2016
DOI: 10.1016/j.toxicon.2016.02.021
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Abstract: Snake venom is a complex mixture of active compounds consisting of 80-90% proteins and peptides that exhibit a variety of biological actions that are not completely clarified or identified. Of these, phospholipase A2 is one of the molecules that has shown great biotechnological potential. The objectives of this study were to isolate, biochemically and biologically characterize a Lys49 phospholipase A2 homologue from the venom of Bothrops neuwiedi urutu. The protein was purified after two chromatographic steps,… Show more

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Cited by 35 publications
(24 citation statements)
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“…It has been demonstrated that the dimeric form of the protein is essential for the initiation of the Ca 2+ -independent membrane damaging activity [ 33 , 149 ]. As described before, despite being catalyticaly inactive, the sPLA 2 s-like proteins are able to induce marked local myotoxicity and other biological activities, including hyperalgesia and edema [ 23 ].…”
Section: Lys49-pla 2 S a Pla 2 mentioning
confidence: 99%
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“…It has been demonstrated that the dimeric form of the protein is essential for the initiation of the Ca 2+ -independent membrane damaging activity [ 33 , 149 ]. As described before, despite being catalyticaly inactive, the sPLA 2 s-like proteins are able to induce marked local myotoxicity and other biological activities, including hyperalgesia and edema [ 23 ].…”
Section: Lys49-pla 2 S a Pla 2 mentioning
confidence: 99%
“…The figures were generated using the crystal structures of group IA PLA 2 from Naja naja venom (PDB ID 1PSH) and of group III PLA 2 from Apis mellifera venom (PDB ID 1POC). Modified from Fernandes et al, 2014: Biochimica et Biophysica Acta (BBA)-Preoteins and Proteomics, volume 1844, pages 2265-2276, Elsevier, copyright 2014 [ 23 ].…”
Section: Figurementioning
confidence: 99%
“…The PLA 2 activity was characterized by method of Cesari and Boquet (1935), modified by Laraba‐Djebari and Martin‐Eauclaire . Samples (venom, Cc 1 ‐PLA 2 or Cc 2 ‐PLA 2 ), (0.05 absorbance units) are mixed with lecithin 1% (vol/vol) in 0.13 M phosphate buffer, pH 7.4.…”
Section: Methodsmentioning
confidence: 81%
“…The theoretical molecular weight and isoelectric point of Cc 1 ‐PLA 2 and Cc 2 ‐PLA 2 were calculated using a protein analysis tool (Compute pI/Mw tool), available on the Expasy server, and were determined following the methodology used and described by Corrêa et al Both theoretical parameters were compared with those measured previously by Laraba‐Djebari and Martin‐Eauclaire, and Laraba‐Djebari and Chérifi …”
Section: Methodsmentioning
confidence: 99%
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