Isolation of actinomycetes from mangrove and estuarine sediments of Cochin and screening for antimicrobial activity

Rosmine, Emilda; Varghese, Saramma Aikkarakunnath

doi:10.12980/jclm.4.2016j5-148

Cited by 14 publications

(9 citation statements)

References 7 publications

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“…All the isolates were purified by continuous subculture on ISP2 medium and stored in 20% v/v glycerols. Isolation of mangrove sediment-associated actinobacteria was well documented in numerous reports after the completion of International Streptomyces Project 20, 21,9 . The potential actinobacterial strain producing antifungals (TSII) was identified by 16S rRNA gene sequence homology using bioinformatics tool (NBLAST) as Kutzneria sp.…”

Section: Resultsmentioning

confidence: 94%

Computational Biology Approaches Revealing Novel Target in Vascular Wilt Pathogen Fusarium oxysporum f. sp. lycopersici for the Ligands of Marine Actinobacterial Origin

Karuppiah¹,

Sree²,

Devi³

et al. 2020

J. Pure Appl. Microbiol.

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In an in vitro study of antagonistic mangrove actinobacteria for its antifungal compounds to eliminate Fusarium oxysporum f. sp. lycopersici, the extracellular metabolites from the actinobacterium Kutzneria sp. strain TSII was documented. A total of 24 useful ligands were identified when profiled with Gas Chromatography-Mass Spectrometry after purification through silica gel column chromatography. Molecular modeling of Short-chain Dehydrogenases / Reductases (SDRs) was performed in the prime module of Schrodinger 2018-4 software using SDR of Bacillus anthracis as a template (PDB: 3I3OA). In silico studies utilizing the molecular docking of the 24 ligands with SDR responsible for pathogenicity, distributed on the membrane of the Fusarium oxysporum were carried out in Schrodinger 2018-4. Results were indicated as 1,4-Benzenedicarboxylic acid, Bis(2-ethylhexyl) ester, Spiro[Cyclopentane-1,2'(1'H)-quinoxaline], Decanedioic acid, Didecyl ester with docking scores (Kcal/mol) of-8.151,-7.231,-6.031 and low binding free energy (ΔG b) values (Kcal/mol) of-68.11,-42.23,-79.41 respectively, relating to their potential use as antifungal agents in Fusarium wilt infections. As SDRs are involved in melanin biosynthesis, binding of these ligands may interfere with melanin biosynthetic pathways conferring pathogenicity to Fusarium oxysporum f. sp. lycopersici. Hence, the present research study uncovers in vitro antagonistic potential of marine actinobacterial metabolites towards the most devastating vascular wilt pathogen Fusarium oxysporum in tomatoes and identifies the probable involvement of SDR as the potential target in an in silico molecular docking approach.

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Section: Resultsmentioning

confidence: 94%

Computational Biology Approaches Revealing Novel Target in Vascular Wilt Pathogen Fusarium oxysporum f. sp. lycopersici for the Ligands of Marine Actinobacterial Origin

Karuppiah¹,

Sree²,

Devi³

et al. 2020

J. Pure Appl. Microbiol.

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“…Actinobacteria are widespread in all mangrove environments 1,36,37 . Several previous studies reports the occurrences of actinomycetes in different mangrove habitats from various geographical locations around the world 38 . Previous study showed 85 actinobacterial isolates from 60 sediment samples collected from mangrove rhizosphere on the Egyptian Red Sea coast 39 .…”

Section: Results and Discussion Distribution Of Marine Actinomycetes mentioning

confidence: 99%

“…Mangrove microorganisms, in particular actinobacteria, were reported to be capable of producing structurally unique bioactive natural products 1,36 . Previous study reported the presence of bioactive compounds (particularly antibiotics) in actinomycetes inhabiting the mangrove environment 38,48 . For examples, crude extracts of actinomycetes isolates (A5, A71 and A70) exhibited strong antimicrobial activity 39 Streptomyces sp.…”

Section: Antimicrobial Activitiesmentioning

confidence: 99%

Distribution and Characterization of Actinomycetes in Mangrove Habitats (Red Sea, Egypt) with Special Emphasis on Streptomyces mutabilis M3MT483919

Hamed¹,

Abdrabo²,

Fahmy³

et al. 2021

J Pure Appl Microbiol

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Ten sediment samples were gathered from several geographical locations around mangrove habitat, Red Sea coast, Egypt, during summer 2019. Actinobacteria are widespread in most mangrove soil samples. The average actinomycetes counts in sediment samples were ranged from 4 to 15 CFUg-1, also physico-chemical characters for soil samples were determined. Statistical analysis was applied to assess if the geographical location and physico-chemical characters influenced the communities of actinomycetes. A total of 10 actinomycetes were isolated and characterized physiologically and biochemically. The antimicrobial activities of different actinomycetes isolates were assessed. Isolate M3 was chosen as the most promising isolate with broad antagonistic activity against Bacillus subtilis ATCC 6633, Escherichia coli ATCC 19404, Staphylococcus aureus ATCC6538, Pseudomonas aeruginosa ATCC 9027, and Candida albicans ATCC 10231 with inhibition zones ranged from 12.0 ± 0.9 to 20.0 ± 1.9 mm. Genotypic characterization of isolate M3 was made using 16S rDNA sequence analysis and identified as Streptomyces mutabilis M3 with accession number MT483919. This strain exhibited anticancer activity against breast cancer cell line (Mcf7), liver cancer cell line (HepG2) and colon cancer cell line (HCT116) and the IC50 values were 324.77, 333.71 and 354.46, respectively. Streptomyces mutabilis M3 MT483919 had high bio-flocculating activity for seawater treatment, and the recovery of the samples ranged between 71.97 and 76.05%. The crude extract of Streptomyces mutabilis MT483919 M3 was analyzed by Fourier transform infrared spectrum (FT-IR) and Gas chromatography-mass spectrometry (GC-MS).

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“…They are affected by both marine in luences, such as waves, tides, a mixture of saline water, and sediments. As a result, they may contain many biological niches within a small area, and so are linked with large biological diversity (Parthasarathi et al, 2012;Rosmine and Varghese, 2016). Estuaries provide some of the most productive habitats on earth because of the accumulation and availability of nutrients along with adequate light conditions that enhance the production of phytoplankton in the water (Cloern et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Enzymatic potential of Streptomyces sp. KAS-1 isolated from the microbiologically unexplored estuary of Kali river ecosystem

Nayaka¹,

Nagaraja²,

Chakraborty³

et al. 2020

ijrps

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In the present study, a Streptomyces sp. KAS-1 was isolated from sediment soil samples of the Kali river estuary, Karwar, Karnataka, India. The isolated Actinomycetes strains were screened against pathogenic microorganisms and for the production of hydrolytic enzymes. Later, the strongest strain Streptomyces sp. KAS-1 was taken for further analysis based on primary characterizations. The morphological, physiological and biochemical characterizations of Streptomyces sp. KAS-1 were carried out, followed by molecular characterization through 16S rRNA gene sequencing. The 16S ribosomal RNA gene sequencing and analysis of the phylogenetic tree showed a 98.97% sequence similarity with Streptomyces thermocarboxydus strain SDT64 (KJ018992). Finally, the intracellular methanol extract was characterized through UV-Visible and FTIR spectrophotometer. The Streptomyces sp. is a gram-positive, aerobic, non-motile, spore-forming bacterium and it was designated as Streptomyces sp. KAS-1 and was found to possess a strong antimicrobial activity against pathogenic microorganisms. The characterizations revealed the Streptomyces sp. KAS-1 was moderately thermophilic, neutrophile organism having the capacity to degrade a broad range of carbon sources. The UV-Visible spectrum of methanol extract of Streptomyces sp. KAS-1 revealed the presence of Muconomycin-B and the presence of different functional groups such as aldehydes, amines, and alkyl halides were indicated by the FTIR spectrum.

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Isolation of actinomycetes from mangrove and estuarine sediments of Cochin and screening for antimicrobial activity

Cited by 14 publications

References 7 publications

Computational Biology Approaches Revealing Novel Target in Vascular Wilt Pathogen Fusarium oxysporum f. sp. lycopersici for the Ligands of Marine Actinobacterial Origin

Computational Biology Approaches Revealing Novel Target in Vascular Wilt Pathogen Fusarium oxysporum f. sp. lycopersici for the Ligands of Marine Actinobacterial Origin

Distribution and Characterization of Actinomycetes in Mangrove Habitats (Red Sea, Egypt) with Special Emphasis on Streptomyces mutabilis M3MT483919

Antimicrobial and Enzymatic potential of Streptomyces sp. KAS-1 isolated from the microbiologically unexplored estuary of Kali river ecosystem

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