Isolation of <i>Malassezia sympodialis</i> and <i>Malassezia globosa</i> from healthy pet cats

Bond, R.; Howell, S. A.; Haywood, P. J.; Lloyd, D. H.

doi:10.1136/vr.141.8.200

Cited by 76 publications

(74 citation statements)

References 13 publications

(20 reference statements)

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“…Classically, lipid-dependent species have only been reported in association with human skin, and M. pachydermatis was considered the only species that could be isolated from animals. The presence of lipid-dependent species in carnivores was recently suggested by their presence on the ears and skin, initially in cats 4 and later in dogs. 9,33 The isolation of the lipid-dependent species from dogs in this study suggests a potential role of these animals as carriers for humans.…”

Section: Discussionmentioning

confidence: 99%

“…4 Although cytological, cultural, and histopathological techniques may be used to detect Malassezia yeasts, it has not yet been determined which of these techniques are most efficient. 18 The purpose of this study was to improve the knowledge of the most suitable diagnostic methodologies for Malassezia spp.…”

Section: Introductionmentioning

confidence: 99%

“…28,40 In recent years, some lipiddependent species such as M. furfur, M. globosa, and M. sympodialis also have been isolated from the ears and skin of healthy and diseased dogs and cats. 4,9,10,33 The isolation of lipid-dependent Malassezia species such as M. furfur has stimulated a controversial debate among mycologists because M. furfur had never been found in pet carnivores. 16 The frequency and population size of M. pachydermatis vary markedly among different anatomical sites in dogs.…”

Section: Introductionmentioning

confidence: 99%

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Frequency, Body Distribution, and Population Size of Malassezia Species in Healthy Dogs and in Dogs with Localized Cutaneous Lesions

et al. 2005

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Abstract. Malassezia species are commensal organisms of human and animal skin that occasionally act as opportunistic pathogens. The lipid-dependent species are associated with human skin disorders, whereas the non-lipid-dependent species (Malassezia pachydermatis) is considered as an opportunistic secondary pathogen affecting the canine skin surface and ear canal. This study evaluated the relationship between Malassezia yeasts, their population size, and the occurrence of skin lesions from healthy and skin-diseased dogs. The efficiency of cytological examination and fungal culture for Malassezia detection was also evaluated. From March 2002 to July 2003, 33 healthy dogs and 54 dogs with pruritic localized skin diseases were examined; skin swabs (1218) were collected from 7 anatomical sites for culture and cytological examination. Malassezia prevalence according to anatomical site and the agreement between cytological results and fungal cultures were statistically analyzed. Differences in mean colony forming unit counts between positive healthy and diseased dogs were evaluated using the Bonferroni test for post hoc pair-wise comparisons. In healthy dogs, Malassezia yeasts were most frequently isolated in the perianal and perioral areas. The frequency of isolation and population size of Malassezia species were higher in dogs with localized dermatitis, especially in affected areas, indicating a role for Malassezia in the occurrence of skin lesions. Malassezia pachydermatis was the species most commonly cultured from the skin and external ear canal of healthy and diseased dogs; isolation of lipid-dependent yeasts from healthy dogs was less frequent. Using fungal culture as the gold standard, cytological examination showed good relative specificity (95%) but very low relative sensitivity (30%).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Frequency, Body Distribution, and Population Size of Malassezia Species in Healthy Dogs and in Dogs with Localized Cutaneous Lesions

et al. 2005

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“…The above investigations have shown that not only M. pachydermatis but also other, lipophilic species are inhabitants of the ear canal and skin microflora of domestic animals (Bond et al, 1996a;Bond et al, 1997;Crespo et al, 2002;Cafarchia et al, 2005;Nardoni et al, 2005). This fact is also suggested in relation to wild felids, since M. sympodialis was isolated from the microflora of the external ear canal in 33 out of 55 (56.9%) large felids studied (Coutinho et al, 2006).…”

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confidence: 79%

Isolation of Malassezia pachydermatis and M. sympodialis from the external ear canal of cats with and without otitis externa

Dizotti

Coutinho

2007

Acta Veterinaria Hungarica

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The objective of this study was to determine the presence of Malassezia spp. in the external ear canal of cats with and without otitis. Forty-five animals were studied, 20 with and 25 without otitis externa (OE). Cerumen or secretion from external ear canal samples was cultured on modified Mycosel agar and sterile olive oil was added to the surface of the medium before specimen seeding. The isolates were analysed for macro-and micromorphology and identified by catalase tests and on the basis of growth on Tween 20, 40, 60 and 80. Malassezia spp. were isolated from 15 out of 20 (75%) animals with otitis and from 7 out of 25 (28%) cats without OE; the difference between the two groups was statistically significant (P ≤ 0.05). Malassezia pachydermatis and M. sympodialis were isolated from 60% (12/20) and 40% (8/20) of cats with otitis, respectively, with no significant difference in the frequency of isolation between the two species. In the microflora of the healthy ear canal M. pachydermatis was significantly more common (6/25, 24%) than M. sympodialis (1/25, 4%). The present investigation confirms that M. sympodialis can also act as an aetiological agent of feline OE, and if commercial veterinary laboratories do not use media with added lipids for the isolation of Malassezia spp., this might lead to false-negative results.

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“…In our study, Malassezia species positive cultures were obtained from 95.1% of diseased animals and 48.4% of healthy cats, indicating significant difference between them. Data available in literature showed prevalence ranging from less than 10% to 23% in healthy subjects [3,13,16,26], and from 19% to 41.2% in animals affected by otitis [9,13]. The higher frequency of Malassezia yeasts reported in animals with otitis compared to healthy animals indicates that these yeasts overgrow in infection sites and play a role in the pathogenesis of otitis externa.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Malassezia Species in Persian and Domestic Short Hair Cats with and without Otitis Externa

et al. 2010

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ABSTRACT. The yeasts of the Malassezia genus are opportunistic microorganisms in the skin and auricular canal of human and animals, mainly cats, and can cause otitis externa and dermatitis disorders. The aim of this study was to evaluate the occurrence of different species of Malassezia in the external ear canal of cats with and without otitis externa. Thirty-one normal cats and 82 animals with otitis externa were clinically examined. Sterile cotton swabs were used to collect specimens from the external ear canal and streaked onto the surface of Sabouraud dextrose agar (SDA) and modified Dixon agar. Malassezia yeasts were isolated from 95.1% and 48.4% of the cats with and without otitis externa, respectively. The rate of isolation in affected animals versus normals was highly significant (P<0.05). Out of the 137 isolates obtained from cats with otitis, 57.7% were identified as M. pachydermatis (with significant frequency; P<0.05), 15.4% as M. obtusa, 11.4% as M. globosa, 7.3% as M. slooffiae, 4.1% as M. sympodialis, 2.4% as M. furfur and 1.6% as M. restricta. Malassezia species were frequently isolated from subjects with age range from 1to 4 years old (42.7%). Our finding of Malassezia isolates indicated that feline otitis externa can be associated with lipid-dependent Malassezia species in addition to the non lipid-dependent species M. pachydermatis.

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Isolation of Malassezia sympodialis and Malassezia globosa from healthy pet cats

Cited by 76 publications

References 13 publications

Frequency, Body Distribution, and Population Size of Malassezia Species in Healthy Dogs and in Dogs with Localized Cutaneous Lesions

Frequency, Body Distribution, and Population Size of Malassezia Species in Healthy Dogs and in Dogs with Localized Cutaneous Lesions

Isolation of Malassezia pachydermatis and M. sympodialis from the external ear canal of cats with and without otitis externa

Occurrence of Malassezia Species in Persian and Domestic Short Hair Cats with and without Otitis Externa

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