Isolation of a full-length CC–NBS–LRR resistance gene analog candidate from sugar pine showing low nucleotide diversity

Jermstad, K. D.; Sheppard, Lorraine; Kinloch, Bohun B.; Delfino-Mix, Annette; Ersoz, Elhan S.; Krutovsky, Konstantin V.; Neale, David B.

doi:10.1007/s11295-005-0029-6

Cited by 23 publications

(10 citation statements)

References 72 publications

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“…A previously cloned Pinus lambertiana NBS-LRR gene is highly conserved, even between sugar pine and WWP (Jermstad et al 2006). Although an AFLP-based method was useful in discovering RGA-related DNA markers specific to disease resistant trees Ekramoddoullah 2007, 2008), it is still a challenge to identify full-length RGAs and to elucidate their genetic variation and molecular role in disease resistance in a conifer pathosystem.…”

Section: Discussionmentioning

confidence: 99%

Genomic organization, induced expression and promoter activity of a resistance gene analog (PmTNL1) in western white pine (Pinus monticola)

Liu

Ekramoddoullah

2011

Planta

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Cronartium ribicola causes white pine blister rust (WPBR) in subgenus Strobus. Various genetic and molecular approaches were used to detect white pine genes contributing to host resistance. The molecular role of the NBS-LRR family is highly related to plant immuno-activity against various pathogens and pests. In the present study, genomic organization of a resistance gene analog (RGA), designated as PmTNL1, and its allelic variants were characterized in Pinus monticola. PmTNL1 showed high identity with TIR-NBS-LRR proteins from other plants. qRT-PCR revealed that the PmTNL1 transcript was expressed at low basal levels in different tissues and exhibited similar patterns during compatible and incompatible interactions of P. monticola with C. ribicola at early stages post inoculation. In comparison, PmTNL1 was up-regulated significantly in diseased P. monticola tissues with WPBR symptoms. Expression of the PmTNL1 promoter::GUS fusion gene in transgenic Arabidopsis demonstrated that GUS signal appeared only inside phloem tissues of young seedlings and at hydathodes and branching and organ-connecting points in mature Arabidopsis plants. Similar to the endogenous expression pattern for this gene in pine, GUS activity was up-regulated significantly around vascular tissues locally at pathogen infection sites, but little or no induction was observed in response to abiotic stresses. A DNA marker was developed based on variation of the LRR-coding region, and PmTNL1 was mapped to one genetic linkage group using a pedigree with major dominant gene (Cr2) conferring HR resistance to C. ribicola. These results suggest that PmTNL1 may play an important role in white pine partial resistance against C. ribicola.

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Section: Discussionmentioning

confidence: 99%

Genomic organization, induced expression and promoter activity of a resistance gene analog (PmTNL1) in western white pine (Pinus monticola)

Liu

Ekramoddoullah

2011

Planta

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“…In sugar pine, the R gene associated with a hypersensitive reaction (HR) response has been mapped by proximity to six markers (D evey et al. 1995; also see J ermstad et al. 2006), thereby providing a beginning point for gene cloning.…”

Section: Pathology and Ecologymentioning

confidence: 99%

White pines, Ribes, and blister rust: a review and synthesis

2010

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For over a century, white pine blister rust (Cronartium ribicola) has linked white pines (Strobus) with currants and gooseberries (Ribes) in a complex and serious disease epidemic in Asia, Europe, and North America. Because of ongoing changes in climate, societal demands for forests and their amenities, and scientific advances in genetics and proteomics, our current understanding and management of the white pine blister rust pathosystem has become outdated. Here, we present a review and synthesis of international scope on the biology and management of blister rust, white pines, Ribes, and other hosts. In this article, we provide a geographical and historical background, describe the taxonomy and life cycle of the rust, discuss pathology and ecology, and introduce a series of invited papers. These review articles summarize the literature on white pines, Ribes, and blister rust with respect to their status, threats, and management through genetics and silviculture. Although the principal focus is on North America, the different epidemics in Europe and Asia are also described. In the final article, we discuss several of the key observations and conclusions from the preceding review articles and identify prudent actions for research and management of white pine blister rust.

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“…The reports also suggested that the R genes from conifers and angiosperms share common ancestry and were under diversifying selection and/or neutral selection (Liu and Ekramoddoullah 2003). Subsequently, a 2.9 kb CC-NBS-LRR subclass of RGA with low nucleotide polymorphism in the NBS-LRR domain was reported by Jermstad et al (2006)The gene was 58 times less variable than other R genes from conifers. This suggested that the gene acted as 'guardee' protein which generally maintains low number of alleles in the populations (Maurico et al 2003;McDowell 2004).…”

Section: Hydrolysis Of Chitinmentioning

confidence: 99%

Pathogenesis-related genes and proteins in forest tree species

Veluthakkal

Dasgupta

2010

Trees

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Trees occupy more than 30% of the land biosphere. They are important from both ecological and environmental standpoints and provide some of the most valuable commodities in the world economy. The perennial nature and size of trees are the critical determinants of their survival in response to biotic and abiotic stresses. The identification of the defense pathways at biochemical and genetic levels in tree pathosystems are beginning to be addressed. The basic physiological and biochemical mechanisms in woody perennials in response to pathogen is homologous to the model annual crop like Arabidopsis, but their secondary metabolic processes and ecological survival strategies are likely to be divergent from their annual counterparts. The limited domestication in tree species makes its molecular mechanisms less comparable to the highly pedigreed crop species. Recent reports have highlighted that the possible difference in genetic programs responding to invasive pathogens between annuals and perennials could be the spatial and temporal pattern of gene regulation. Several reviews on pathogen defense with reference to crop species are available, while similar reports from the tree species are limited to few commercially important species like Populus, Pinus, Picea, Eucalyptus, Castanea, and Pseudotsuga. This paper reviews the present status of pathogenesis-related genes and proteins from tree species with emphasis on the resistant genes and the proteins induced during systemic acquired resistance and highlights the ecological and evolutionary significance of defense-related genes from tree species.

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Isolation of a full-length CC–NBS–LRR resistance gene analog candidate from sugar pine showing low nucleotide diversity

Cited by 23 publications

References 72 publications

Genomic organization, induced expression and promoter activity of a resistance gene analog (PmTNL1) in western white pine (Pinus monticola)

Genomic organization, induced expression and promoter activity of a resistance gene analog (PmTNL1) in western white pine (Pinus monticola)

White pines, Ribes, and blister rust: a review and synthesis

Pathogenesis-related genes and proteins in forest tree species

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