Isothermal exponential amplification techniques: From basic principles to applications in electrochemical biosensors

Qi, Hongjie; Yue, Shuzhen; Bi, Sai; Ding, Caifeng; Song, Weiling

doi:10.1016/j.bios.2018.03.065

Cited by 100 publications

(50 citation statements)

References 87 publications

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“…In recent years, owing to the advantages of simplicity and versatility, DNA amplification technology has emerged as an attractive tool in biology field and plays an important role in biomedical research [69][70][71][72][73]. The nucleic acid amplification techniques can be divided into two categories: thermal cycling amplification strategies, like polymerase chain reaction (PCR) [74][75][76], and isothermal amplification strategies, such as rolling circle amplification (RCA) and hybridization chain reaction (HCR) [77][78][79][80][81]. The characteristics of typical DNA amplification technologies as well as the representative works are comprehensively introduced in this section.…”

Section: Dna Amplification Technologymentioning

confidence: 99%

DNA Nanotechnology for Multimodal Synergistic Theranostics

Qiao

Song

et al. 2021

J. Anal. Test.

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Over the past decade, DNA nanotechnology has developed rapidly due to its unique characteristics, such as excellent biocompatibility, high programmability, good predictability, automatically chemical synthesis, and so on. So far, a variety of DNA-based nanostructures, from small to large and simple to complex, have been designed and synthesized with controllable size and shape in one, two, or three dimensions. Therefore, DNA has become a kind of competitive materials for biosensing, bioimaging and biomedicine. In particular, the integration of DNA nanotechnology with multimodal synergistic theranostics can not only achieve accurate cancer diagnosis by the sensitive and accurate detection of cancer biomarkers, but also achieve enhanced anti-cancer therapeutic efficacy, which promote the development of DNA nanotechnology and nanomedicine. In this review, we first give a comprehensive introduction of DNA nanotechnology, and then summarize the DNA self-assembly and amplification strategies for the construction of functional nanoplatforms for multimodal synergistic theranostics. Finally, the challenges and opportunities faced by DNA nanotechnology in biomedicine are discussed.

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Section: Dna Amplification Technologymentioning

confidence: 99%

DNA Nanotechnology for Multimodal Synergistic Theranostics

Qiao

Song

et al. 2021

J. Anal. Test.

Self Cite

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“…Detection of this damage and exploring DNA repair systems are important; hence, more attention is paid to the innovation and development of highly precise techniques. On the other hand, classic methodologies such as PCR cannot recognize type of detected DNA damage, other techniques give chance to detect single cells with DNA damage in a given population (comet assay), analyze the ratio of apoptotic to necrotic cells (FCM and FISH) or detect oxidative damage, and quantify thymine dimers (HPLC-electrospray tandem mass spectrometry and GC-MS) [5,[87][88][89]. Methodology is crucial for sensitive detection of genome DNA damage, characterization of damage type, and quantification of DNA damage and repair mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Review: immunoassays in DNA damage and instability detection

Boguszewska

Szewczuk

Urbaniak

et al. 2019

Cell. Mol. Life Sci.

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The review includes information on the current state of knowledge of immunometric methods with emphasis on the possibility of deoxyribonucleic acid (DNA) damage detection. Beginning with basic immunoassay enzyme-linked immunosorbent assay (ELISA), this review describes methods such as tyramide signal amplification (TSA), enhanced polymer one-step staining (EPOS), and time resolved amplified cryptate emission (TRACE) as improvements of ELISA's developed over time to obtain more accurate results. In the second part of the review, surface plasmon resonance (SPR) and quantum dots (QDs) are presented as the newest outlooks in the context of immunoanalysis of biological material and molecular studies. The aim of this review is to briefly present immunoassays with emphasis on DNA damage detection; therefore, the types of methods are listed and described, types of signal indicators, basic definitions such as antigen and antibody are given. Every method is considered with an exemplary application focusing on DNA studies, DNA damage and instability detection.

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“…Nevertheless, the need for temperature cycles and precise control thereof for proper PCR operation requires sophisticated instrumentation, making its integration with biosensing devices difficult. More recently, nucleic acid amplification techniques that run at a constant temperature have been reported to facilitate the design and development of biosensing platforms with the required sensitivity [29]. Among the different isothermal amplification techniques described, those that work at cellular temperatures (≈37 ºC) are particularly convenient, since they require less energy, which translates into simpler instrumentation and therefore adequate for use at the point of need.…”

Section: Quantified This Technique Is Referred To As Reverse Transcrmentioning

confidence: 99%

“…One of the most prominent aspects of RPA technology is its high reaction speed, even superior to PCR. In this regard, given the absence of thermal cycles, amplification kinetics is conditioned by the activity of the enzymes that work in concert, and RPA has been reported to be faster than other isothermal exponential variants such as Loop-mediated isothermal AMPlification (LAMP) or Helicase-Dependent Amplification (HDA) that occur at 60-65ºC [29]. Here again, RNA targets should be previously retro-transcribed into DNA by a reverse transcriptase and the generated 14 DNA strand, complementary to the RNA target, is subsequently amplified by RPA.…”

Section: Quantified This Technique Is Referred To As Reverse Transcrmentioning

confidence: 99%

Long noncoding RNAs: from genomic junk to rising stars in the early detection of cancer

Miranda‐Castro

de‐los‐Santos‐Álvarez

Lobo-Castañón

2019

Anal Bioanal Chem

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Despite having been underappreciated in favor of their protein-coding counterparts for a long time, long noncoding RNAs (lncRNAs) have emerged as functional molecules, which defy the central dogma of molecular biology, with clear implications in cancer. Altered expression levels of some of these large transcripts in human body fluids have been related to different cancer conditions that turns them into potential non-invasive cancer biomarkers. In this review, a brief discussion about the importance and current challenges in the determination of lncRNAs associated to cancer is provided. Different electrochemical nucleic acid-based strategies for lncRNAs detection are critically described. Future perspectives and remaining challenges for the practical implementation of these methodologies in clinical medicine are also discussed.

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Isothermal exponential amplification techniques: From basic principles to applications in electrochemical biosensors

Cited by 100 publications

References 87 publications

DNA Nanotechnology for Multimodal Synergistic Theranostics

DNA Nanotechnology for Multimodal Synergistic Theranostics

Review: immunoassays in DNA damage and instability detection

Long noncoding RNAs: from genomic junk to rising stars in the early detection of cancer

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