volume 55, issue 5, P1372-1379 1990
DOI: 10.1135/cccc19901372
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Abstract: Insulin extract has been prepared from rabbit disintegrated pancreas by means of acidic ethanol extraction in combination with ammonium acetate precipitation and purified by permeation chromatography. The enriched preparation of rabbit insulin has been fractionated further by affinity chromatography on AH-Sepharose 4B substituted with polylysine.