Isolation of Staphylococcus hominis from Cultured Gilthead Sea Bream (Sparus aurata L.) in Antalya Bay, Turkey

Abstract

In this study, a disease case caused with 15% mortality on gilthead sea bream (Sparus aurata) which was cultured in the Antalya Bay in the Mediterranean coast of Turkey was investigated in July 2015. Lethargy, loss of equilibrium, petechae on the operculums and the dorsal part of the body, pillar of the gills were recorded in the diseased fish. Internally, ascites, splenomegaly and hemorrhages on the intestinal tissue and muscle were observed. Occurrence of parasite was not detected. According to the results of phenotypical test and 16S rDNA sequencing analysis, the isolated bacterial species was identified as Staphylococcus hominis. Histopathologically, hyperplasia of primary lamellae, lamellar telangiectasia, edema and hemorrhages on the gill arch, numerous melanomacrophage centers (MMCs) in the spleen tissue, vacuoler degeneration, necrose and hemorrhages in the liver, multifocal necrosis, and numerous MMC in the kidney were determined and hemorrhages in the tunica propria region of the intestine were observed. It was determined that he isolates showed different susceptibility against antimicrobial agents.

An initial survey on the occurrence of staphylococcal infections in Turkish marine aquaculture (2013–1014)

“…Similar histopathological changes previously reported in staphylococcal infections of fishes (Gaafar et al, 2015;Huang et al, 1999;Timur & Akayli, 2003;Yılmaz et al, 2019) were also observed in the naturally infected fish samples. However, granulomas reported by Huang et al (1999) A two-step pathogenicity assay was designed to confirm the mortalities observed in the field samples and to determine the development stages of staphylococcal infections in the fish tissues.…”

“…Similar histopathological changes previously reported in staphylococcal infections of fishes (Gaafar et al., 2015; Huang et al., 1999; Timur & Akayli, 2003; Yılmaz et al., 2019) were also observed in the naturally infected fish samples. However, granulomas reported by Huang et al.…”

“…Staphylococcal infections were reported in Turkey from cultured Oncorhynchus mykiss (Akayli et al., 2011; Metin et al., 2014; Timur & Akayli, 2003; Turgay, 2009), Sparus aurata (Canak & Akayli, 2013; Kubilay & Ulukoy, 2004; Yılmaz et al., 2019), Dicentrarchus labrax (Timur et al., 2008) and Dentex dentex (Akayli et al., 2011) mostly caused by S. epidermidis , S. aureus , S. hominis , S. cohnii and S. warneri .…”

Genetic characterization and antimicrobial profiling of bacterial isolates collected from Nile tilapia (Oreochromis niloticus) affected by summer mortality syndrome

“…Numerous Staphylococcus spp. including S. warneri , S. hominis , S. aureus , S. xylosus , and S. epidermidis have been reported to cause septicemic like symptoms in various fish species (Metin et al, 2014; Oh et al, 2019; Yilmaz et al, 2019). Staphylococcus spp.…”

Investigation of mass mortality caused by multi-drug resistant Edwardsiella tarda in Oscar fish, Astronotus ocellatus from an ornamental fish farm, Kerala

“…Still, S. hominis could be identi ed one among the Staphylococci species from Turkish aquaculture systems by showing signi cant clinical and histopathological changes in shes (Canak and Timur 2020). It was also found to be responsible for hyperplasia and hemorrhages in liver and kidney of cultured gilthead sea bream (Yilmaz et al 2019). Moreover, S. hominis could also found to cause cellulitis and bacteremia in humans and hence raise public concern while managing sh health (Uddin et al 2022).…”