Isolation of leishmanicidal triterpenes and lignans from the Amazonian lianaDoliocarpus dentatus (dilleniaceae)

Isolation of Cytotoxic Metabolites from Targeted Peruvian Amazonian Medicinal Plants

“…In northwestern Perú, the vine water of D. dentatus , known in the Aguaruna community as “waúna”, is used to treat aftereffects of malaria. A previous study of D. dentatus identified antileishmanial principles . In the present work, the bioassay-guided fractionation of the diethyl ether extract of D. dentatus led to the isolation in high yield (up to 53%) of betulinic acid ( 1 ) as the cytotoxic active metabolite.…”

“…A previous study of D. dentatus identified antileishmanial principles. 6 In the present work, the bioassay-guided fractionation of the diethyl ether extract of D. dentatus led to the isolation in high yield (up to 53%) of betulinic acid (1) as the cytotoxic active metabolite. The highest cytotoxicity for 1 was found against the K562 cell line (GI 50 ) 10.8 µg/mL, Table 1).…”

“…A previous study on I. juruensis identified antioxidant principles. 15 In the present work, the bioassayguided fractionation of the diethyl ether extract of I. juriensis led to the isolation of two dihydrochalcones, 2′,4′-dihydroxy-6′methoxy-3,4-methylenedioxydihydrochalcone (5) and 2′,4′-dihydroxy-4,6′-dimethoxydihydrochalcone (6), as the cytotoxic active metabolites. Their most potent cytotoxic activity was found against the K562 cell line (GI 50 ) 8.4 and 7.4 µg/mL, respectively).…”

Isolation of Cytotoxic Metabolites from Targeted Peruvian Amazonian Medicinal Plants

“…In northwestern Perú, the vine water of D. dentatus , known in the Aguaruna community as “waúna”, is used to treat aftereffects of malaria. A previous study of D. dentatus identified antileishmanial principles . In the present work, the bioassay-guided fractionation of the diethyl ether extract of D. dentatus led to the isolation in high yield (up to 53%) of betulinic acid ( 1 ) as the cytotoxic active metabolite.…”

“…A previous study of D. dentatus identified antileishmanial principles. 6 In the present work, the bioassay-guided fractionation of the diethyl ether extract of D. dentatus led to the isolation in high yield (up to 53%) of betulinic acid (1) as the cytotoxic active metabolite. The highest cytotoxicity for 1 was found against the K562 cell line (GI 50 ) 10.8 µg/mL, Table 1).…”

“…A previous study on I. juruensis identified antioxidant principles. 15 In the present work, the bioassayguided fractionation of the diethyl ether extract of I. juriensis led to the isolation of two dihydrochalcones, 2′,4′-dihydroxy-6′methoxy-3,4-methylenedioxydihydrochalcone (5) and 2′,4′-dihydroxy-4,6′-dimethoxydihydrochalcone (6), as the cytotoxic active metabolites. Their most potent cytotoxic activity was found against the K562 cell line (GI 50 ) 8.4 and 7.4 µg/mL, respectively).…”

Leishmanicidal Activity of Betulin Derivatives in Leishmania amazonensis; Effect on Plasma and Mitochondrial Membrane Potential, and Macrophage Nitric Oxide and Superoxide Production

“…In L. (V.) braziliensis promastigotes, the most potent compound was 26 (3,28-diformylbetulin triazolodione) with a GI 50 < 1 µM [29]. Likewise, a betulin aldehyde derivative reduced infection up to 88% at 136 µM and 58% at 68 µM of intracellular L. (L.) amazonensis amastigotes [63]. Herein, 26 was also within the initially selected compounds.…”

Exploring the Cytokinin Profile of Doliocarpus dentatus (Aubl.) Standl. From Guyana and Its Relationship with Secondary Metabolites: Insights into Potential Therapeutic Benefits

“…CKs in D. dentatus , such as t Z cZ and iPR, have shown promise in reducing oxidative stress, a factor in chronic diseases such as cancer and neurodegenerative disorders [ 51 , 132 , 133 ]. Traditional use of D. dentatus for conditions such as arthritis, diabetes, and gastrointestinal disorders supports its therapeutic potential, with the red ecotype showing higher CK concentrations and enhanced bioactivity compared to the white ecotype [ 13 , 25 , 130 , 131 , 134 , 135 , 136 ].…”

Isolation of Cytotoxic Metabolites from Targeted Peruvian Amazonian Medicinal Plants

“…In northwestern Perú, the vine water of D. dentatus , known in the Aguaruna community as “waúna”, is used to treat aftereffects of malaria. A previous study of D. dentatus identified antileishmanial principles . In the present work, the bioassay-guided fractionation of the diethyl ether extract of D. dentatus led to the isolation in high yield (up to 53%) of betulinic acid ( 1 ) as the cytotoxic active metabolite.…”

“…A previous study of D. dentatus identified antileishmanial principles. 6 In the present work, the bioassay-guided fractionation of the diethyl ether extract of D. dentatus led to the isolation in high yield (up to 53%) of betulinic acid (1) as the cytotoxic active metabolite. The highest cytotoxicity for 1 was found against the K562 cell line (GI 50 ) 10.8 µg/mL, Table 1).…”

“…A previous study on I. juruensis identified antioxidant principles. 15 In the present work, the bioassayguided fractionation of the diethyl ether extract of I. juriensis led to the isolation of two dihydrochalcones, 2′,4′-dihydroxy-6′methoxy-3,4-methylenedioxydihydrochalcone (5) and 2′,4′-dihydroxy-4,6′-dimethoxydihydrochalcone (6), as the cytotoxic active metabolites. Their most potent cytotoxic activity was found against the K562 cell line (GI 50 ) 8.4 and 7.4 µg/mL, respectively).…”

Leishmanicidal Activity of Betulin Derivatives in Leishmania amazonensis; Effect on Plasma and Mitochondrial Membrane Potential, and Macrophage Nitric Oxide and Superoxide Production

“…In L. (V.) braziliensis promastigotes, the most potent compound was 26 (3,28-diformylbetulin triazolodione) with a GI 50 < 1 µM [29]. Likewise, a betulin aldehyde derivative reduced infection up to 88% at 136 µM and 58% at 68 µM of intracellular L. (L.) amazonensis amastigotes [63]. Herein, 26 was also within the initially selected compounds.…”

Exploring the Cytokinin Profile of Doliocarpus dentatus (Aubl.) Standl. From Guyana and Its Relationship with Secondary Metabolites: Insights into Potential Therapeutic Benefits

“…CKs in D. dentatus , such as t Z cZ and iPR, have shown promise in reducing oxidative stress, a factor in chronic diseases such as cancer and neurodegenerative disorders [ 51 , 132 , 133 ]. Traditional use of D. dentatus for conditions such as arthritis, diabetes, and gastrointestinal disorders supports its therapeutic potential, with the red ecotype showing higher CK concentrations and enhanced bioactivity compared to the white ecotype [ 13 , 25 , 130 , 131 , 134 , 135 , 136 ].…”

Isolation of Cytotoxic Metabolites from Targeted Peruvian Amazonian Medicinal Plants

“…In northwestern Perú, the vine water of D. dentatus , known in the Aguaruna community as “waúna”, is used to treat aftereffects of malaria. A previous study of D. dentatus identified antileishmanial principles . In the present work, the bioassay-guided fractionation of the diethyl ether extract of D. dentatus led to the isolation in high yield (up to 53%) of betulinic acid ( 1 ) as the cytotoxic active metabolite.…”

“…A previous study of D. dentatus identified antileishmanial principles. 6 In the present work, the bioassay-guided fractionation of the diethyl ether extract of D. dentatus led to the isolation in high yield (up to 53%) of betulinic acid (1) as the cytotoxic active metabolite. The highest cytotoxicity for 1 was found against the K562 cell line (GI 50 ) 10.8 µg/mL, Table 1).…”

“…A previous study on I. juruensis identified antioxidant principles. 15 In the present work, the bioassayguided fractionation of the diethyl ether extract of I. juriensis led to the isolation of two dihydrochalcones, 2′,4′-dihydroxy-6′methoxy-3,4-methylenedioxydihydrochalcone (5) and 2′,4′-dihydroxy-4,6′-dimethoxydihydrochalcone (6), as the cytotoxic active metabolites. Their most potent cytotoxic activity was found against the K562 cell line (GI 50 ) 8.4 and 7.4 µg/mL, respectively).…”

Leishmanicidal Activity of Betulin Derivatives in Leishmania amazonensis; Effect on Plasma and Mitochondrial Membrane Potential, and Macrophage Nitric Oxide and Superoxide Production

“…In L. (V.) braziliensis promastigotes, the most potent compound was 26 (3,28-diformylbetulin triazolodione) with a GI 50 < 1 µM [29]. Likewise, a betulin aldehyde derivative reduced infection up to 88% at 136 µM and 58% at 68 µM of intracellular L. (L.) amazonensis amastigotes [63]. Herein, 26 was also within the initially selected compounds.…”

Exploring the Cytokinin Profile of Doliocarpus dentatus (Aubl.) Standl. From Guyana and Its Relationship with Secondary Metabolites: Insights into Potential Therapeutic Benefits

“…CKs in D. dentatus , such as t Z cZ and iPR, have shown promise in reducing oxidative stress, a factor in chronic diseases such as cancer and neurodegenerative disorders [ 51 , 132 , 133 ]. Traditional use of D. dentatus for conditions such as arthritis, diabetes, and gastrointestinal disorders supports its therapeutic potential, with the red ecotype showing higher CK concentrations and enhanced bioactivity compared to the white ecotype [ 13 , 25 , 130 , 131 , 134 , 135 , 136 ].…”