Isolation, identification and characterization of apigenin from Justicia gendarussa and its anti-inflammatory activity

Kumar, Krishna B.; Sabu, Vidya; Sindhu, Ganapathy; Rauf, Arun A.; Helen, A.

doi:10.1016/j.intimp.2018.04.004

Cited by 52 publications

(17 citation statements)

References 40 publications

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“…In other Justicia species, it is possible to identify immunomodulatory characteristics, as described by Kumar et al [47] when reporting the inhibition of paw edema after treatment with fractions from ethyl-acetate fraction of Justicia gendarussa . In another study using the carrageenan and formalin-induced paw edema models, it was found a dose-dependent response after treatment with methanolic extract of Justicia secunda leaf at carrageenan paw edema models, indicating the significant inhibition after 2 h, 3 h, and 24 h treatment at 0.1, 0.2, and 0.4 g/kg [48].…”

Section: Resultsmentioning

confidence: 99%

Organic Extract of Justicia pectoralis Jacq. Leaf Inhibits Interferon‐γ Secretion and Has Bacteriostatic Activity against Acinetobacter baumannii and Klebsiella pneumoniae

Nunes

Cordeiro

Beserra

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Justicia pectoralis Jacq. (Acanthaceae) leaves currently found in the Brazilian north-east are widely used to treat diabetes, menstrual pains, asthma, and other disorders. This work aimed to identify the phytochemical characterization and biological activities of J. pectoralis leaf extracts. The plant material was ground and the crude extracts were obtained with water or acetone: water (7:3 v/v), yielding aqueous (JPA), and organic (JPO) extracts. Phytochemical characterization was performed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). Cytotoxicity was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay and trypan blue (TB) exclusion assay in peripheral blood mononuclear cells (PBMCs), BALB/c splenocytes, and neoplastic cells (TOLEDO, K562, DU-145, and PANC-1) at 1, 10, and 100 μg/mL. Antibacterial activity was evaluated using the microdilution test to obtain the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Cytokines, IFN-γ, and IL-17A from culture supernatants of BALB/c mice splenocytes were measured by sandwich ELISA. In the TLC analysis, both JPA and JPO extracts presented coumarin and flavonoids. In addition, HPLC was able to identify coumarin, apigenin, and ellagic acid in both extracts. JPO IC50 was 57.59 ± 1.03 μg/mL (MTT) and 69.44 ± 8.08 μg/mL (TB) in TOLEDO. MIC value of JPO against Acinetobacter baumannii and Klebsiella pneumoniae was 500 μg/mL. JPO (100 μg/mL) significantly inhibited IFN-γ levels (p=0.03). J. pectoralis is a potential candidate to be further investigated as an IFN-γ inhibitory agent and against Acinetobacter baumannii and Klebsiella pneumoniae.

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Section: Resultsmentioning

confidence: 99%

Organic Extract of Justicia pectoralis Jacq. Leaf Inhibits Interferon‐γ Secretion and Has Bacteriostatic Activity against Acinetobacter baumannii and Klebsiella pneumoniae

Nunes

Cordeiro

Beserra

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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“…fractionated then isolated to obtain apigen compound. This study stated 89% inhibition of edema at dose of 10 mg/kg in carrageenan induced rat [17].…”

Section: Anti Inflamationmentioning

confidence: 68%

Overview of Phytochemical and Pharmacological of Gandarussa Extract (Justicia Gendarussa Burm)

Putri¹,

Zulharmita²,

Asra³

et al. 2020

EASJPP

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“…The epigenin extract from the root of the plant Justicia gendarussa by using the methanol showed the anti-in lammatory action on the Human Peripheral Blood Mononuclear Cells (hPBMCs) induced with ox-LDL method. Epigenin has been shown the anti-in lammatory activity by the inhibition of NF-kB pathway (Kumar et al, 2018). Ethyl acetate fraction which is isolated from the plant Justicia gendarussa roots extracted by methanol showed the signi icant anti-in lammatory activity on rats by carrageenan induced rat paw edema at the dose of 50mg/kg.…”

Section: Anti-in Lammatory Rolementioning

confidence: 99%

Pharmacognostical and pharmacological activity of Justicia gendarussa burm.f.: a comprehensive review

Ashish¹,

Anjali²,

K³

et al. 2020

ijrps

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Justicia gendarussa Burm .f. (family Acanthaceae) which is also known as willow-leaves and commonly known as Nili-Nirgundi, it is very commonly found nearby to China and its availability is very common in larger parts of India and Andaman islands. Traditionally it is used to treat various sorts of disorders such as wound healing, anti-inflammatory, anti-oxidant, antiproliferative, anti-arthritic etc. Justicia gendarussa is one of the crucial herbs which has been used in the Ayurveda. Majorly leaves parts of the plant shows the pharmacological activity but the root of the plant Justicia gendarussa is also have the important medicinal values. A large variety of pharmacologically active constituents i.e., alkaloids, flavonoids, saponin, carbohydrates, steroids, triterpenoids, carotenoids, aminoacids, tannins, phenolics, coumarines and anthaquinones are also present in this plant and they makes the plant pharmacologically important. The activity of the plant is also dependent on the solvent which is used for the extraction the various vital chemical constituents. The different- different parts of the plants having the different medicinal values also differ in the chemical values. This review is not only focused on the essential phytochemical constituents which is available in the plant but it also explains their necessary medicinal value to shows the essential biological action and phytopharmacological actions of various parts of the plant.

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Isolation, identification and characterization of apigenin from Justicia gendarussa and its anti-inflammatory activity

Cited by 52 publications

References 40 publications

Organic Extract of Justicia pectoralis Jacq. Leaf Inhibits Interferon‐γ Secretion and Has Bacteriostatic Activity against Acinetobacter baumannii and Klebsiella pneumoniae

Organic Extract of Justicia pectoralis Jacq. Leaf Inhibits Interferon‐γ Secretion and Has Bacteriostatic Activity against Acinetobacter baumannii and Klebsiella pneumoniae

Overview of Phytochemical and Pharmacological of Gandarussa Extract (Justicia Gendarussa Burm)

Pharmacognostical and pharmacological activity of Justicia gendarussa burm.f.: a comprehensive review

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