Ј-disulfonic acid, which indicates this P2Y receptor as the P2Y 4 subtype. The phospholipase C (PLC) inhibitors 1-[6[[(17␤)-3-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1H-pyrrole-2,5-dione and 1-O-octadecyl-2-O-methyl-rac-glycero-3-

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K+ and Na+ absorption by outer sulcus epithelial cells

Cited by 57 publications

References 21 publications

Purinergic signaling in the inner ear

Purinergic signaling in the inner ear

The expression of endothelin type A and B receptors in the lateral wall of the mouse cochlea

P2Y₄-Mediated Regulation of Na⁺Absorption in the Reissner's Membrane of the Cochlea

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K+ and Na+ absorption by outer sulcus epithelial cells

Cited by 57 publications

References 21 publications

Purinergic signaling in the inner ear

Purinergic signaling in the inner ear

The expression of endothelin type A and B receptors in the lateral wall of the mouse cochlea

P2Y4-Mediated Regulation of Na+Absorption in the Reissner's Membrane of the Cochlea

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P2Y₄-Mediated Regulation of Na⁺Absorption in the Reissner's Membrane of the Cochlea