Isolation of an Adult Mouse Lung Mesenchymal Progenitor Cell Population

Abstract

Contained within the adult lung are differentiated mesenchymal cell types (cartilage, smooth muscle, and myofibrobasts) that provide structural support for airways and vessels. Alterations in the number and phenotype of these cells figure prominently in the pathogenesis of a variety of lung diseases. While these cells are thought to arise locally, progenitors have yet to be purified. In previous work, we developed a method for isolating progenitors from lung tissue: this technique takes advantage of the unique ability of cell populations enriched for somatic stem and progenitor activity to efflux the vital dye Hoechst 33342, a feature that permits isolation by flow cytometry-based procedures. Using this method, we determined that a rare population of mesenchymal progenitors resides within the CD45- CD31- Hoechst low fraction of the adult murine lung. Similar to other mesenchymal progenitors, these cells express Sca-1, CD106, and CD44; can be serially passaged; and can differentiate to smooth muscle, cartilage, bone, and fat. Overall, these findings demonstrate that a phenotypically distinct mesenchymal progenitor resides within the adult murine lung, and provide a scheme for their isolation and study.

Endothelial Side Population Cells Contribute to Tumor Angiogenesis and Antiangiogenic Drug Resistance

“…2F). Previous work by other investigators has demonstrated that lung SP cells from adult mice are composed of CD45 þ and CD45 À SP cells (26).…”

Autologous Lung-Derived Mesenchymal Stem Cell Transplantation in Experimental Emphysema

“…In a large-animal model of experimental emphysema, the lung-derived mesenchymal cell recently described by Karoubi et al and others (2,16,27,36). These cells evidence of tissue regeneration with increased blood perfusion and extracellular matrix (ECM) content was display regenerative capacity through expression of basement membrane proteins and growth factors.…”

“…Even with priming, only low-level BM-MSC retention has been observed in vivo (18,20). Furtherhave been isolated from various adult tissues including adipose, liver, lung, and heart, most studies evaluating more, therapeutic responses to BM-MSC transplantation have been limited to animal models of inflammatory their regenerative capacity have utilized bone marrowderived cells (17,25,29,33,36,40). Several lines of evilung diseases, such as asthma and acute lung injury (21,30,42).…”

Isolation & Characterization of Hoechst^low CD45^negative Mouse Lung Mesenchymal Stem Cells

“…The ability of lung MSC to differentiate into traditional mesenchymal lineages of osteoblast, adipocyte and chondrocyte and their cell surface expression of accepted mesenchymal markers is evaluated for each cell preparation. Cytogenetic banding analysis is also performed to confirm the absence of gross chromosomal abnormalities 2,3,[8][9][10][11] . …”