Isolation of Moderately Halophilic Bacteria in Saline Environments of Sonora State Searching for Proteolytic Hydrolases

Abstract

The aim of the study was to isolate moderately halophilic bacteria that produce proteolytic enzymes with industrial biotechnological value. Screening of halophiles from various saline habitats, led to the isolation of 210 moderately halophilic bacteria producing industrially important hydrolases; such as proteases, which are enzymes that hydrolyze the peptide bonds of proteins, represent one of the three groups of industrial enzymes. The present study of halophilic bacteria, producing proteases and isolated from different saline soils of Sonora State, was divided in two parts: the first part included screening of moderately halophiles bacteria collected from various regions, while the second part consisted of enzyme production by fermentation in liquid medium in flask. Three strains of bacteria with potential proteolytic activity (BLRMAM1066, BLCLAM1064, PPSE3AM1053) were selected to continue the electrophoresis and zymogram tests, obtaining molecular weights from 19 to 193 kDa. One of the a priori objectives of this research is to have a collection of halophilic bacteria with high proteolytic activity

Microbial Proteases Applications

“…Other cheap sources of substrate, such as cow dung, agro-industrial waste, groundnuts, and wheat bran can be remarkable for the production of proteases (Krishnaveni et al, 2012; Verma and Agarwa, 2016; Hamza, 2017a). Additionally, other readily available sources of substrate, such as molasses from sugar industry waste, dairy sludge, and effluents are interestingly promising for value-added product enzyme production and concurrently help to lessen eco-pollution (Prabhavathy et al, 2012; Chatterjee et al, 2015; Rao et al, 2017; Corral et al, 2018). For the commercial production of various enzymes especially proteases, waste from the agriculture industry is expected to be used in the future.…”

Production Assay and Partial Characterization of a Protease Produced by Idiomarina loihiensis, a Moderately Halophilic Bacterium Strain

“…Halophilic microbes isolated from different saline soils have been found to produce proteases with potential industrial biotechnological value [4]. They are most significant as their properties can be easily modified through genetic manipulations to suit their various applications [5].…”