Isolation of a Novel Ubiquitin-like Protein from Pleurotus ostreatus Mushroom with Anti-Human Immunodeficiency Virus, Translation-Inhibitory, and Ribonuclease Activities

Wang, H.X.; Ng, T.B.

doi:10.1006/bbrc.2000.3540

Cited by 88 publications

(36 citation statements)

References 40 publications

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“…Clone MV094 coded for a conserved ubiquitin core fused to 58 aminoacids homologous to the S27 ribosomal protein. This protein diVers from that described by Wang and Ng in P. ostreatus (Wang and Ng, 2000), but its structure has been also found in other fungi such as Ustilago maydis and N. crassa, animals and plants.…”

Section: Protein Metabolismcontrasting

confidence: 53%

Genetic linkage map and expression analysis of genes expressed in the lamellae of the edible basidiomycete Pleurotus ostreatus

Peñas¹,

Ramı́rez²,

Pisabarro³

2006

Fungal Genetics and Biology

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Section: Protein Metabolismcontrasting

confidence: 53%

Genetic linkage map and expression analysis of genes expressed in the lamellae of the edible basidiomycete Pleurotus ostreatus

Peñas¹,

Ramı́rez²,

Pisabarro³

2006

Fungal Genetics and Biology

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“…Ubiquitin-like peptides from the fruiting bodies of edible mushrooms, including Pleurotus sajor-caju, Pleurotus ostreatus, and Cantharellus cibarius, have been characterized so far (29,47,48). These peptides show an N-terminal sequence identical to that of ubiquitin, and all have RNaseand translation-inhibiting activities.…”

Section: Discussionmentioning

confidence: 99%

Pichia anomalaDBVPG 3003 Secretes a Ubiquitin-Like Protein That Has Antimicrobial Activity

Ingeniis

Raffaelli

Ciani

et al. 2009

Appl Environ Microbiol

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The yeast strain Pichia anomala DBVPG 3003 secretes a killer toxin (Pikt) that has antifungal activity against Brettanomyces/Dekkera sp. yeasts. Pikt interacts with ␤-1,6-glucan, consistent with binding to the cell wall of sensitive targets. In contrast to that of toxin K1, secreted by Saccharomyces cerevisiae, Pikt killer activity is not mediated by an increase in membrane permeability. Purification of the toxin yielded a homogeneous protein of about 8 kDa, which showed a marked similarity to ubiquitin in terms of molecular mass and N-terminal sequences. Pikt is also specifically recognized by anti-bovine ubiquitin antibodies and, similar to ubiquitin-like peptides, is not absorbed by DEAE-cellulose. However, Pikt differs from ubiquitin in its sensitivity to proteolytic enzymes. Therefore, Pikt appears to be a novel ubiquitin-like peptide that has killer activity.After its initial discovery in Saccharomyces cerevisiae (2), the killer phenotype has been found in yeasts belonging to the genera Candida, Cryptococcus, Debaryomyces, Hanseniaspora, Hansenula, Kloeckera, Kluyveromyces, Metschnikowia, Pichia, Rhodotorula, Schwanniomyces, Torulopsis, Trichosporon, Williopsis, and Zygosaccharomyces (9,16,19,25,28,53,56). The distribution of this character among natural yeast isolates and in laboratory strains is accompanied by killer toxins with large biodiversity, in terms of their biochemical characteristics, genetic determinants, and spectra of action (25). In spite of this diversity, all known killer toxins are proteins (1, 31) or glycoproteins (5,11,28,43,54) that kill sensitive cells via a two-step mode of action. During the first step, the killer toxin binds a receptor site on the cell wall of its sensitive target. In the second step, which has been far less characterized, the killer toxin is assumed to interact with receptors on the cell membrane and to kill the sensitive cells via different mechanisms. These include cell membrane permeabilization, cell cycle perturbation, inhibition of DNA synthesis, and inhibition of ␤-1,3-glucan synthetase activity (7,14,15,41,49,53).As the spectrum of action of some toxins has extended to microbial pathogens of clinical interest, killer toxins and/or killer toxin-like antibodies and mimotopes are of great relevance to medicine (8,25). Other toxins that exert a killing action on spoilage yeasts have interesting applications in the fermentative (46) and food and feed industries (10,11,12,23,32,44), where they can be used as "natural" food antimicrobials.In a previous study, we showed that Pichia anomala DBVPG 3003 produces a killer toxin (known as Pikt) that is active against Dekkera/Brettanomyces sp. yeasts (12). These yeasts can develop in white and red wines, resulting in unpleasant odors and tastes (18). Thus, they represent a major problem in the wine industry. Pikt is stable at acidic pH and in a range of temperatures between 20°C and 40°C (12), as are other toxins produced by different strains of P. anomala (25,26,37). Moreover, Pikt maintains its killing activity for ...

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“…wt. 14.5 kDa) were isolated and characterized from sclerotia of P. tuber-regium, exhibited very stable nuclease activity at 100°C for 30 minutes with a higher ribonucleolytic activity toward poly-G [39,40,41]. Another ribonuclease, pleuturegin, was also isolated from both fresh and dried sclerotia of P. tuber-regium [17].…”

Section: Antihuman Immunodeficiency Virus (Hiv) Activitymentioning

confidence: 99%

Oyster Mushroom : Answer to Human Ailments:a Review

Jandaik¹,

Sharma²

2017

Asian J Pharm Clin Res

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Oyster mushroom is consumed all over the world due to its taste, flavor, high nutritional value, and some medicinal properties. Many species of this genus are rich in proteins with essential amino acids, polysaccharides, essential amino acids, dietary fibers, important minerals, and some vitamins. Because of this nutritional composition and presence of bioactive molecules, oyster mushroom has been reported to have anticancer, antihypertensive, antihypercholestromic, antidiabetic, antiobesity, antiaging, antimicrobial, and antioxidant activities. The high nutritional value and potent medicinal uses suggest that Pleurotus mushrooms are important functional foods or nutraceuticals.

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Isolation of a Novel Ubiquitin-like Protein from Pleurotus ostreatus Mushroom with Anti-Human Immunodeficiency Virus, Translation-Inhibitory, and Ribonuclease Activities

Cited by 88 publications

References 40 publications

Genetic linkage map and expression analysis of genes expressed in the lamellae of the edible basidiomycete Pleurotus ostreatus

Genetic linkage map and expression analysis of genes expressed in the lamellae of the edible basidiomycete Pleurotus ostreatus

Pichia anomalaDBVPG 3003 Secretes a Ubiquitin-Like Protein That Has Antimicrobial Activity

Oyster Mushroom : Answer to Human Ailments:a Review

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