Isolation of Enteropathogenic <italic>Escherichia coli</italic> (EPEC) From Raw Milk in Kermanshah by Polymerase Chain Reaction (PCR)

Abstract

Background: Infectious diarrhoeal diseases cause major problems throughout the world and are responsible for considerable morbidity and mortality. Enteropathogenic Escherichia coli (EPEC) may cause infantile diarrhoea among children in developing countries. Objectives: The aim of this study was to define the prevalence of EPEC strains in raw milk samples. Materials and Methods: Raw milk samples collected from various cow farms in Kermanshah, Iran, during the period of 22nd June to 22nd September 2009 and were examined for EPEC presence using PCR reactions targeting eaeA, and then stx1 and stx2. Results: Of the 206 samples, 17 (8.25%) were contaminated with E. coli eaeA positive and stx1 and stx2 negative (EPEC). Conclusions: Our results confirm that raw milk recovered in Kermanshah may be a source for gastrointestinal infections by EPEC and strict preventive measures should be adopted to decrease contamination of milk with EPEC and other bacteria originated from animals.

Isolation, characterization, and antimicrobial susceptibility pattern of Escherichia coli O157:H7 from foods of bovine origin in Mekelle, Tigray, Ethiopia

“…The present study also revealed an overall prevalence of 14.29% for Shiga toxin-producing E. coli O157:H7 (EHEC). This nding was almost in agreement with reports [73] (10.2%), [61] (10.4%), [74] (10.4%), [75] (10.9%), [76] (11.43%), [77] (17.47%), and [71] (18%). But, it was higher than the ndings of [78] (0.54%), [64] (1.92%), [79] (2.1%), [65] (2.4%), [80] (2.4%), [68] (2.9%), [81] (3.38%), [57] (3.5%), [82] (4.2%), [67] (5.2%), [58] (5.4%), [83] (6.3%), [84] (8.8%), [85] (9%), [60] (9.1%), and [86] (9.3%).…”

“…However, the highest level of resistance was observed against Amoxicillin (95.8%) which was in line with the reports of [60] (100%), and [78] (100%). In addition, 41.7% of the isolates were resistant to Tetracycline which was in agreement with the reports of [59] (47.6%) but was higher than the ndings of [92] (4%), [73] (5.1%), [77] (23.08%), [60] (27.7%), and [93] (32%). However, it was lower than the ndings of [81] (80.6%), [68] (81.8%), [67] (63.63%), and [91] (60.61%).…”

Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of Escherichia coli Isolated from Milk and Dairy Products in Isfahan, Iran

“…Based on the results, 10% of all milk and dairy product samples showed EPEC contamination. In another study in Iran, 17 out of 206 (8.25%) raw milk samples were positive for EPEC [ 42 ]. Moreover, in a study conducted in Brazil, a prevalence of 22.1% was reported for EPEC in pasteurized milk isolates [ 43 ].…”

First report of enteropathogenic and enteroinvasive <i>Escherichia coli</i> with multiple antibiotic resistance indices from African catfish (<i>Clarias glariepinus</i>) in Nigeria

“…It is important to state that the E. coli serogroups found in this study have been identified in humans to cause severe infections including fatal cases of infantile gastroenteritis (24). They have also been isolated from animals (dogs, rabbits, monkeys, sheep, birds) and food items such as vegetables and other food products from animal source such as raw milk or cheese (24,25). Barbosa and others recorded an overall prevalence of 43% EPEC serogroup from water and fresh fish in Brazil (9).…”