Isolation of
            <i>Acidaminococcus fermentans</i>
            and
            <i>Megasphaera elsdenii</i>
            from Normal Human Feces

Sugihara, Paul T.; Sutter, Vera L.; Attebery, Howard R.; Bricknell, Kenneth S.; Finegold, Sydney M.

doi:10.1128/am.27.1.274-275.1974

Cited by 15 publications

(3 citation statements)

References 6 publications

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“…No other ASVs classified as M. elsdenii. The prevalence of M. elsdenii 4415 among the 30 microbiomes considered for this study (10%) was similar to previous reports on the occurrence of M. elsdenii in human stool (Sugihara et al, 1974). The abundance of M. elsdenii 4415 varied from 1.06% to 4.04% among the Me_D microbiomes (2.59 ± 0.86%; Figure 1A).…”

Section: Resultssupporting

confidence: 91%

Megasphaera elsdenii, a commensal member of the gut microbiota, is associated with elevated gas production during in vitro fermentation

Mutuyemungu,

Motta-Romero,

Yang

et al. 2023

Gut. Microb.

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Megasphaera elsdenii has been correlated with gas production by human faecal microbiota during fermentation. The objective of this study was to determine the role of M. elsdenii in gas production by the microbiome. Kidney beans and sweet potatoes were subjected to in vitro digestion and dialysis followed by fermentation with ten faecal microbiomes: three with detectable M. elsdenii (Me_D) and seven with no detectable M. elsdenii (Me_ND). Me_D microbiomes produced more gas than the Me_ND microbiomes (p < 0.001). Me_D microbiomes produced more gas during fermentation of sweet potatoes than kidney beans (p < 0.001), while the opposite was true for the Me_ND microbiomes (p < 0.001). Among amplicon sequence variants that were associated with gas production, M. elsdenii had the strongest association (p < 0.001). Me_D microbiomes consumed more acetate and produced more butyrate than Me_ND microbiomes (p < 0.001). Gas production by M. elsdenii was confirmed by fermentation of sweet potatoes and acetate with human and rumen M. elsdenii isolates. The human isolate produced gas on sweet potatoes and acetate. This study suggests that M. elsdenii may be involved in gas production during the fermentation of flatulogenic foods through utilisation of undigestible substrates or cross-feeding on acetate.

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Section: Resultssupporting

confidence: 91%

Megasphaera elsdenii, a commensal member of the gut microbiota, is associated with elevated gas production during in vitro fermentation

Mutuyemungu,

Motta-Romero,

Yang

et al. 2023

Gut. Microb.

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“…M. elsdenii is a Gram-negative, strictly anaerobic, coccus-shaped, nonmotile bacterium that inhabits the rumen of cattle and other ruminants [ 7 ] as well as the large intestine of humans [ 9 ] and pigs [ 10 ]. It is classified within the phylum Firmicutes (syn.…”

Section: Classification and Morphologymentioning

confidence: 99%

Megasphaera elsdenii: Its Role in Ruminant Nutrition and Its Potential Industrial Application for Organic Acid Biosynthesis

Cabral,

Weimer

2024

Microorganisms

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The Gram-negative, strictly anaerobic bacterium Megasphaera elsdenii was first isolated from the rumen in 1953 and is common in the mammalian gastrointestinal tract. Its ability to use either lactate or glucose as its major energy sources for growth has been well documented, although it can also ferment amino acids into ammonia and branched-chain fatty acids, which are growth factors for other bacteria. The ruminal abundance of M. elsdenii usually increases in animals fed grain-based diets due to its ability to use lactate (the product of rapid ruminal sugar fermentation), especially at a low ruminal pH (<5.5). M. elsdenii has been proposed as a potential dietary probiotic to prevent ruminal acidosis in feedlot cattle and high-producing dairy cows. However, this bacterium has also been associated with milk fat depression (MFD) in dairy cows, although proving a causative role has remained elusive. This review summarizes the unique physiology of this intriguing bacterium and its functional role in the ruminal community as well as its role in the health and productivity of the host animal. In addition to its effects in the rumen, the ability of M. elsdenii to produce C2–C7 carboxylic acids—potential precursors for industrial fuel and chemical production—is examined.

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“…At the time of writing, only five species of the genus Megasphaera have been described (Rogosa 1971; Rainey 2009). The type species Megasphaera elsdenii (Marchandin et al , 2009) is a normal inhabitant of the rumen of cattle and sheep and is also found in the faeces and intestine of humans and pigs (Gutierrez et al , 1959; Sugihara et al , 1974; Hino et al , 1994; Hashizume et al , 2003; Gill et al , 2006; Marchandin et al , 2009; Faith et al , 2013), it also produces hydrogen (Ohnishi et al , 2010). Megasphaera cerevisiae , Megasphaera paucivorans , and Megasphaera sueciensis are brewery-associated species (Engelmann & Weiss, 1985; Juvonen & Suihko, 2006), while Megasphaera micronuciformis was recovered from a human liver abscess and a pus sample (Marchandin et al , 2003).…”

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confidence: 99%

Megasphaera indica sp. nov., an obligate anaerobic bacteria isolated from human faeces

Lanjekar

Marathe

Ramana

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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DNA-DNA relatedness with NMBHI-10 T and BLPYG-7, respectively), but belong to the same species (DNA-DNA relatedness of 80.9 % between the isolates). According to DNA-DNA hybridization results, the coccoid strains belong to the same genospecies, and neither is related to any of the recognized species of the genus Megasphaera. Strains NMBHI-10 T and BLPYG-7 grew in PYG broth at temperatures of between 15 and 40 6C (optimum 37 6C), but not at 45 6C.The strains utilized a range of carbohydrates as sources of carbon and energy including glucose, lactose, cellobiose, rhamnose, galactose and sucrose. Glucose fermentation resulted in the formation of volatile fatty acids, mainly caproic acid and organic acids such as succinic acid. Phylogenetic analysis, specific phenotypic characteristics and/or DNA G+C content also differentiated the strains from each other and from their closest relatives. The DNA G+C contents of strains NMBHI-10 T and BLPYG-7 are 57.7 and 54.9 mol%, respectively. The major fatty acids were 12 : 0 FAME and 17 : 0 CYC FAME. On the basis of these data, we conclude that strains NMBHI-10 T and BLPYG-7 should be classified as representing a novel species of the genus Megasphaera, for which the name Megsphaera indica sp. nov. is proposed. The type strain is NMBHI-10 T (5DSM 25563 T 5MCC 2481 T ).The genus Megasphaera belongs to the family Veillonellaceae, sub-branch Sporomusa, class Clostridia, phylum Firmicutes and comprises Gram-stain-negative, obligately anaerobic bacteria (Marchandin et al., 2009). At the time of writing, only five species of the genus Megasphaera have been described (Rogosa 1971;Rainey 2009). The type species Megasphaera elsdenii (Marchandin et al., 2009) is a normal inhabitant of the rumen of cattle and sheep and is also found in the faeces and intestine of humans and pigs (Gutierrez et al., 1959; Sugihara et al., 1974; Hino et al., 1994; Hashizume et al., 2003;Gill et al., 2006;Marchandin et al., 2009;Faith et al., 2013), it also produces hydrogen (Ohnishi et al., 2010). Megasphaera cerevisiae, Megasphaera paucivorans, and Megasphaera sueciensis are breweryassociated species (Engelmann & Weiss, 1985;Juvonen & Suihko, 2006), while Megasphaera micronuciformis was recovered from a human liver abscess and a pus sample (Marchandin et al., 2003).In the present study two strains, designated NMBHI-10 T and BLPYG-7, belonging to the genus Megasphaera have been characterized. Detailed taxonomic analysis indicated that these strains represent a novel species of the genus Megasphaera.Strain NMBHI-10 T was isolated (from the faeces of a healthy 26-year-old male residing in Pune, India) on peptone-yeast extract-glucose (PYG) agar (Holdeman & Moore, 1977) at 37 u C after 48 h in an anaerobic chamber (Anaerobic system Abbreviations: TCD, thermal conductivity detector; VFA, volatile fatty acids; FID, Flame ionisation detector.The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains T and BLPYG-7 are HM990965 and HM990964, respectively.Two supplementary tables are availa...

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Isolation of Acidaminococcus fermentans and Megasphaera elsdenii from Normal Human Feces

Abstract: Fecal bacterial cultures from 40 normal humans yielded Megasphaera elsdenii from four individuals and Acidaminococcus fermentans from 10 individuals, with two individuals having both organisms.

Cited by 15 publications

References 6 publications

Megasphaera elsdenii, a commensal member of the gut microbiota, is associated with elevated gas production during in vitro fermentation

Megasphaera elsdenii, a commensal member of the gut microbiota, is associated with elevated gas production during in vitro fermentation

Megasphaera elsdenii: Its Role in Ruminant Nutrition and Its Potential Industrial Application for Organic Acid Biosynthesis

Megasphaera indica sp. nov., an obligate anaerobic bacteria isolated from human faeces

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