Isolation of recombinant Hepatitis B surface antigen with antibody-conjugated superparamagnetic Fe3O4/SiO2 core-shell nanoparticles

Mostafaei, Mehdi; Hosseini, Seyed Nezamedin; Khatami, Maryam; Javidanbardan, Amin; Sepahy, Abbas Akhavan; Asadi, Ehsan

doi:10.1016/j.pep.2017.12.004

Cited by 41 publications

(20 citation statements)

References 26 publications

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“…In recent years, functionalized magnetic nanoparticles are successfully applied in bioseparation for cells such as cancer and bacterial cells, biological molecules such as proteins, peptides, and nucleic acids (Jain 2003;Lee et al 2018;Mostafaei et al 2018), and viruses (Li et al 2011;Zhao et al 2012). For the viruses, however, most applications focus on efficient and rapid detection.…”

Section: Discussionmentioning

confidence: 99%

Single-domain antibodies as promising experimental tools in imaging and isolation of porcine epidemic diarrhea virus

Yang

Yin

et al. 2018

Appl Microbiol Biotechnol

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Single-domain antibody (sdAb) or nanobody possesses specific features non-accessible for conventional antibodies that make them suitable for research and biotechnological applications. Porcine epidemic diarrhea virus (PEDV) causes lethal diarrhea in piglets, resulting in great economic losses all over the world. To detect and isolate PEDV rapidly and accurately is important for the control and further research of the clinical PEDV strains. In this study, four sdAb fragments (sdAb-Mc19/29/30/37) targeting the membrane (M) protein of PEDV were selected from sdAb library that was constructed through M protein-immunized Camelus bactrianus. The selected sdAb-Mcs were solubly expressed in Escherichia coli. The functional characteristics analysis revealed that the recombinant sdAb-Mcs have excellent binding activity and specificity to M protein but have no neutralizing activity to PEDV. For further application, sdAb-Mc37 was conjugated with quantum dots to synthesize a nanoprobe for imaging PEDV in vero cells. The observed fluorescence in vero cells clearly reflects that PEDV virions can be reliably recognized and labeled by the nanoprobe. Furthermore, the sdAb-Mc29 was conjugated with superparamagnetic nanobeads to construct immunomagnetic nanobeads (IMNBs) used to isolate PEDV. One PEDV strain was successfully isolated from clinical fecal sample, suggesting IMNBs as a novel and efficient tool suitable for PEDV isolation from clinical samples. This study provided a novel application and substantiated the suitability of sdAb as a specific binder for the isolation of viruses.

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Section: Discussionmentioning

confidence: 99%

Single-domain antibodies as promising experimental tools in imaging and isolation of porcine epidemic diarrhea virus

Yang

Yin

et al. 2018

Appl Microbiol Biotechnol

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“…In the last decade, magnetite nanoparticles (Fe 3 O 4 NPs) have attracted much attention due to biocompatibility and magnetic properties, and many studies have been done on their application . Thus, Fe 3 O 4 NPs with crystalline phase seem to be an important nanomaterial (NM) for many biomedical uses including cell therapy as cell labeling, as a tool for separate and purify cell populations, tissue repair, targeting, drug delivery, MRI, hyperthermia,, photocatalysis, ferro‐fluid technology, magneto‐caloric refrigeration, pigment, magnetic storage, magnetic ink printing, microwave absorption, biosensors, bioseparation and immune magnetic array …”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Fe₃O₄/Cu Nanocomposite: Green Synthesis Using Carum carvi L. Seeds Aqueous Extract

2019

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Here, we describe the using of biosynthesis method for the synthesis of Fe3O4, Cu nanoparticles and Fe3O4/Cu nanocomposite using Carum carvi extract and assessment of their antibacterial activity. Our approach is eco‐friendly, non‐toxic and clean. These nanoparticles/nanocomposites were characterized by UV‐Vis, FTIR, XRD, EDX and SEM, techniques. The findings indicated that the of Fe3O4 and Cu NPs, and Fe3O4/Cu nanocomposite have an average particle size of 25 nm, 37 nm, and 62 nm, respectively. On the other side, the Fe3O4 NPs do not have the antibacterial activity, but when they combine with Cu NPs with antibacterial activity, the Fe3O4/Cu nanocomposite shows the antimicrobial activity.

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“…This phenomenon indicated the successful synthesis of polymer and SiO 2 . Fe 3 O 4 , Fe 3 O 4 @SiO 2 , and FSMAA were almost spherical and had a certain degree of adhering owing to their nanometer size and high reactivity [17]. …”

Section: Resultsmentioning

confidence: 99%

Polymer-Functionalized Magnetic Nanoparticles: Synthesis, Characterization, and Methylene Blue Adsorption

Zheng

Zhao

et al. 2018

Materials

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The removal of methylene blue (MB) from wastewater has attracted global concerns. In this study, polymer-functionalized magnetic nanoparticles for MB removal, Fe3O4@SiO2-MPS-g-AA-AMPS (FSMAA), were successfully synthesized by grafting acrylic acid (AA) and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) on the surface of vinyl-modified Fe3O4@SiO2. With various characterization techniques, it was confirmed that the obtained FSMAA had a core–shell structure, a good magnetic property, and plenty of functional groups on its surface. MB adsorption experiments showed that the adsorption capacity of FSMAA was notably enhanced as the grafted monomer concentration and solution pH were increased. The adsorption kinetic data and isothermal data were well described by the pseudo-second-order kinetic model and the Langmuir model, respectively. The maximum adsorption capacity of FSMAA was 421.9 mg g−1 with grafted monomer concentration at 2.0 mol L−1 and solution pH at 9, much higher than those of other adsorbents stated in previous literatures. Based on XPS analysis, surface adsorption mechanism between FSMAA and MB was electrostatic interaction, hydrogen bonding, and hydrophobic interaction. Furthermore, FSMAA was effectively regenerated by acid pickling, and the remaining adsorption capacity was more than 60% after eight adsorption–regeneration cycles. All the results demonstrated the self-made FSMAA was a desirable adsorbent to remove MB from wastewater.

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Isolation of recombinant Hepatitis B surface antigen with antibody-conjugated superparamagnetic Fe3O4/SiO2 core-shell nanoparticles

Cited by 41 publications

References 26 publications

Single-domain antibodies as promising experimental tools in imaging and isolation of porcine epidemic diarrhea virus

Single-domain antibodies as promising experimental tools in imaging and isolation of porcine epidemic diarrhea virus

Antibacterial Activity of Fe₃O₄/Cu Nanocomposite: Green Synthesis Using Carum carvi L. Seeds Aqueous Extract

Polymer-Functionalized Magnetic Nanoparticles: Synthesis, Characterization, and Methylene Blue Adsorption

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Isolation of recombinant Hepatitis B surface antigen with antibody-conjugated superparamagnetic Fe3O4/SiO2 core-shell nanoparticles

Cited by 41 publications

References 26 publications

Single-domain antibodies as promising experimental tools in imaging and isolation of porcine epidemic diarrhea virus

Single-domain antibodies as promising experimental tools in imaging and isolation of porcine epidemic diarrhea virus

Antibacterial Activity of Fe3O4/Cu Nanocomposite: Green Synthesis Using Carum carvi L. Seeds Aqueous Extract

Polymer-Functionalized Magnetic Nanoparticles: Synthesis, Characterization, and Methylene Blue Adsorption

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Product

Resources

About

Antibacterial Activity of Fe₃O₄/Cu Nanocomposite: Green Synthesis Using Carum carvi L. Seeds Aqueous Extract