Isoliquiritigenin ameliorates advanced glycation end‐products toxicity on renal proximal tubular epithelial cells

Lin, Chin‐Yi; Lin, Yu‐Cheng; Paul, Catherine Reena; Hsieh, Dennis Jine‐Yuan; Day, Cecilia Hsuan; Chen, Ray‐Jade; Kuo, Chia‐Hua; Ho, Tsung‐Jung; Shibu, Marthandam Asokan; Lai, Chin-Hu; Shih, Tien‐Tsorng; Kuo, Wei‐Wen; Huang, Chih‐Yang

doi:10.1002/tox.23553

Cited by 8 publications

(5 citation statements)

References 18 publications

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“…Afterwards, increased cholesterol synthesis mediated by 3-hydroxy-3-methylglutaryl-coA reductase, cholesterol intake mediated by LDL receptors, and cholesterol outflow mediated by decreased ATP-binding box transporter A1 ultimately lead to lipid accumulation in HK-2 ( 31 ). AGE associated with hyperglycemia was shown to increase tubular epithelial–myofibroblast trans-differentiation (TEMT) and extracellular matrix synthesis, leading to renal fibrosis ( 38 ). It was also proposed that autophagy protects renal TECs from injury in diabetic nephropathy.…”

Section: Discussionmentioning

confidence: 99%

Effect of transferrin glycation induced by high glucose on HK-2 cells in vitro

Zhou

Zhao

et al. 2023

Front. Endocrinol.

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Background and objectiveGlycation is a common post-transcriptional modification of proteins. Previous studies have shown that advanced glycation end modified transferrin (AGE-Tf) levels in diabetic rat kidney tissues were increased; however, its role in diabetic nephropathy remains unclear. In this study, differences in glycation degree and Tf sites induced by differing high glucose concentrations in vitro and the effect on total iron binding capacity (TIBC) were observed. Moreover, the effect of AGE-Tf on human renal tubular epithelial cells (HK-2) was investigated.MethodsIn vitro Tf was incubated with increasing glucose concentrations (0 mM, 5.6 mM, 11.1 mM, 33.3 mM, 100 mM, 500 mM, and 1,000 mM) for AGE-Tf. Differences in AGE-Tf glycation degree and TIBC level were analyzed via colorimetric method. The AGE-Tf glycation sites were identified with LC-MS/MS. HK-2 cells were treated with AGE-Tf prepared with different glucose concentrations (33.3 mM and 500 mM) in vitro. The effects of AGE-Tf on HK-2 cell viability, proliferation, oxidative stress index, and Tf receptor expression levels were then observed.ResultsWith increasing glucose concentrations (100 mM, 500 mM, and 1,000 mM) in vitro, Tf glycation degree was significantly increased. The TIBC levels of AGE-Tf were decreased significantly with increasing glucose concentrations (33.3 mM, 100 mM, 500 mM, and 1,000 mM). Four glycated modification sites in Tf and 17 glycated modification sites were detected in AGE-Tf (500 mM) by LC-MS/MS. The structural types of AGEs were CML, G-H1, FL-1H2O, FL, and MG-H1. No significant differences were found in the survival rate of HK-2 cells among the AGE-Tf (500 mM), AGE-Tf (33.3 mM), and Tf groups (all p > 0.05). The apoptosis rate of HK-2 cells in the AGE-Tf (500 mM) group was significantly higher than that in the AGE-Tf (33.3 mM) group. Additionally, both of them were significantly higher than that in the Tf group (both p < 0.05). The MDA levels of HK-2 cells in the AGE-Tf (500 mM) and AGE-Tf (33.3 mM) groups were higher than that in the Tf group, but not significantly (both p > 0.05). The T-AOC level of HK-2 in the AGE-Tf (500 mM) group was significantly lower than that in the AGE-Tf (33.3 mM) and Tf groups (both p < 0.001). The GSH level of HK-2 cells in the AGE-Tf (500 mM) group was significantly lower than that in the Tf group (p < 0.05). The expression level of TfR in the AGE-Tf (500 mM) group was also significantly lower than that in the Tf group (p < 0.05).ConclusionThe degree and sites of Tf glycation were increased in vitro secondary to high-glucose exposure; however, the binding ability of Tf to iron decreased gradually. After HK-2 was stimulated by AGE-Tf in vitro, the apoptosis of cells was increased, antioxidant capacity was decreased, and TfR expression levels were downregulated.

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Section: Discussionmentioning

confidence: 99%

Effect of transferrin glycation induced by high glucose on HK-2 cells in vitro

Zhou

Zhao

et al. 2023

Front. Endocrinol.

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“…Stachydrine has demonstrated various bioactivities for the treatment of fibrosis, cardiovascular diseases, cancers, uterine diseases, brain injuries, and inflammation [37]. Isoliquiritigenin may prevent diabetic complications by inhibiting rat lens aldose reductase [38]. Liquiritin acts as an antioxidant and has neuroprotective, anticancer, and anti-inflammatory activities [39].…”

Section: Discussionmentioning

confidence: 99%

Effects of Varying Ratios of Glycyrrhiza uralensis and Donkey Hide Gelatin Water Extracts on Dinitrochlorobenzene-Induced Atopic Dermatitis in NC/Nga Mice

Dong

Lee

et al. 2023

Nutrients

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Atopic dermatitis is a chronic skin disease that affects millions of people all over the world. The objective of this study was to evaluate the inhibitory effects of the roots of Glycyrrhiza uralensis (GU) and Donkey Hide Gelatin (DHG) water extracts on DNCB-induced NC/Nga mice and TNF-α/IFN-γ treated keratinocytes or LPS-stimulated macrophages. The combined treatment using the water extracts of GU and DHG improved the skin symptom evaluation score and skin histology, with increased expression of the skin barrier proteins Claudin 1 and Sirt 1 in lesion areas. The IFN-γ activity was promoted in PBMCs, ALN, and dorsal skin tissue, while the absolute cell number was reduced for T cells so that the production and expression of serum IgE and cytokines were suppressed. In TNF-α/IFN-γ induced HaCaT cells, IL-6, IL-8, MDC, and RANTES were all inhibited by GU and DHG water extracts, while ICAM-1 and COX-2 levels were similarly downregulated. In addition, GU and DHG water extracts decreased LPS-mediated nitric oxide, IL-6, TNF-α, and PGE2 in RAW 264.7 cells, and the expression of iNOS and COX-2 also decreased. Notably, the DHG:GU ratio of 4:1 was shown to have the best effects of all ratios. In conclusion, GU and DHG have anti-skin inflammatory potentials that can be used as alternative ingredients in the formula of functional foods for people with atopic dermatitis.

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“…The western blot analysis was performed following previous reports 19,20 . Proteins were separated on 8%–10% Sodium dodecyl sulfate‐polyacrylamide gel and were transferred onto polyvinylidine difluoride (PVDF) membranes (Merck Millipore, MA).…”

Section: Methodsmentioning

confidence: 99%

“…The western blot analysis was performed following previous reports. 19,20 Proteins were separated on 8%-10% Sodium dodecyl sulfate-polyacrylamide gel and were transferred onto polyvinylidine difluoride (PVDF) membranes (Merck Millipore, MA). After incubating in blocking buffer (5% milk, 20 mM Tris-HCl (pH 7.6), 150 mM NaCl, and 0.1% Tween-20) for 1 h, the proteins were hybridized overnight with specific antibodies at 4 C. The membranes were rinsed with Trisbuffered saline with 0.1% Tween ® 20 detergent (TBST) and incubated with the secondary antibody for 1 h. Blots were then visualized with enhanced Chemiluminescence solution and for repeated hybridization the PVDF membranes were stripped by incubating in western blot Stripping buffer for 10 min.…”

Section: Western Blot Analysismentioning

confidence: 99%

Principle active metabolites of Pinus morrisonicolaHayata synergistically inhibit cell proliferation and autophagy to elevate apoptosis in hepatocellular carcinoma cells

Shibu,

Chen,

Yang

et al. 2023

Environmental Toxicology

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Hepatocellular carcinoma (HCC), a common primary tumor of liver is a leading cause of cancer‐associated deaths. Improving cellular apoptosis and enhancing autophagic clearance is been considered to improve treatment outcomes of HCC. Polyphenols from Pinus morrisonicola (Hayata) have shown various physiological and therapeutic benefits and the flavonoid chrysin is been known for their anticancer effects. However, the main bioactive principle and the mechanism underlying the antitumor activity of pine needle extract are not clear yet. In this study, the effects of ethanol extract from pine needle on HCC cells were determined. The results show that when compared with administration of chrysin alone, a fraction containing pinocembrin, chrysin, and tiliroside significantly reduced autophagy and increased apoptosis. The results also correlated with decrease in cell cycle regulators and the autophagic proteins like LC3‐II. Collectively, the results imply the fraction containing pinocembrin, chrysin, and tiliroside as an ideal complementary medicine for an effective antitumor activity.

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Isoliquiritigenin ameliorates advanced glycation end‐products toxicity on renal proximal tubular epithelial cells

Cited by 8 publications

References 18 publications

Effect of transferrin glycation induced by high glucose on HK-2 cells in vitro

Effect of transferrin glycation induced by high glucose on HK-2 cells in vitro

Effects of Varying Ratios of Glycyrrhiza uralensis and Donkey Hide Gelatin Water Extracts on Dinitrochlorobenzene-Induced Atopic Dermatitis in NC/Nga Mice

Principle active metabolites of Pinus morrisonicolaHayata synergistically inhibit cell proliferation and autophagy to elevate apoptosis in hepatocellular carcinoma cells

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