Isolation of extracellular protein from greenhouse soil

A rapid isolation method of extracellular proteins produced by Pseudomonad strains

“…These results suggested that the biomolecules which were release by the bacterial cells isolated by filter sterilization of culture through 0.4mm filter for removing bacterial cells, however similar observation was previously reported in greenhouse soil which was filter sterilized through No. 6 filter paper which is not valuable for removal of bacterial cells (Matsumoto et al, 2000;Murase et al, 2003) and bacterial culture through 0.4mm filter (Sharma and Sharma, 2015a). Extracellular proteins might be playing an important role in the microbial environment likewise as in nitrogen storage and for microbial growth etc.…”

“…Microbial growth and development depends on the environment which is created in the media due to the presence of extracellular biomolecules. These biomolecules are difficult to isolate because they are immediately degraded by different factors (Murase et al, 2003). These enzymes may be recognized only by some indirect methods like enzyme assay, but still no reports have been reported for quantification.…”

Identification of proteins involved in starch and polygalacturonic acid degradation using LC/MS

“…Extraction of total protein was performed according to Murase et al [12] with some modifications. Namely, soil and compost samples were suspended in a 67 mM L -1 phosphate buffer (pH 6.0) and sonicated with constant cooling.…”

Bt protein rhizosecreted from transgenic maize does not accumulate in soil

“…The pH of the extraction buffer was 7. Neutral and slightly acidic pH values reduce the amount of humic acid and organic matter in the extracts (Murase et al 2003) and also the solubility of the native Bt crystal toxin present in soil.…”