Isolation of secondary metabolites from Hortia oreadica (Rutaceae) leaves through high-speed counter-current chromatography

Target-guided isolation and purification of antioxidants from Selaginella sinensis by offline coupling of DPPH-HPLC and HSCCC experiments

“…Then, lately a simple and useful approach by spiking complex extract with DPPH test has been successfully developed [16,17]. As for preparative method, high-speed counter-current chromatography (HSCCC) is an optimal choice, which is a continuous liquid-liquid partition chromatography based on partitioning of compounds between two immiscible liquid phases without support matrix, no irreversible adsorption, low risk of sample denaturation, total sample recovery, large load capacity, and low cost [18], and has been successfully applied to isolate and purify many bioactive compounds from natural products [19][20][21][22]. In general, the split screening method and preparative means were disadvantageous to the fast isolating active compounds from TCM.…”

Chemical Constituents and Biological Activities of <i>Zanthoxylum limonella</i> (Rutaceae): A Review

“…Phytochemical survey of this family reveals alkaloids, coumarins, flavonoids, limonoids, and volatile oils [4]. Many of these compounds have been associated to different biological activities, for example, antimicrobial [3,5], antidiarrhoeal [6], anticholinesterasic [7], antileishmanial [8], antiprotozoal [9], larvicidal [10,11], and antioxidant activities [12,13]. In family Rutaceae, the genus Zanthoxylum has been able to provide a variety of secondary metabolites with interesting phytochemical and biological activities [14].…”

Preparative separation of glycoalkaloids α-solanine and α-chaconine by centrifugal partition chromatography

“…Moreover, the separation factor between any two components should be greater than 1.5 [17]. In our case, the ˛ value calculated from the K C preliminary extraction data set is 1.62, whereas the ˛ value obtained by the estimated values of Table 3 is 1.23.…”