Isolation of a chromosomally engineered durum wheat line carrying theAegilops ventricosa Pch1gene for resistance to eyespot

Abstract

The chromosome 7Dv of Aegilops ventricosa (syn. Triticum ventricosum, 2n = 4x = 28, genome DvDvMvMv) carries the gene Pch1 for resistance to eyespot. This gene has previously been transferred to chromosome 7D of bread wheat, T. aestivum (2n = 6x = 42, genome AABBDD). To (1) enhance the level of resistance of bread wheat by increasing the copy number of Pch1, and (2) create eyespot-resistant triticales, meiotically stable Pch1-carrying durum lines were selected from the backcross progenies of a cross between Ae. ventricosa and T. durum cv. Creso ph1c (2n = 4x = 28, genome AABB). The Pch1 transfer, likely resulting from homoeologous recombination, was located at the distal position on the long arm of chromosome 7A. The 7A microsatellite marker Xgwm 698 was found closely linked in repulsion to the introgression in the resistant recombination lines, and the endopeptidase allele located on chromosome 7A of cv. Creso ph1c was lost.

The Lr34 adult plant rust resistance gene provides seedling resistance in durum wheat without senescence

“…Several rust resistance genes have been transferred from durum wheat into hexaploid wheat due to the relative simplicity of crossing these species and selecting fertile hexaploid lines or alternatively producing synthetic hexaploid wheat by crossing tetraploid wheat (AABB) with Aegilops tauschii (DD). Germplasm development in tetraploid wheat using interspecies crosses, while achievable (Huguet‐Robert et al ., ; Klindworth et al ., ; Morris et al ., ), is more difficult due to poor vigour and low fertility in tetraploid backgrounds (Ceoloni et al ., ; Klindworth et al ., ). Of particular difficulty is the introduction of D genome genes of hexaploid wheat or Ae.…”

Characterization of Aegilops kotschyi Boiss. x Triticum aestivum L. hybrid lines

“…In the analyses, fourteen ISSR selected primers were used. H u g u e t -R o b e r t et al [45] used microsatellite markers to locate the gene Ph 1 for resistance to eyespot in the resistant Ae. ventricosa Tausch.…”

Advances and Prospects in Wheat Eyespot Research: Contributions from Genetics and Molecular Tools

“…This is perhaps due to the fact that the Ae. ventricosa accession used to develop these lines was different from the accession used to obtain VPM, and the associated allele at the linked isozyme locus is a null allele in these sources (Huguet‐Robert et al. 2001; J. Jahier, personal communication).…”