Joint reconstruction of an in-focus image and of the background signal in in-line holographic microscopy

Berdeu, Anthony; Thomas, O.; Momey, Fabien; Denis, Loïc; Pinston, Frédéric; Faure, Nicolas; Fournier, Corinne

doi:10.1016/j.optlaseng.2021.106691

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2022

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“…Digital in-line holography (DIH) using a spherical wave as the illuminating source, diffracted via a pinhole, is a facile realization strategy of holographic microscopy [1,2]. The object is placed behind the pinhole with a short distance, and the scattered beam of the object forms the interference fringes with the un-scattered reference beam, considered as an ideal spherical wave.…”

Section: Introductionmentioning

confidence: 99%

Two-Step Converging Spherical Wave Diffracted at a Circular Aperture of Digital In-Line Holography

Tian

Guo

et al. 2022

Micromachines

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The aspheric light emitted from a pinhole restrains the reconstruction quality of a digital in-line hologram. Herein, the Fresnel-diffracted spot from the first step converging spherical wave diffracted at a rough circular aperture is collimated and expanded to generate an even plane wave, which is converged again by an objective lens and matching a minimum aperture while the central spot is varying from light to dark. We observed that the collected background hologram is filled with a round spot with high contrast as an ideal spherical wave. The resolution board and biology experimental results demonstrated a distinctively reconstructed image without any image processing in a single exposure. The adjustable field of view and magnification, single exposure, and noncontact make it suitable for an online microscope.

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Section: Introductionmentioning

confidence: 99%