Isolation of hyperglycaemic peptides from the corpus cardiacum of the american cockroach, Periplaneta americana

Digestion-Free Analysis of Peptides from 30-year-old Formalin-Fixed, Paraffin-Embedded Tissue by Mass Spectrometry Imaging

“…Products from all these neuropeptide precursors are detected throughout the CCs tissue (Figure b), including leucomyosuppressin (Pea-LMS), corazonin, the sulfakinins Pea-SK and leucosulfakinin (Pea-LSK-II), kinin (Pea-K-1), and a range of pyrokinins (Pea-PK-1 to Pea-PK-6). The CCg region spectrum (Figure c) is dominated by the American cockroach cardioacceleratory hormones I and II (Pea-CAH-1 and Pea-CAH-2) . These two hypertrehalosemic hormones are readily detected as Na + adducts at m / z 995.4346 and 1010.4709, respectively, an observation also made in previous studies. , …”

“…The CCg region spectrum ( Figure 1 c) is dominated by the American cockroach cardioacceleratory hormones I and II (Pea-CAH-1 and Pea-CAH-2). 33 These two hypertrehalosemic hormones are readily detected as Na + adducts at m / z 995.4346 and 1010.4709, respectively, an observation also made in previous studies. 18 , 27 …”

“…The CCg region spectrum (Figure 1c) is dominated by the American cockroach cardioacceleratory hormones I and II (Pea-CAH-1 and Pea-CAH-2). 33 These two hypertrehalosemic hormones are readily detected as Na + adducts at m/z 995.4346 and 1010.4709, respectively, an observation also made in previous studies. 18,27 As illustrated by representative on-tissue, single-pixel mass spectra of the various regions of the retrocerebral complex (Figure 1), many of the observed peaks were detected at low relative abundances, being present at tissue (sub)structures, i.e., specific neurosecretory nerve fiber bundles and neuroendocrine cell groups.…”

Digestion-Free Analysis of Peptides from 30-year-old Formalin-Fixed, Paraffin-Embedded Tissue by Mass Spectrometry Imaging

“…Products from all these neuropeptide precursors are detected throughout the CCs tissue (Figure b), including leucomyosuppressin (Pea-LMS), corazonin, the sulfakinins Pea-SK and leucosulfakinin (Pea-LSK-II), kinin (Pea-K-1), and a range of pyrokinins (Pea-PK-1 to Pea-PK-6). The CCg region spectrum (Figure c) is dominated by the American cockroach cardioacceleratory hormones I and II (Pea-CAH-1 and Pea-CAH-2) . These two hypertrehalosemic hormones are readily detected as Na + adducts at m / z 995.4346 and 1010.4709, respectively, an observation also made in previous studies. , …”

“…The CCg region spectrum ( Figure 1 c) is dominated by the American cockroach cardioacceleratory hormones I and II (Pea-CAH-1 and Pea-CAH-2). 33 These two hypertrehalosemic hormones are readily detected as Na + adducts at m / z 995.4346 and 1010.4709, respectively, an observation also made in previous studies. 18 , 27 …”

“…The CCg region spectrum (Figure 1c) is dominated by the American cockroach cardioacceleratory hormones I and II (Pea-CAH-1 and Pea-CAH-2). 33 These two hypertrehalosemic hormones are readily detected as Na + adducts at m/z 995.4346 and 1010.4709, respectively, an observation also made in previous studies. 18,27 As illustrated by representative on-tissue, single-pixel mass spectra of the various regions of the retrocerebral complex (Figure 1), many of the observed peaks were detected at low relative abundances, being present at tissue (sub)structures, i.e., specific neurosecretory nerve fiber bundles and neuroendocrine cell groups.…”

Elucidation of the primary structures of the cockroach hyperglycaemic hormones I and II using enzymatic techniques and gas‐phase sequencing

“…The isolation procedure reported recently revealed two peptides, H G H I and HGHII, both of which were found to elicit a hyperglycaemic and phosphorylase-activating response when injected into female cockroaches (Siegert et al, 1986). These peptides are present in the CC of P.americana in a 3: 1 ratio.…”

Hypertrehalosaemic activity in corpus cardiacum‐corpus allatum‐aorta complex and adipokinetic response of Glossina morsitans

“…These granules are known to contain neurohormones, some of which control metabolic and reproductive processes (Raabe, 1982). The metabolic hormones are mainly the hypertrehalosaemic hormones which control the conversion of tissue glycogen into haemolymph trehalose (Goldsworthy , 1969;Scarborough et al, 1984;O'Shea el al., 1984;Siegert et al, 1986), and the adipokinetic hormones which cause the mobilization of fat body triacylglycerol to haemolymph diacylglycerol (Mayer & Candy, 1969;Stone et al, 1976;Carlsen et al, 1979). The existence of a hypertrehalosaemic neurohormone in the CC of Periplaneta was first noted by Steele (1961).…”

Digestion-Free Analysis of Peptides from 30-year-old Formalin-Fixed, Paraffin-Embedded Tissue by Mass Spectrometry Imaging

“…Products from all these neuropeptide precursors are detected throughout the CCs tissue (Figure b), including leucomyosuppressin (Pea-LMS), corazonin, the sulfakinins Pea-SK and leucosulfakinin (Pea-LSK-II), kinin (Pea-K-1), and a range of pyrokinins (Pea-PK-1 to Pea-PK-6). The CCg region spectrum (Figure c) is dominated by the American cockroach cardioacceleratory hormones I and II (Pea-CAH-1 and Pea-CAH-2) . These two hypertrehalosemic hormones are readily detected as Na + adducts at m / z 995.4346 and 1010.4709, respectively, an observation also made in previous studies. , …”

“…The CCg region spectrum ( Figure 1 c) is dominated by the American cockroach cardioacceleratory hormones I and II (Pea-CAH-1 and Pea-CAH-2). 33 These two hypertrehalosemic hormones are readily detected as Na + adducts at m / z 995.4346 and 1010.4709, respectively, an observation also made in previous studies. 18 , 27 …”

“…The CCg region spectrum (Figure 1c) is dominated by the American cockroach cardioacceleratory hormones I and II (Pea-CAH-1 and Pea-CAH-2). 33 These two hypertrehalosemic hormones are readily detected as Na + adducts at m/z 995.4346 and 1010.4709, respectively, an observation also made in previous studies. 18,27 As illustrated by representative on-tissue, single-pixel mass spectra of the various regions of the retrocerebral complex (Figure 1), many of the observed peaks were detected at low relative abundances, being present at tissue (sub)structures, i.e., specific neurosecretory nerve fiber bundles and neuroendocrine cell groups.…”

Elucidation of the primary structures of the cockroach hyperglycaemic hormones I and II using enzymatic techniques and gas‐phase sequencing

“…The isolation procedure reported recently revealed two peptides, H G H I and HGHII, both of which were found to elicit a hyperglycaemic and phosphorylase-activating response when injected into female cockroaches (Siegert et al, 1986). These peptides are present in the CC of P.americana in a 3: 1 ratio.…”

Hypertrehalosaemic activity in corpus cardiacum‐corpus allatum‐aorta complex and adipokinetic response of Glossina morsitans

“…These granules are known to contain neurohormones, some of which control metabolic and reproductive processes (Raabe, 1982). The metabolic hormones are mainly the hypertrehalosaemic hormones which control the conversion of tissue glycogen into haemolymph trehalose (Goldsworthy , 1969;Scarborough et al, 1984;O'Shea el al., 1984;Siegert et al, 1986), and the adipokinetic hormones which cause the mobilization of fat body triacylglycerol to haemolymph diacylglycerol (Mayer & Candy, 1969;Stone et al, 1976;Carlsen et al, 1979). The existence of a hypertrehalosaemic neurohormone in the CC of Periplaneta was first noted by Steele (1961).…”

Digestion-Free Analysis of Peptides from 30-year-old Formalin-Fixed, Paraffin-Embedded Tissue by Mass Spectrometry Imaging

“…Products from all these neuropeptide precursors are detected throughout the CCs tissue (Figure b), including leucomyosuppressin (Pea-LMS), corazonin, the sulfakinins Pea-SK and leucosulfakinin (Pea-LSK-II), kinin (Pea-K-1), and a range of pyrokinins (Pea-PK-1 to Pea-PK-6). The CCg region spectrum (Figure c) is dominated by the American cockroach cardioacceleratory hormones I and II (Pea-CAH-1 and Pea-CAH-2) . These two hypertrehalosemic hormones are readily detected as Na + adducts at m / z 995.4346 and 1010.4709, respectively, an observation also made in previous studies. , …”

“…The CCg region spectrum ( Figure 1 c) is dominated by the American cockroach cardioacceleratory hormones I and II (Pea-CAH-1 and Pea-CAH-2). 33 These two hypertrehalosemic hormones are readily detected as Na + adducts at m / z 995.4346 and 1010.4709, respectively, an observation also made in previous studies. 18 , 27 …”

“…The CCg region spectrum (Figure 1c) is dominated by the American cockroach cardioacceleratory hormones I and II (Pea-CAH-1 and Pea-CAH-2). 33 These two hypertrehalosemic hormones are readily detected as Na + adducts at m/z 995.4346 and 1010.4709, respectively, an observation also made in previous studies. 18,27 As illustrated by representative on-tissue, single-pixel mass spectra of the various regions of the retrocerebral complex (Figure 1), many of the observed peaks were detected at low relative abundances, being present at tissue (sub)structures, i.e., specific neurosecretory nerve fiber bundles and neuroendocrine cell groups.…”

Elucidation of the primary structures of the cockroach hyperglycaemic hormones I and II using enzymatic techniques and gas‐phase sequencing

“…The isolation procedure reported recently revealed two peptides, H G H I and HGHII, both of which were found to elicit a hyperglycaemic and phosphorylase-activating response when injected into female cockroaches (Siegert et al, 1986). These peptides are present in the CC of P.americana in a 3: 1 ratio.…”

Hypertrehalosaemic activity in corpus cardiacum‐corpus allatum‐aorta complex and adipokinetic response of Glossina morsitans

“…These granules are known to contain neurohormones, some of which control metabolic and reproductive processes (Raabe, 1982). The metabolic hormones are mainly the hypertrehalosaemic hormones which control the conversion of tissue glycogen into haemolymph trehalose (Goldsworthy , 1969;Scarborough et al, 1984;O'Shea el al., 1984;Siegert et al, 1986), and the adipokinetic hormones which cause the mobilization of fat body triacylglycerol to haemolymph diacylglycerol (Mayer & Candy, 1969;Stone et al, 1976;Carlsen et al, 1979). The existence of a hypertrehalosaemic neurohormone in the CC of Periplaneta was first noted by Steele (1961).…”