Isolation of pathogenic bacteria from sputum samples using a 3D-printed cartridge system

Abstract

Within this contribution we introduce a 3D-printed cartridge system enabling the convenient and cost-efficient sample preparation from sputum for subsequent PCR based detection schemes. The developed fluidic system operates is...

Progress in methods for the detection of viable Escherichia coli

“…Pahlow and colleagues developed a 3D printed cassette system that facilitated simple and cost-effective sample preparation for subsequent PCR-based assay protocols. 280 The amine-based functionalized particles facilitated the enrichment of intact and viable bacterial cells, including E. coli . Khanh and colleagues employed the MicroBio μ3D™ AutoScanner time-lapse shadow image system to track the progress of E. coli cells in varying temperature conditions.…”

3D Printing Technology: Role in Safeguarding Food Security

“…Beyond sensing capabilities, 3D printing offers the opportunity to fabricate additional structural components for analytical devices. A portable 3D printed chamber manufactured through an FDM technique is described to house the electrochemical components to perform multiple-pathogen identification , or specific bacteria screening for E. coli, S.…”

“…Beyond sensing capabilities, 3D printing offers the opportunity to fabricate additional structural components for analytical devices. A portable 3D printed chamber manufactured through an FDM technique is described to house the electrochemical components to perform multiple-pathogen identification 60 , 61 or specific bacteria screening for E. coli , 50 S. aureus , 52 cytotoxic food preservatives, 62 and Bacillus cereus . 41 Then, using a selective laser melting (SLM) printed compact microfluidic intestinal microvilli separator, it detects different concentrations of wheat allergen gliadin (range of 0.1–0.8 ng/mL).…”

Illuminating the Tiny World: A Navigation Guide for Proper Raman Studies on Microorganisms

“…The ability to isolate and identify bacteria provides us with a window into their complex world, allowing us to understand their ecological dynamics, genetic makeup, and impact on our lives, which has contributed to significant advances in food safety [ 7 , 96 , 152 , 155 , 156 ], medical diagnosis [ 8 , 14 , 20 , 59 , 130 , 157 , 158 ], the implementation of effective environmental preservation measures [ 159 , 160 , 161 , 162 ], and the revolution of industries through biotechnological advancements [ 163 , 164 , 165 , 166 , 167 ]. Contrary to the common perception of bacterial isolation as a simple task, the effectiveness of the isolation strategy for Raman spectroscopy analysis is dependent on a complex array of factors, including proper specimen collection, transport, storage, and processing.…”

“…Of course, other aspects such as (I) cell concentration in the sample [ 6 , 187 ]; (II) yield of cells [ 6 , 105 ]; and (III) minimizing any influence on the diversity within a bacterial species [ 186 ] are also important. To date, numerous studies have isolated bacteria from a wide range of matrices, from medically relevant media such as bronchoalveolar lavage (BAL) [ 105 ], ascitic fluid [ 94 ], blood [ 14 ], urine [ 188 ], and sputum [ 135 , 157 ] to consumables such as milk [ 155 , 186 ], meat [ 96 ], and feedstuff [ 125 ]. These featured various Raman-compatible isolation strategies of varying complexities that may be considered culture-dependent or culture-independent.…”