Isolation of hesperidin from peels of thinned Citrus unshiu fruits by microwave-assisted extraction

Inoue, Tsuyoshi; Tsubaki, Shuntaro; Ogawa, Kazunori; Onishi, Kiyotaka; Azuma, Jun-ichi

doi:10.1016/j.foodchem.2010.04.051

Cited by 118 publications

(64 citation statements)

References 27 publications

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“…The 13 C NMR also confirmed the compound to be hesperidin. The above physical and spectroscopy data were comparable with those of hesperidin reported in the literature with 2S configuration (Inove et al, 2010;Maltese et al, 2009).…”

Section: Discussionsupporting

confidence: 89%

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Bioassay guided fractionation and identification of active anti-inflammatory constituent fromDelonix elataflowers using RAW 264.7 cells

Saravanan

Ibrahim

David³

et al. 2014

Pharmaceutical Biology

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Context: Delonix elata (L.) Gamble (Fabaceae) has been used in the Indian traditional medicine system to treat rheumatism and inflammation. Aim: To assess the anti-inflammatory effect of Delonix elata flowers and to isolate the active principle. Materials and methods: The prompt anti-inflammatory constituent was isolated from Delonix elata flower extracts using bioassay guided fractionation in liposaccharide (LPS) stimulated RAW 264.7 macrophage cell line. The anti-inflammatory activity of extracts/fractions/subfractions/compounds (10, 25, and 50 mg/ml) was evaluated by estimating the levels of nitric oxide (NO), TNF-a, and IL-1b after 24 h of LPS induction (1 mg/ml). The isolated active compound was subjected to NMR, IR, and UV analyses for structure determination. Results: In an attempt to search for anti-inflammatory constituents, the active pure principle was isolated and crystallized as a white compound from Delonix elata flowers methanol extract. This active compound (50 mg/ml) decreased the release of inflammatory mediators levels such as NO (0.263 ± 0.03 mM), TNFa (160.20 ± 17.57 pg/ml), and IL-1b (285.79 ± 15.16 pg/ml) significantly (p50.05); when compared to the levels of NO (0.774 ± 0.08 mM), TNFa (501.71 ± 25.14 pg/ml), and IL-1b (712.68 ± 52.25 pg/ml) from LPS-stimulated macrophage cells. The active compound was confirmed as hesperidin with NMR, IR, and UV spectroscopy data. This is the first report of this compound from Delonix elata flowers. Conclusion: The findings of the study support the traditional use of Delonix elata flowers to treat inflammation.

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Section: Discussionsupporting

confidence: 89%

“…The NMR, IR, and UV (Figure 4a-e) analysis confirmed the compound to be hesperidin ( Figure 5) (Lin et al, 2007). The spectroscopy data were comparable with those of hesperidin reported in the literature with 2S configuration (Inove et al, 2010;Maltese et al, 2009). This is the first report of this compound from this plant.…”

supporting

confidence: 82%

Bioassay guided fractionation and identification of active anti-inflammatory constituent fromDelonix elataflowers using RAW 264.7 cells

Saravanan

Ibrahim

David³

et al. 2014

Pharmaceutical Biology

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“…It has been isolated at large scale from the rinds of paradisi Macfad., Citrus sinensis (L.) Osbeck, and Citrus unshiu Marcovitch. (Barthe et al, 1988;Inoue et al, 2010;Rouseff et al, 1987). It has also been reported from other Citrus species such as Citrus aurantium L. var.…”

Section: Food Sourcesmentioning

confidence: 78%

Hesperidin: A promising anticancer agent from nature

Devi

Rajavel

Nabavi

et al. 2015

Industrial Crops and Products

126

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“…The quantities of physiological drop of citrus fruits are quite considerable (Mao 2009). In China, only small quantities of physiological fruit drop are collected for use in traditional Chinese medicines (TCM) (Chinese Pharmacopoeia Edition Committee 2005), and in Japan, most of them are burned as an agricultural waste (Inoue and others 2010). However, immature citrus fruits usually have higher contents of phytochemical compounds than mature fruits (Barreca and others 2010; Inafuku‐Teramoto and others 2011).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Profile and Antioxidant Activity of Physiological Drop of Citrus Fruits

Sun

Liao

Shen

et al. 2013

Journal of Food Science

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Isolation of hesperidin from peels of thinned Citrus unshiu fruits by microwave-assisted extraction

Cited by 118 publications

References 27 publications

Bioassay guided fractionation and identification of active anti-inflammatory constituent fromDelonix elataflowers using RAW 264.7 cells

Bioassay guided fractionation and identification of active anti-inflammatory constituent fromDelonix elataflowers using RAW 264.7 cells

Hesperidin: A promising anticancer agent from nature

Phytochemical Profile and Antioxidant Activity of Physiological Drop of Citrus Fruits

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