Isolation of Specific Neuron Populations from Roundworm &lt;em&gt;Caenorhabditis elegans&lt;/em&gt;

Zahayko, Nataliya; Khalimonchuk, Oleh

doi:10.3791/60145

Cited by 2 publications

(2 citation statements)

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“…Non-coding RNAs and RNA binding proteins have emerged as key regulators in the nervous system. miRNAs (micro RNAs) modulate protein levels by typically binding to the 3′-untranslated region of cytosolic mRNA targets, triggering mRNA degradation ( Germany et al, 2019 ). miR-134, a brain-specific miRNA negatively modulates synapse development by inhibiting LIM kinase 1 protein translation ( Schratt et al, 2006 ).…”

Section: Broad Sense Translatomicsmentioning

confidence: 99%

“…However, it is especially important in the nervous system because it is relatively difficult to reliably obtain translational descriptions of specific cells from the central nervous system tissues due to its complex cellular heterogeneity ( Okada et al, 2011 ). Although Fluorescence Activated Cell Sorting (FACS) method has been able to isolate specific cells of brain and Schwann cells of the peripheral nervous system ( Rubio et al, 2016 ; Germany et al, 2019 ; Tomlinson et al, 2020 ), it takes one whole day to dissociate the tissue and isolate cells by FACS ( Rubio et al, 2016 ). Thus, it may change gene translational expression patterns and limit the utility of time-consuming translatomics methods including polysome profiling and ribosome profiling.…”

Section: Narrow Sense Translatomicsmentioning

confidence: 99%

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Insights Into Translatomics in the Nervous System

et al. 2020

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Most neurological disorders are caused by abnormal gene translation. Generally, dysregulation of elements involved in the translational process disrupts homeostasis in neurons and neuroglia. Better understanding of how the gene translation process occurs requires detailed analysis of transcriptomic and proteomic profile data. However, a lack of strictly direct correlations between mRNA and protein levels limits translational investigation by combining transcriptomic and proteomic profiling. The much better correlation between proteins and translated mRNAs than total mRNAs in abundance and insufficiently sensitive proteomics approach promote the requirement of advances in translatomics technology. Translatomics which capture and sequence the mRNAs associated with ribosomes has been effective in identifying translational changes by genetics or projections, ribosome stalling, local translation, and transcript isoforms in the nervous system. Here, we place emphasis on the main three translatomics methods currently used to profile mRNAs attached to ribosome-nascent chain complex (RNC-mRNA). Their prominent applications in neurological diseases including glioma, neuropathic pain, depression, fragile X syndrome (FXS), neurodegenerative disorders are outlined. The content reviewed here expands our understanding on the contributions of aberrant translation to neurological disease development.

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Section: Broad Sense Translatomicsmentioning

confidence: 99%